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The relationship between the mitotic activity and moisture content of recalcitrant seeds of Acer saccharinum (L.) during maturation, post-maturation drying and germination

Published online by Cambridge University Press:  22 February 2007

L.E. Kozeko  and
V.M. Troyan
L.E. Kozeko*
Affiliation:
N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereshchenkivska 2, 252601 Kiev, Ukraine
V.M. Troyan
Affiliation:
N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereshchenkivska 2, 252601 Kiev, Ukraine
*
*Correspondence Email: [email protected]
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Abstract

The decline of embryo moisture content from approx. 82 to 53% in 1997 and 56% in 1998 in recalcitrant seeds of Acer saccharinum during maturation was accompanied by decreased mitotic activity in the meristems and an increase in the percentage of cells in the G1 phase of the cell cycle. DNA synthesis and mitosis in the root apex ceased at approx. 53% embryo moisture content, and 67% of the cells were arrested in the G1 phase. During post-maturation drying, cell division in the shoot apex and embryonic leaves continued as long as the embryo moisture content was higher than 50 and 45%, respectively. Mitotic activity in the drying embryo may be controlled by its moisture level. Increased proliferation of the root, shoot and leaf meristems of the mature seeds was already recorded at 24 h of germination, before the root protruded through the seed coat. However, the increase in the embryo moisture content was small – from 56 to 59%. In the 3 d seedlings (10–15 mm long) the mitotic index reached 8% in the root apex and 12% in the shoot apex with leaves. Placing mature seeds in moist conditions might be necessary for the switch from proliferation decline towards its activation. Thus, in contrast with orthodox seeds, the preservation of cell division capacity and increased mitotic activity may be essential for rapid germination immediately after shedding in mature Acer seeds.

Keywords

Acer saccharinumdormancyElymus elymoidesgerminationmaturationmitotic activitymoisture contentrecalcitrant seed
Type
Research Article
Information
Seed Science Research , Volume 10 , Issue 3 , September 2000 , pp. 225 - 232
Copyright
Copyright © Cambridge University Press 2000

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