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Protein synthesis during osmopriming of leek (Allium porrum L.) seeds

Published online by Cambridge University Press:  19 September 2008

P. A. Davison  and
C. M. Bray
P. A. Davison
Affiliation:
Department of Biochemistry and Molecular Biology, University of Manchester, Oxford Road, Manchester M13 9PT, UK
C. M. Bray*
Affiliation:
Department of Biochemistry and Molecular Biology, University of Manchester, Oxford Road, Manchester M13 9PT, UK
*
* Correspondence
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Abstract

An osmotic priming treatment of 14 days in a −1.0 MPa polyethylene glycol solution improves the germination performance of a highvigour seed lot of leek (Allium porrum L., cv. Verina). Using in vivo pulse-labelling, two-dimensional polyacrylamide gel electrophoresis and fluorography, five polypeptides were found to be synthesized in embryonic tissue at 14 days of priming that were not present at 4 days of germination without prior priming. These polypeptides were still synthesized at 6 h of germination following priming. This time point lies in the 6–12 h lag phase in protein synthesis previously observed in leek embryo tissue during germination of primed seeds where there is little increase in the rate of synthesis over that seen at the end of priming. None of the polypeptides was synthesized at 2 days of germination after priming, a period of seedling growth. The five polypeptides appear to be specifically associated with the priming period. Two additional polypeptides were found in leek embryos that were synthesized at higher levels at the end of priming than during germination alone. These continued to be synthesized, at lower levels in leek embryo tissue upon germination after priming. Several polypeptides were identified in leek endosperm tissue which were synthesized at higher levels during priming than during germination and also two polypeptides whose synthesis appeared to be specific to germination.

Keywords

Allium porrum L.germinationleeksosmoprimingprotein synthesisseeds
Type
Research Papers
Information
Seed Science Research , Volume 1 , Issue 1 , March 1991 , pp. 29 - 35
Copyright
Copyright © Cambridge University Press 1991

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Footnotes

1

Current address: Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK

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