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Generation of low phytic acid Arabidopsis seeds expressing an E. coli phytase during embryo development

Published online by Cambridge University Press:  22 February 2007

Patricia Coello ,
Jeffrey P. Maughan ,
Alex Mendoza ,
Reena Philip ,
David W. Bollinger ,
Trygve L. Veum ,
Lila O. Vodkin  and
Joe C. Polacco
Patricia Coello*
Affiliation:
Departments of Biochemistr, 708 Quince Orchard Road, Gaithersburg, MD 20878, USA
Jeffrey P. Maughan
Affiliation:
Department of Monsanto Corporation, Ankeny, IA 50021708 Quince Orchard Road, Gaithersburg, MD 20878, USA
Alex Mendoza
Affiliation:
Departments of Biochemistr, 708 Quince Orchard Road, Gaithersburg, MD 20878, USA
Reena Philip
Affiliation:
Department of Crop Science, University of Illinois, Urbana, IL 61801708 Quince Orchard Road, Gaithersburg, MD 20878, USA Department of Gene Logic Inc., 708 Quince Orchard Road, Gaithersburg, MD 20878, USA
David W. Bollinger
Affiliation:
Departments of Animal Sciences, University of Missouri-Columbia, Columbia, MO 65211708 Quince Orchard Road, Gaithersburg, MD 20878, USA
Trygve L. Veum
Affiliation:
Departments of Animal Sciences, University of Missouri-Columbia, Columbia, MO 65211708 Quince Orchard Road, Gaithersburg, MD 20878, USA
Lila O. Vodkin
Affiliation:
Department of Crop Science, University of Illinois, Urbana, IL 61801708 Quince Orchard Road, Gaithersburg, MD 20878, USA
Joe C. Polacco
Affiliation:
Departments of Biochemistr, 708 Quince Orchard Road, Gaithersburg, MD 20878, USA
*
*Correspondence Fax: 5622 5329 Email: [email protected]
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Abstract

An Escherichia coli phytase gene was introduced into Arabidopsisplants using an embryo-specific promoter and a signal peptide for vacuolar targeting. Three independent transgenic lines were analysed. Phytase activity in dry seeds was observed in transgenic lines, whereas no activity was detected in control, untransformed seeds. Transgenic seeds expressing the phytase gene had lower levels of phytic acid than the controls. Concomitant with the decrease in phytic acid was an increase in free phosphate. These results indicated that embryo-expressed phytase can reduce the levels of phytic acid stored during development.

Keywords

Arabidopsisphytasephytic acidEscherichia coli
Type
Research Article
Information
Seed Science Research , Volume 11 , Issue 4 , December 2001 , pp. 285 - 291
Copyright
Copyright © Cambridge University Press 2001

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