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Non-destructive X-ray analysis of Arabidopsis embryo mutants

Published online by Cambridge University Press:  19 September 2008

R. J. Bino ,
J. W. Aartse  and
W. J. van der Burg
R. J. Bino*
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
J. W. Aartse
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
W. J. van der Burg
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), POB 16, 6700 AA Wageningen, Netherlands
*
* Correspondence
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Abstract

X-radiography is a simple, rapid and non-destructive method for analysing the morphology of embryos in dry, mature seeds of Arabidopsis thaliana. In wild type seeds, the cotyledons, hypocotyl and radicle tip can be readily distinguished. In seeds of the mutant types knolle, keule, and the double mutant keulelgnom, aberrations in embryo morphology can be visualized. X-radiography may therefore be useful in the isolation of embryo mutants from Arabidopsis seed samples.

Keywords

Arabidopsisembryo mutantsseedX-radiography
Type
Research Papers
Information
Seed Science Research , Volume 3 , Issue 3 , September 1993 , pp. 167 - 170
Copyright
Copyright © Cambridge University Press 1993

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