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Isolation and characterization of an expressed napin gene from Brassica rapa

Published online by Cambridge University Press:  19 September 2008

Jean C. Kridl ,
David W. McCarter ,
Ronald E. Rose ,
Donna E. Scherer ,
Deborah S. Knutzon ,
Sharon E. Radke  and
Vic C. Knauf
Jean C. Kridl*
Affiliation:
Calgene Inc., 1920 Fifth Street, Davis, CA 95616, USA
David W. McCarter
Affiliation:
Calgene Inc., 1920 Fifth Street, Davis, CA 95616, USA
Ronald E. Rose
Affiliation:
Calgene Inc., 1920 Fifth Street, Davis, CA 95616, USA
Donna E. Scherer
Affiliation:
Calgene Inc., 1920 Fifth Street, Davis, CA 95616, USA
Deborah S. Knutzon
Affiliation:
Calgene Inc., 1920 Fifth Street, Davis, CA 95616, USA
Sharon E. Radke
Affiliation:
Calgene Inc., 1920 Fifth Street, Davis, CA 95616, USA
Vic C. Knauf
Affiliation:
Calgene Inc., 1920 Fifth Street, Davis, CA 95616, USA
*
* Correspondence
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Abstract

An expressed napin storage protein gene from Brassica rapa, BcNA1, has been cloned and sequenced. The gene is a member of a family of four to seven napin genes in B. rapa and is highly expressed in developing seeds. An expression cassette containing the DNA flanking the napin coding region of BcNA1 has been engineered and demonstrated to function appropriately, as compared with the gene's endogenous expression, in transgenic rapeseed using the β-glucuronidase reporter gene. The B. rapa BcNA1 gene and a B. napus napin gene, gNa, share extremely high nucleotide homology not only throughout their coding regions, but over a DNA locus comprising 4.3 kb. We suggest the gNa gene was contributed by the original B. rapa progenitor of the amphidiploid B. napus.

Keywords

2S albuminsBrassica rapachimeric genesembryo-specific promotersstorage protein processingtransgenic rapeseed
Type
Research Papers
Information
Seed Science Research , Volume 1 , Issue 4 , December 1991 , pp. 209 - 219
Copyright
Copyright © Cambridge University Press 1991

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Footnotes

1

Current address: Vollum L-474, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201, USA

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