Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-23T21:37:25.678Z Has data issue: false hasContentIssue false
  • English
  • Français

Recalcitrant and orthodox Spartina seeds or isolated embryos exhibit similar leachate patterns immediately following desiccation

Published online by Cambridge University Press:  25 March 2015

James H. Chappell Jr ,
Yi Wang  and
Marc Alan Cohn
James H. Chappell Jr
Affiliation:
Department of Plant Pathology and Crop Physiology, 302 Life Sciences Building, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
Yi Wang
Affiliation:
Department of Plant Pathology and Crop Physiology, 302 Life Sciences Building, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
Marc Alan Cohn*
Affiliation:
Department of Plant Pathology and Crop Physiology, 302 Life Sciences Building, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
*
*Correspondence E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Solute leakage is used as an indicator of membrane damage during desiccation of recalcitrant seeds. We re-examined this phenomenon and its utility by comparing recalcitrant Spartina alterniflora and orthodox Spartina pectinata seeds, as well as drying isolated embryos or embryos isolated after whole-seed drying. During drying, intact seed leakage (electrical conductivity and absorbance at 280 nm) was independent of seed moisture content, dormant/non-dormant state or desiccation tolerance. Embryos from both Spartina species, isolated after drying within the intact seed, exhibited increased leakage, especially below 40% (dry weight basis, DWB) seed moisture, the critical water content for viability loss in Spartina alterniflora. When isolated embryos were dried, the pattern and extent of increased solute leakage were similar in both Spartina species, even though S. pectinata embryos were >95% viable and S. alterniflora embryos were < 20% viable. We conclude that increased solute leakage is an artefact of embryo excision and not an accurate indicator of desiccation damage to recalcitrant Spartina alterniflora seeds.

Keywords

desiccation toleranceelectrical conductivitymembrane damagerecalcitrant seedssolute leakageSpartinaviability
Type
Research Papers
Information
Seed Science Research , Volume 25 , Issue 3 , September 2015 , pp. 247 - 254
Check for updates
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Becwar, M.R., Stanwood, P.C. and Roos, E.E. (1982) Dehydration effects on imbibitional leakage from desiccation-sensitive seeds. Plant Physiology 69, 1132–1135.CrossRefGoogle ScholarPubMed
Berjak, P. and Pammenter, N. (2000) What ultrastructure has told us about recalcitrant seeds. Revista Brasileira de Fisiologia Vegetal 12, 22–55.Google Scholar
Berjak, P., Farrant, J.M. and Pammenter, N.W. (1989) The basis of recalcitrant seed behavior. Cell biology of the homoiohydrous seed condition. pp. 89–108 in Taylorson, R.B. (Ed.) Recent advances in the development and germination of seeds. New York, Plenum Press.CrossRefGoogle Scholar
Berjak, P., Pammenter, N.W. and Vertucci, C. (1992) Homoiohydrous (recalcitrant) seeds: developmental status, desiccation sensitivity and the state of water in axes of Landolphia kirkii Dyer. Planta 186, 249–261.CrossRefGoogle ScholarPubMed
Bilia, D.A.C., Marcos, J. and Novembre, A.D.C.L. (1999) Desiccation tolerance and seed storability of Inga uruguensis (Hook. et Arn.). Seed Science and Technology 27, 77–89.Google Scholar
Bonner, F.T. (1996) Responses to drying of recalcitrant seeds of Quercus nigra L. Annals of Botany 78, 181–187.CrossRefGoogle Scholar
Chaitanya, K.S.K. and Naithani, S.C. (1994) Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robusta Gaertn. f. New Phytologist 126, 623–627.CrossRefGoogle Scholar
Chandel, K.P.S., Chaudhury, R., Radhamani, J. and Malik, S.K. (1995) Desiccation and freezing sensitivity in recalcitrant seeds of tea, cocoa and jackfruit. Annals of Botany 76, 443–450.CrossRefGoogle Scholar
Chappell, J.H. (2008) Is oxidative stress the cause of death when recalcitrant Spartina alterniflora seeds are dried? PhD dissertation, Louisiana State University, Baton Rouge.Google Scholar
Chappell, J.H. and Cohn, M.A. (2011) Corrections for interferences and extraction conditions make a difference: use of the TBARS assay for lipid peroxidation of orthodox Spartina pectinata and recalcitrant Spartina alterniflora seeds during desiccation. Seed Science Research 21, 153–158.CrossRefGoogle Scholar
Chaudhury, R. and Malik, S.K. (2004) Desiccation and freezing sensitivity during seed development in jackfruit. Seed Science and Technology 32, 785–795.CrossRefGoogle Scholar
Côme, D. and Corbineau, F. (1996) Metabolic damage related to desiccation sensitivity. pp. 83–97 in Ouédraogo, A.S.; Poulsen, K.; Stubsgaard, F. (Eds) Intermediate/recalcitrant tropical forest tree seeds. Rome, IPGRI, Available at http://www2.bioversityinternational.org/publications/Web_version/67/ (accessed accessed November 2010).Google Scholar
Finch-Savage, W.E., Blake, P.S. and Clay, H.A. (1996) Desiccation stress in recalcitrant Quercus robur L. seeds results in lipid peroxidation and increased synthesis of jasmonates and abscisic acid. Journal of Experimental Botany 47, 661–667.CrossRefGoogle Scholar
Fu, J.R., Zhang, B.Z., Wang, X.P., Qiao, Y.Z. and Huang, X.L. (1990) Physiological studies on desiccation, wet storage and cryopreservation of recalcitrant seeds of three fruit species and their excised embryonic axes. Seed Science and Technology 18, 743–754.Google Scholar
Hibbard, R.P. and Miller, E.V. (1928) Biochemical studies of seed viability. I. Measurements of conductance and reduction. Plant Physiology 3, 335–352.CrossRefGoogle ScholarPubMed
Hoekstra, F.A., Golovina, E.A., Van Aelst, A.C. and Hemminga, M.A. (1999) Imbibitional leakage from anhydrobiotes revisited. Plant, Cell and Environment 22, 1121–1131.CrossRefGoogle Scholar
Kundu, M. and Kachari, J. (2000) Desiccation sensitivity and recalcitrant behavior of seeds of Aquilaria agallocha Roxb. Seed Science and Technology 28, 755–760.Google Scholar
Lan, Q.Y., Jiang, X.C., Song, S.Q., Lei, Y.B. and Yin, S.H. (2007) Changes in germinability and desiccation-sensitivity of recalcitrant Hopea hainanensis Merr. et Chun seeds during development. Seed Science and Technology 35, 21–31.CrossRefGoogle Scholar
Leopold, A.C., Musgrave, M.E. and Williams, K.M. (1981) Solute leakage resulting from leaf desiccation. Plant Physiology 68, 1222–1225.CrossRefGoogle ScholarPubMed
Li, C.R. and Sun, W.Q. (1999) Desiccation sensitivity and activities of free radical-scavenging enzymes in recalcitrant Theobroma cacao seeds. Seed Science Research 9, 209–217.CrossRefGoogle Scholar
Nautiyal, A.R. and Purohit, A.N. (1985a) Seed viability in sal. III. Membrane disruption in aging seeds of Shorea robusta . Seed Science and Technology 13, 77–82.Google Scholar
Nautiyal, A.R. and Purohit, A.N. (1985b) Seed viability in sal. II. Physiological and biochemical aspects of aging in seeds of Shorea robusta . Seed Science and Technology 13, 69–76.Google Scholar
Ntuli, T.M., Finch-Savage, W.E., Berjak, P. and Pammenter, N.W. (2011) Increased drying rate lowers the critical water content for survival in embryonic axes of English oak (Quercus rober L.) seeds. Journal of Integrative Plant Biology 53, 270–280.CrossRefGoogle Scholar
Oliveira, L.M.Q. and Valio, I.F.M. (1992) Effects of moisture content on germination of seeds of Hancornia speciosa Gom. (Apocynaceae). Annals of Botany 69, 1–5.CrossRefGoogle Scholar
Osborne, D.J., Boubriak, I. and Leprince, O. (2002) Rehydration of dried systems: membranes and the nuclear genome. pp. 343–364 in Black, M.; Pritchard, H.W. (Eds) Desiccation and survival in plants. Drying without dying. Wallingford, CABI Publishing.CrossRefGoogle Scholar
Pammenter, N.W., Vertucci, C.W. and Berjak, P. (1991) Homeohydrous (recalcitrant) seeds: dehydration, the state of water and viability characteristics in Landolphia kirkii . Plant Physiology 96, 1093–1098.CrossRefGoogle ScholarPubMed
Pammenter, N.W., Greggains, V., Kioko, J.I., Wesley-Smith, J., Berjak, P. and Finch-Savage, W.E. (1998) Effects of differential drying rates on viability retention of recalcitrant seeds of Ekebergia capensis . Seed Science Research 8, 463–471.CrossRefGoogle Scholar
Pammenter, N.W., Berjak, P., Wesley-Smith, J. and Vander Willigen, C. (2002) Experimental aspects of drying and recovery. pp. 93–110 in Black, M.; Pritchard, H.W. (Eds) Desiccation and survival in plants: drying without dying. Wallingford, CABI Publishing.CrossRefGoogle Scholar
Panza, V., Lainez, V., Maldonado, S. and Marodor, H.L. (2007) Effects of desiccation on Euterpe edulis Martius seeds. BIOCELL 31, 383–390.CrossRefGoogle Scholar
Poulsen, K.M. and Eriksen, E.N. (1992) Physiological aspects of recalcitrance in embryonic axes of Quercus robur L. Seed Science Research 2, 215–221.CrossRefGoogle Scholar
Pukacka, S. (1989) The effect of desiccation on viability and phospholipid composition of Acer sacharinum L. seeds. Trees - Structure and Function 3, 144–148.CrossRefGoogle Scholar
Pukacka, S. and Ratajczak, E. (2006) Antioxidative response of ascorbate-glutathione pathway enzymes and metabolites to desiccation of recalcitrant Acer saccharinum seeds. Journal of Plant Physiology 163, 1259–1266.CrossRefGoogle ScholarPubMed
Raja, K. and Palanisamy, V. (2009) Physiology, vigour and viability of desiccation-sensitive arecanut (Areca catechu L.) seeds influenced by waxing. Seed Science and Technology 37, 457–463.CrossRefGoogle Scholar
Roach, T., Ivanova, M., Beckett, R.P., Minibayeva, F.V., Green, I., Pritchard, H.W. and Kranner, I. (2008) An oxidative burst of superoxide in embryonic axes of recalcitrant sweet chestnut seeds as induced by excision and desiccation. Physiologia Plantarum 133, 131–139.CrossRefGoogle ScholarPubMed
Salmen Espindola, L., Noin, M., Corbineau, F. and Côme, D. (1994) Cellular and metabolic damage induced by desiccation in recalcitrant Araucaria angustifolia embryos. Seed Science Research 4, 193–201.CrossRefGoogle Scholar
Shao, Y.T., Yin, S.H. and Wang, Z.J. (2009) Desiccation tolerance and cryopreservation of Archontophoenix alexandrae excised embryos at different developmental stages. Seed Science and Technology 37, 147–160.CrossRefGoogle Scholar
Simon, E.W. (1974) Phospholipids and plant membrane permeability. New Phytologist 73, 377–420.CrossRefGoogle Scholar
Song, S.-Q., Berjak, P., Pammenter, N., Ntuli, T.M. and Fu, J.-R. (2003) Seed recalcitrance: a current assessment. Acta Botanica Sinica 45, 638–643.Google Scholar
Song, S.Q., Berjak, P. and Pammenter, N. (2004) Desiccation sensitivity of Trichilia dregeana axes and antioxidant role of ascorbic acid. Acta Botanica Sinica 46, 803–810.Google Scholar
Sunilkumar, K.K. and Sudhakara, K. (1998) Effect of temperature, media, and fungicide on the storage behavior of Hopea parviflora seeds. Seed Science and Technology 26, 781–797.Google Scholar
Wesley-Smith, J., Pammenter, N.W., Berjak, P. and Walters, C. (2001) The effects of two drying rates on the desiccation tolerance of embryonic axes of recalcitrant jackfruit (Artocarpus heterophyllus Lamk.) seeds. Annals of Botany 88, 653–664.CrossRefGoogle Scholar
Whitaker, C., Beckett, R.P., Minibayeva, F.V. and Kranner, I. (2010) Production of reactive oxygen species in excised, desiccated and cryopreserved explants of Trichilia dregeana Sond. South African Journal of Botany 76, 112–118.CrossRefGoogle Scholar
Xin, X., Jing, X.-M., Liu, Y. and Song, S.-Q. (2010) Viability loss pattern under rapid dehydration of Antiaris toxicaria axes and its relation to oxidative damage. Journal of Integrative Plant Biology 52, 434–441.CrossRefGoogle ScholarPubMed