Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-15T15:20:55.565Z Has data issue: false hasContentIssue false
  • English
  • Français

Non-destructive X-ray analysis of Arabidopsis embryo mutants

Published online by Cambridge University Press:  19 September 2008

R. J. Bino ,
J. W. Aartse  and
W. J. van der Burg
R. J. Bino*
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
J. W. Aartse
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
W. J. van der Burg
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
*
* Correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

X-radiography is a simple, rapid and non-destructive method for analysing the morphology of embryos in dry, mature seeds of Arabidopsis thaliana. In wild type seeds, the cotyledons, hypocotyl and radicle tip can be readily distinguished. In seeds of the mutant types knolle, keule, and the double mutant keulelgnom, aberrations in embryo morphology can be visualized. X-radiography may therefore be useful in the isolation of embryo mutants from Arabidopsis seed samples.

Keywords

Arabidopsisembryo mutantsseedX-radiography
Type
Research Papers
Information
Seed Science Research , Volume 3 , Issue 3 , September 1993 , pp. 167 - 170
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Argerich, C.A. and Bradford, K.L. (1989) The effects of priming and ageing on seed vigour in tomato. Journal of Experimental Botany 40, 599–607.CrossRefGoogle Scholar
Goldberg, R.B., Barker, S.J. and Perez-Grau, L. (1989) Regulation of gene expression during plant embryogenesis. Cell 56, 149–160.Google Scholar
Jürgens, G., Mayer, U., Torres Ruiz, R.A., Berleth, T. and Miséra, S. (1991) Genetic analysis of pattern formation in the Arabidopsis embryo. pp 27–38in Roberts, K. (Ed.) Molecular and Cellular Basis of Pattern Formation, Cambridge, Company of Biologists, Ltd.Google Scholar
Mayer, U., Büttner, G. and Jürgens, G. (1993) Apical-basal pattern formation in the Arabidopsis embryo: studies on the role of the gnom gene. Development 117, 149–162.Google Scholar
Mayer, U., Torres Ruiz, R.A., Berleth, T., Miséra, S. and Jürgens, G. (1991) atMutations affecting body organization in the Arabidopsis embryo. Nature 353, 402–407.Google Scholar
Meinke, D.W. (1991) Perspectives on genetic analysis of plant embryogenesis. Plant Cell 3, 857–866.Google Scholar
Simak, M. (1991) Testing of forest tree and shrub seeds by X-radiography. pp 14(1)–14(28)in Gordon, A.G., Gosling, P. and Wang, B.S.P. (Eds) Tree and shrub seed handbook. Zurich, International Seed Testing Association.Google Scholar
Simak, M. and Gustafsson, A. (1953) X-ray photography and sensitivity in forest tree species. Hereditas 39, 458–468.Google Scholar