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Fire-related cues and the germination of eight Conostylis (Haemodoraceae) taxa, when freshly collected, after burial and after laboratory storage

Published online by Cambridge University Press:  28 July 2015

Katherine S. Downes ,
Marnie E. Light ,
Martin Pošta  and
Johannes van Staden
Katherine S. Downes*
Affiliation:
Department of Environment and Agriculture, Curtin University, PO Box U1987, Perth, WA 6845, Australia
Marnie E. Light
Affiliation:
Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa
Martin Pošta
Affiliation:
Institute of Organic Chemistry and Biochemistry AS CR, v.v.i., Flemingovo nám. 2., 166 10, Prague 6, Czech Republic
Johannes van Staden
Affiliation:
Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa
*
*Correspondence E-mail: [email protected]
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Abstract

The genus Conostylis (Haemodoraceae) is endemic to fire-prone south-western Australia. To gain an understanding of the effect of some fire-related germination cues, eight Conostylis taxa were tested in response to water, nitrate, smoke water and karrikinolide (KAR1) under light and dark conditions, when seeds were freshly collected and after a year of burial. The germination of all taxa tested was higher in response to smoke water and KAR1 than in water alone, whereas nitrate did not stimulate germination. Germination was higher in all taxa following 1 year of burial than in fresh seeds. Recently, glyceronitrile has been identified as another chemical in smoke water, apart from KAR1, that can stimulate the germination of certain species. The relative response of eight Conostylis taxa to KAR1, glyceronitrile and smoke water was examined in laboratory-stored seeds. Germination of these taxa was promoted by both smoke water and KAR1, except for C. neocymosa, which had high germination regardless of treatment. Four of the other seven taxa germinated to higher levels in at least one of the glyceronitrile concentrations tested (10, 50 or 100 μM) than in water alone. However, in only two of these taxa, C. aculeata subsp. septentrionora and C. juncea, was germination in glyceronitrile as high as that in smoke water. Thus, the response to glyceronitrile is not uniform across Conostylis taxa. Generally, germination was higher with KAR1 than glyceronitrile, suggesting that although some Conostylis taxa have the capacity to respond to glyceronitrile, KAR1 is the more important germination stimulant for this genus.

Keywords

ConostylisgerminationglyceronitrileHaemodoraceaekarrikinolideseed dormancysmoke water
Type
Research Papers
Information
Seed Science Research , Volume 25 , Issue 3 , September 2015 , pp. 286 - 298
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Copyright
Copyright © Cambridge University Press 2015 

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References

Baker, K.S., Steadman, K.J., Plummer, J.A. and Dixon, K.W. (2005a) Seed dormancy and germination responses of nine Australian fire ephemerals. Plant and Soil 277, 345–358.CrossRefGoogle Scholar
Baker, K.S., Steadman, K.J., Plummer, J.A., Merritt, D.J. and Dixon, K.W. (2005b) The changing window of conditions that promotes germination of two fire ephemerals, Actinotus leucocephalus (Apiaceae) and Tersonia cyathiflora (Gyrostemonaceae). Annals of Botany 96, 1225–1236.Google Scholar
Baker, K.S., Steadman, K.J., Plummer, J.A., Merritt, D.J. and Dixon, K.W. (2005c) Dormancy release in Australian fire ephemeral seeds during burial increases germination response to smoke water or heat. Seed Science Research 15, 339–348.Google Scholar
Baldwin, I.T. and Morse, L. (1994) Up in smoke: II. Germination of Nicotiana attenuata in response to smoke-derived cues and nutrients in burned and unburned soils. Journal of Chemical Ecology 20, 2373–2391.Google Scholar
Baskin, C.C., Thompson, K. and Baskin, J.M. (2006) Mistakes in germination ecology and how to avoid them. Seed Science Research 16, 165–168.Google Scholar
Baskin, J.M. and Baskin, C.C. (2004) A classification system for seed dormancy. Seed Science Research 14, 1–16.Google Scholar
Bell, D.T. (1999) The process of germination in Australian species. Australian Journal of Botany 47, 475–517.Google Scholar
Bell, D.T. (2001) Ecological response syndromes in the flora of southwestern Western Australia: fire resprouters versus reseeders. The Botanical Review 67, 417–440.CrossRefGoogle Scholar
Bell, D.T., Vlahos, S. and Watson, L.E. (1987) Stimulation of seed germination of understorey species of the northern jarrah forest of Western Australia. Australian Journal of Botany 35, 593–599.CrossRefGoogle Scholar
Bellairs, S.M. and Bell, D.T. (1990) Temperature effects on the seed germination of ten kwongan species from Eneabba, Western Australia. Australian Journal of Botany 38, 451–458.CrossRefGoogle Scholar
Bouwmeester, H.J. and Karssen, C.M. (1993) Annual changes in dormancy and germination in seeds of Sisymbrium officinale (L.) Scop. New Phytologist 124, 179–191.CrossRefGoogle Scholar
Brown, N.A.C. and Botha, P.A. (2004) Smoke seed germination studies and a guide to seed propagation of plants from the major families of the Cape Floristic Region, South Africa. South African Journal of Botany 70, 559–581.Google Scholar
Brown, N.A.C., Van Staden, J., Daws, M.I. and Johnson, T. (2003) Patterns in the seed germination response to smoke in plants from the Cape Floristic region, South Africa. South African Journal of Botany 69, 514–525.Google Scholar
Çatav, Ş.S., Küçükakyüz, K., Akbaş, K. and Tavşanoğlu, Ç. (2014) Smoke-enhanced seed germination in Mediterranean Lamiaceae. Seed Science Research 24, 257–264.Google Scholar
Chiwocha, S.D.S., Dixon, K.W., Flematti, G.R., Ghisalberti, E.L., Merritt, D.J., Nelson, D.C., Riseborough, J.A., Smith, S.M. and Stevens, J.C. (2009) Karrikins: a new family of plant growth regulators in smoke. Plant Science 177, 252–256.Google Scholar
Christensen, N.L. (1973) Fire and the nitrogen cycle in Californian chaparral. Science 181, 66–68.CrossRefGoogle Scholar
Cowling, R.M., Rundel, P.W., Lamont, B.B., Arroyo, M.K. and Arianoutsou, M. (1996) Plant diversity in mediterranean-climate regions. Trends in Ecology and Evolution 11, 362–366.CrossRefGoogle ScholarPubMed
De Lange, J.H. and Boucher, C. (1990) Autecological studies on Audouinia capitata (Bruniaceae). 1. Plant-derived smoke as a seed germination cue. South African Journal of Botany 56, 700–703.CrossRefGoogle Scholar
Derkx, M.P.M. and Karssen, C.M. (1993) Changing sensitivity to light and nitrate but not to gibberellins regulates seasonal dormancy patterns in Sisymbrium officinale seeds. Plant, Cell and Environment 16, 469–479.CrossRefGoogle Scholar
Dixon, K.W., Roche, S. and Pate, J.S. (1995) The promotive effect of smoke derived from burnt native vegetation on seed germination of Western Australian plants. Oecologia 101, 185–192.CrossRefGoogle ScholarPubMed
Downes, K.S., Lamont, B.B., Light, M.E. and Van Staden, J. (2010) The fire ephemeral Tersonia cyathiflora (Gyrostemonaceae) germinates in response to smoke but not the butenolide, 3-methyl-2H-furo[2,3-c]pyran-2-one. Annals of Botany 106, 381–384.Google Scholar
Downes, K.S., Light, M.E., Pošta, M., Kohout, L. and Van Staden, J. (2013) Comparison of germination responses of Anigozanthos flavidus (Haemodoraceae), Gyrostemon racemiger and Gyrostemon ramulosus (Gyrostemonaceae) to smoke-water and the smoke-derived compounds karrikinolide (KAR1) and glyceronitrile. Annals of Botany 111, 489–497.Google Scholar
Downes, K.S., Light, M.E., Pošta, M., Kohout, L. and Van Staden, J. (2014) Do fire-related cues, including smoke-water, karrikinolide, glyceronitrile and nitrate, stimulate the germination of 17 Anigozanthos taxa and Blancoa canescens (Haemodoraceae)? Australian Journal of Botany 62, 347–358.Google Scholar
Drewes, F.E., Smith, M.T. and Van Staden, J. (1995) The effect of a plant-derived smoke extract on the germination of light-sensitive lettuce seed. Plant Growth Regulation 16, 205–209.Google Scholar
Flematti, G.R., Ghisalberti, E.L., Dixon, K.W. and Trengove, R.D. (2004) A compound from smoke that promotes seed germination. Science 305, 977.Google Scholar
Flematti, G.R., Ghisalberti, E.L., Dixon, K.W. and Trengove, R.D. (2005) Synthesis of the seed germination stimulant 3-methyl-2H-furo[2,3-c]pyran-2-one. Tetrahedron Letters 46, 5719–5721.CrossRefGoogle Scholar
Flematti, G.R., Ghisalberti, E.L., Dixon, K.W. and Trengove, R.D. (2009) Identification of alkyl substituted 2H-furo[2,3-c]pyran-2-ones as germination stimulants present in smoke. Journal of Agricultural and Food Chemistry 57, 9475–9480.Google Scholar
Flematti, G.R., Merritt, D.J., Piggott, M.J., Trengrove, R.D., Smith, S.M., Dixon, K.W. and Ghisalberti, E.L. (2011) Burning vegetation produces cyanohydrins that liberate cyanide and stimulate seed germination. Nature Communications 2, 360.Google Scholar
Flematti, G.R., Waters, M.T., Scaffidi, A., Merritt, D.J., Ghisalberti, E.L., Dixon, K.W. and Smith, S.M. (2013) Karrikin and cyanohydrin signals provide clues to new endogenous plant signaling compounds. Molecular Plant 6, 29–37.CrossRefGoogle ScholarPubMed
Hopper, S.D. (1993) Kangaroo paws and catspaws. Perth, Australia, Department of Conservation and Land Management.Google Scholar
Hopper, S.D. and Gioia, P. (2004) The southwest Australian floristic region: evolution and conservation of a global hot spot of biodiversity. Annual Review of Ecology and Systematics 35, 623–650.Google Scholar
Hopper, S.D., Chase, M.W. and Fay, M.F. (2006) A molecular phylogenetic study of generic and subgeneric relationships in the southwest Australian endemics Conostylis and Blancoa (Haemodoraceae). Aliso 22, 527–538.CrossRefGoogle Scholar
Hopper, S.D., Smith, R.J., Fay, M.F., Manning, J.C. and Chase, M.W. (2009) Molecular phylogenetics of Haemodoraceae in the Greater Cape and Southwest Australian Floristic Regions. Molecular Phylogenetics and Evolution 51, 19–30.CrossRefGoogle ScholarPubMed
Keeley, J.E. and Fotheringham, C.J. (1998a) Smoke-induced seed germination in California chaparral. Ecology 79, 2320–2336.Google Scholar
Keeley, J.E. and Fotheringham, C.J. (1998b) Mechanism of smoke-induced seed germination in a post-fire chaparral annual. Journal of Ecology 86, 27–36.Google Scholar
Kopecký, J. and Šmejkal, J. (1984) Einfache darstellung von acrylnitrilmetaboliten: oxirancarbonitril und 2,3-dihydroxy-propionitril. Zeitschrift für Chemie 24, 211–212.CrossRefGoogle Scholar
Lamont, B.B. and Downes, K.S. (2011) Fire-stimulated flowering among resprouters and geophytes in Australia and South Africa. Plant Ecology 212, 2111–2125.Google Scholar
Lloyd, M.V. (2001) An evaluation of the potential for smoke products to be used as a tool in the revegetation of broadscale areas. MSc thesis, The University of Western Australia, Perth.Google Scholar
Lloyd, M.V., Dixon, K.W. and Sivasithamparam, K. (2000) Comparative effects of different smoke treatments on germination of Australian native plants. Austral Ecology 25, 610–615.Google Scholar
Luna, B. and Moreno, J.M. (2009) Light and nitrate effects on seed germination of Mediterranean plant species of several functional groups. Plant Ecology 203, 123–135.Google Scholar
Macfarlane, T.D., Hopper, S.D., Purdie, R.W., George, A.S. and Patrick, S.J. (1987) Haemodoraceae. Flora of Australia 45, 55–147.Google Scholar
Merritt, D.J., Kristiansen, M., Flematti, G.R., Turner, S.R., Ghisalberti, E.L., Trengove, R.D. and Dixon, K.W. (2006) Effects of a butenolide present in smoke on light-mediated germination of Australian Asteraceae. Seed Science Research 16, 29–35.Google Scholar
Merritt, D.J., Turner, S.R., Clarke, S. and Dixon, K.W. (2007) Seed dormancy and germination syndromes for Australian temperate species. Australian Journal of Botany 55, 336–344.Google Scholar
Nelson, D.C., Riseborough, J.A., Flematti, G.R., Stevens, J., Ghisalberti, E.L., Dixon, K.W. and Smith, S.M. (2009) Karrikins discovered in smoke trigger Arabidopsis seed germination by a mechanism requiring gibberellic acid synthesis and light. Plant Physiology 149, 863–873.Google Scholar
Newton, R.J., Bond, W.J. and Farrant, J.M. (2006) Effects of seed storage and fire on germination in the nut-fruited Restionaceae species, Cannomois virgata . South African Journal of Botany 72, 177–180.Google Scholar
Norman, M.A., Plummer, J.A., Koch, J.M. and Mullins, G.R. (2006) Optimising smoke treatments for jarrah (Eucalyptus marginata) forest rehabilitation. Australian Journal of Botany 54, 571–581.Google Scholar
Ooi, M.K.J., Auld, T.D. and Whelan, R.J. (2006) Dormancy and the fire-centric focus: germination of three Leucopogon species (Ericaceae) from south-eastern Australia. Annals of Botany 98, 421–430.Google Scholar
Pate, J.S. and Hopper, S.D. (1993) Rare and common plants in ecosystems, with special reference to the south-west Australian flora. pp. 293–325 in Schulze, E.D.; Mooney, H.A. (Eds) Biodiversity and ecosystem function. Berlin, Springer-Verlag.Google Scholar
Pausas, J.G. and Keeley, J.E. (2009) A burning story: the role of fire in the history of life. BioScience 59, 593–601.CrossRefGoogle Scholar
Pérez-Fernández, M.A. and Rodríguez-Echeverría, S. (2003) Effect of smoke, charred wood, and nitrogenous compounds on seed germination of ten species from woodland in central-western Spain. Journal of Chemical Ecology 29, 237–251.Google Scholar
Plummer, J.A., Rogers, A.D. and Bell, D.T. (2001) Light, nitrogenous compounds, smoke and GA3 break dormancy and enhance germination in the Australian everlasting daisy, Shoenia filifolia subsp. subulifolia . Seed Science and Technology 29, 321–330.Google Scholar
Read, T.R., Bellairs, S.M., Mulligan, D.R. and Lamb, D. (2000) Smoke and heat effects on soil seed bank germination for the re-establishment of a native forest community in New South Wales. Austral Ecology 25, 48–57.Google Scholar
Roche, S., Dixon, K.W. and Pate, J.S. (1997) Seed ageing and smoke: partner cues in the amelioration of seed dormancy in selected Australian native species. Australian Journal of Botany 45, 783–815.Google Scholar
Steadman, K.J., Crawford, A.D. and Gallagher, R.S. (2003) Dormancy release in Lolium rigidum seeds is a function of thermal after-ripening time and seed water content. Functional Plant Biology 30, 345–352.Google Scholar
Stock, W.D. and Lewis, O.A.M. (1986) Soil nitrogen and the role of fire as a mineralizing agent in a South African coastal fynbos ecosystem. Journal of Ecology 74, 317–328.Google Scholar
Tacey, W.H. and Glossop, B.L. (1980) Assessment of topsoil handling techniques for rehabilitation of sites mined for bauxite within the jarrah forest of Western Australia. Journal of Applied Ecology 17, 195–201.Google Scholar
Thanos, C.A. and Rundel, P.W. (1995) Fire-followers in chaparral: nitrogenous compounds trigger seed germination. Journal of Ecology 83, 207–216.Google Scholar
Tieu, A., Dixon, K.W., Sivasithamparam, K., Plummer, J.A. and Sieler, I.M. (1999) Germination of four species of native Western Australian plants using plant-derived smoke. Australian Journal of Botany 47, 207–219.Google Scholar
Tieu, A., Dixon, K.W., Meney, K.A. and Sivasithamparam, K. (2001) Interaction of soil burial and smoke on germination patterns in seeds of selected Australian native plants. Seed Science Research 11, 69–76.Google Scholar
Turner, S.R., Merritt, D.J., Renton, M.S. and Dixon, K.W. (2009) Seed moisture content affects afterripening and smoke responsiveness in three sympatric Australian native species from fire-prone environments. Austral Ecology 34, 866–877.Google Scholar
Turner, S.R., Steadman, K.J., Vlahos, S., Koch, J.M. and Dixon, K.W. (2013) Seed treatment optimizes benefits of seed bank storage for restoration-ready seeds: the feasibility of prestorage dormancy alleviation for mine-site revegetation. Restoration Ecology 21, 186–192.Google Scholar
Tyler, C.M. (1995) Factors contributing to postfire seedling establishment in chaparral: direct and indirect effects of fire. Journal of Ecology 83, 1009–1020.Google Scholar
Van Staden, J., Jäger, A.K., Light, M.E. and Burger, B.V. (2004) Isolation of the major germination cue from plant-derived smoke. South African Journal of Botany 70, 654–659.CrossRefGoogle Scholar
Waters, M.T., Nelson, D.C., Scaffidi, A., Flematti, G.R., Sun, Y.K., Dixon, K.W. and Smith, S.M. (2012) Specialisation within the DWARF14 protein family confers distinct responses to karrikins and strigolactones in Arabidopsis . Development 139, 1285–1295.Google Scholar
Western Australian Herbarium . (1998–) FloraBase—the Western Australian Flora. Perth, Department of Parks and Wildlife. Available at http://florabase.dpaw.wa.gov.au/ (verified 11 January 2015). Google Scholar
Zar, J.H. (2014) Biostatistical analysis (5th edition). Harlow, UK, Pearson Education Limited.Google Scholar