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Membrane behaviour in seeds and other systems at low water content: the various effects of solutes

Published online by Cambridge University Press:  22 February 2007

Gary Bryant ,
Karen L. Koster  and
Joe Wolfe
Gary Bryant
Affiliation:
Department of Applied Physics, RMIT University, Melbourne 3001, Australia
Karen L. Koster*
Affiliation:
Department of Biology, The University of South Dakota, Vermillion, SD 57069, USA
Joe Wolfe
Affiliation:
School of Physics, The University of New South Wales, Sydney 2052, Australia
*
*Correspondence Fax: 1-605-677-6557 Email: [email protected]
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Abstract

A common feature of desiccation-tolerant organisms, such as orthodox seeds, is the presence of large quantities of sugars, especially di- and oligosaccharides. These sugars may be one component of the suite of adaptations that allow anhydrobiotes to survive the loss of most of their cellular water. This paper describes the physical effects of dehydration on cellular ultrastructure, with particular emphasis on membranes, and explains quantitatively how sugars and other solutes can influence these physical effects. As a result of dehydration, the surfaces of membranes are brought into close approach, which causes physical stresses that can lead to a variety of effects, including demixing of membrane components and fluid-to-gel phase transitions of membrane lipids. The presence of small solutes, such as sugars, between membranes can limit their close approach and, thereby, diminish the physical stresses that cause lipid fluid-to-gel phase transitions to occur during dehydration. Thus, in the presence of intermembrane sugars, the lipid fluid-to-gel phase transition temperature (Tm) does not increase as much as it does in the absence of sugars. Vitrification of the intermembrane sugar solution has the additional effect of adding a mechanical resistance to the lipid phase transition; therefore, when sugars vitrify between fluid phase bilayers, Tm is depressed below its fully hydrated value (To). These effects occur only for solutes small enough to remain in very narrow spaces between membranes at low hydration. Large solutes, such as polymers, may be excluded from such regions and, therefore, do not diminish the physical forces that lead to membrane changes at low hydration.

Keywords

dehydrationdesiccationmembranesphasetransitionsolutessugarsvitrification
Type
Research Perspective
Information
Seed Science Research , Volume 11 , Issue 1 , March 2001 , pp. 17 - 25
Copyright
Copyright © Cambridge University Press 2001

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