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Environmentally induced polymorphisms detected by RAPD analysis of soybean seed DNA

Published online by Cambridge University Press:  19 September 2008

Robert G. Shatters Jr ,
Mark E. Schweder ,
S. H. West ,
Ashraf Abdelghany  and
Rex L. Smith
Robert G. Shatters Jr*
Affiliation:
USDA, ARS, Agronomy Dept., University of Florida, Gainesville, FL 32611, USA
Mark E. Schweder
Affiliation:
Agronomy Department, University of Florida, Gainesville, FL 32611, USA
S. H. West
Affiliation:
USDA, ARS, Agronomy Dept., University of Florida, Gainesville, FL 32611, USA
Ashraf Abdelghany
Affiliation:
Ain Shams University, Faculty of Agriculture, P.O. Box 68, Soubra El-Khema, Cairo, Egypt
Rex L. Smith
Affiliation:
Agronomy Department, University of Florida, Gainesville, FL 32611, USA
*
*Correspondence
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Abstract

Experiments were performed to determine if RAPD profiles developed using total DNA isolated from soybean seed could be affected by the physiological state or the quality of the seed. RAPD profiles were developed using template DNA isolated from a single seed lot of soybean (Glycine max L. cv. Kirby). High quality seeds were used to produce four populations varying in either quality or physiological state: untreated control seed ambient temperature and humidity storage for 12 months, accelerated aging at 41°C and 100% relative humidity for 48 h, and controlled hydration (seed priming). One hundred and eighty-eight primers were used to create separate RAPD profiles from total DNA isolated from each set of seed and from soybean leaf tissue. Sixteen polymorphisms from 14 primers were identified as a result of seed treatments. Six primers showed nine polymorphisms in RAPD profiles of ambient-stored seed DNA, while four and two primers produced polymorphisms in reactions using accelerated aged or primed-seed template DNA, respectively. Two primers showed a polymorphic fragment in vegetative DNA not observed in any of the seed DNA samples. Ten of the observed polymorphisms were due to the appearance of a DNA fragment in response to a specific seed treatment while six were the result of the treatment-induced loss of a DNA fragment. The six polymorphisms resulting from the loss of a major fragment were all due to ambient-temperature seed storage. Results were reproducibly obtained from multiple DNA isolations using three separate DNA isolation procedures involving either multiple seed or a single seed as the template source. Therefore, genetically identical seed can consistently display RAPD polymorphisms as a response to the environmental exposure.

Keywords

SoybeanRAPDseed qualityDNA fingerprinting
Type
Research Papers
Information
Seed Science Research , Volume 5 , Issue 2 , June 1995 , pp. 109 - 116
Copyright
Copyright © Cambridge University Press 1995

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