Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-18T20:18:37.935Z Has data issue: false hasContentIssue false
  • English
  • Français

Synthesis of extrachromosomal DNA and telomere-related sequences in germinating wheat embryos

Published online by Cambridge University Press:  19 September 2008

M. Bucholc  and
J. Buchowicz
M. Bucholc
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 36 Rakowiecka, 02-532 Warsaw, Poland
J. Buchowicz*
Affiliation:
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 36 Rakowiecka, 02-532 Warsaw, Poland
*
* Correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

Nuclear DNA of wheat embryos was probed with a synthetic oligonucleotide (C3TA3C3TA3) to assess changes in the length of telomeres during storage and germination. Chromosomal DNA of dry embryos, especially those derived from long-term-stored caryopses, was considerably depleted of telomeric repeats, but, shortly after the start of imbibition, the chromosomal DNA became rich in sequences hybridizing to the telomeric probe. Simultaneously, newly synthesized telomere-related sequences appeared in an extrachromosomal form that could be separated from bulk nuclear DNA by velocity and isopycnic centrifugations. It is concluded that, in addition to typical repair events, rapid synthesis of telomeric repeats occurs in wheat embryos at the onset of germination.

Keywords

extrachromosomal DNAgerminationsenescencetelomereTriticum aestivum L.
Type
Research Papers
Information
Seed Science Research , Volume 2 , Issue 3 , September 1992 , pp. 141 - 146
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ausubel, F.M., Brent, R., Kingston, R.E., More, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1987) Current protocols in molecular biology. New York, Greene Publishing Associates and John Wiley and Sons.Google Scholar
Bewley, J.D. and Black, M. (1985) Seeds. Physiology of development and germination. New York, Plenum Press.CrossRefGoogle Scholar
Blackburn, E.H. (1990) Telomeres: structure and synthesis. Journal of Biological Chemistry 265, 5919–5921.CrossRefGoogle Scholar
Blackburn, E.H. (1991) Structure and function of telomeres. Nature 350, 569–573.CrossRefGoogle ScholarPubMed
Boeke, J.D. (1990) Reverse transcriptase, the and of chromosome, and the end of life. Cell 61, 193–195.CrossRefGoogle ScholarPubMed
Bucholc, M. and Buchowicz, J. (1986) Extensive DNA repair in early whneat embryos. pp. 204–208 in Bakardjieva, N. (Ed.) Self-Regulation of plant metabolism, Vol. 2. Sofia, Bulgarian Academy of Sciences.Google Scholar
Bucholc, M. and Buchowicz, J. (1989) Replicative, repair, and third type of nuclear DNA synthesis in germinating wheat embryos. Acta Physiologiae Plantarum 11, 13–18.Google Scholar
Elder, R.H., Dell'Aquila, A., Mezzina, M., Sarasin, A. and Osborne, D.J. (1987) DNA ligase in repair and replication in the embryos of rye, Secale cereale. Mutation Research 181, 61–71.CrossRefGoogle Scholar
Hattori, J., Gottlob-McHugh, S.G. and Johnson, D.A. (1987) The isolation of high-molecular-weight DNA from plants. Analytical Biochemistry 165, 70–74.CrossRefGoogle ScholarPubMed
Marciniak, B., Bucholc, M. and Buchowicz, J. (1987) Early DNA synthesis during the germination of wheat embryos. Phytochemistry 26, 331–334.CrossRefGoogle Scholar
Osborne, D.J. (1982) DNA integrity and repair in seed germination: the importance in viability and survival. pp. 435–463 in Khan, A.A. (Ed.) The physiology and biochemistry of seed development, dormancy and germination. Amsterdam, Elsvier Biomedical Press.Google Scholar
Richards, E.J. and Ausubel, F.M. (1988) Isolation of a higher eukaryotic telomere from Arabidopsis thaliana. Cell 53, 127–136.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: a laboratory manual. pp. 1.36–1.37. Cold Spring Harbor, CSH Laboratory Press.Google Scholar
Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98, 503–517.CrossRefGoogle ScholarPubMed
Tomaszewski, M., Dobrzanska, M., Gozdzicka-Jozefiak, A., Materny, M. and Buchowicz, J. (1991) Detection of a DNA segment that occurs in wheat embryo but not wheat endosperm cell nuclei. Plant Science 73, 175–179.CrossRefGoogle Scholar
Vazquez-Ramos, J.M. and Osborne, D.J. (1986) Analysis of the DNA synthesized during early germination of rye embryos using BND-cellulose chromatography. Mutation Research 166, 39–47.CrossRefGoogle Scholar
Wang, S.-S. and Zakian, V.A. (1990) Telomere-telomere recombination provides an express pathway for telomere acquisition. Nature 345, 456–458.CrossRefGoogle ScholarPubMed
Zakian, V.A. (1989) Structure and function of telomeres. Annual Review of Genetics 23, 579–604.CrossRefGoogle ScholarPubMed
Zarain, M., Bernal-Lugo, I. and Vazquez-Ramos, J.M. (1987) Effect of benzyladenine on the DNA synthesis during early germination of maize embryo axes. Mutation Research 181, 103–110.Google Scholar
Zlatanova, J.S., Ivanov, P.V., Stoilov, L.M., Chimshirova, K.V. and Stanchev, B.S. (1987) DNA repair precedes replicative synthesis during early germination in maize. Plant Molecular Biology 10, 139–144.CrossRefGoogle ScholarPubMed