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Effect of abscisic acid and slow drying on soluble carbohydrate content in developing embryoids of carrot (Daucus carota L.) and alfalfa (Medicago sativa L.)

Published online by Cambridge University Press:  19 September 2008

F. A. A. Tetteroo ,
C. Bomal ,
F. A. Hoekstra  and
C. M. Karssen
F. A. A. Tetteroo
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands Royal Sluis, Westeinde 161, 1601 BM Enkhuizen, Netherlands
C. Bomal
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
F. A. Hoekstra*
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
C. M. Karssen
Affiliation:
Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
*
* Correspondence
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Abstract

In the presence of abscisic acid (ABA), contents of glucose and fructose decreased in carrot embryoids, whereas umbelliferose increased during dehydration. The acquisition of desiccation tolerance was tested by scoring germinated embryoids. It did not keep pace with the changes in soluble carbohydrate contents during development, which suggests that in these somatic embryoids di- and oligosaccharides are not the determining factors for anhydrobiosis. However, on slow dehydration, umbelliferose increased, while sucrose decreased and the monosaccharides declined completely. These changes were positively correlated with increased desiccation tolerance. A similar analysis of slowly dried, ABA-treated alfalfa embryoids showed that stachyose and sucrose increased and the monosaccharides declined with the acquisition of desiccation tolerance. These data support the contention that carbohydrates are involved in anhydrobiosis.

Keywords

abscisic acidcarbohydratesDaucus carota L.desiccation toleranceMedicago sativa L.slow dryingsomatic embryogenesis
Type
Short Communication
Information
Seed Science Research , Volume 4 , Issue 2 , June 1994 , pp. 203 - 210
Copyright
Copyright © Cambridge University Press 1994

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References

Amuti, K.S. and Pollard, C.J. (1977) Soluble carbohydrates of dry and developing seeds. Phytochemistry 16, 529–532.CrossRefGoogle Scholar
Attree, S.M., Moore, D., Sawhney, V.K. and Fowke, L.C. (1991) Enhanced maturation and desiccation tolerance of white spruce (Picea glauca [Moench] Voss) somatic embryos: effects of a non-plasmolysing water stress and abscisic acid. Annals of Botany 68, 519–525.CrossRefGoogle Scholar
Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1992) Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. Plant Physiology 100, 225–230.CrossRefGoogle ScholarPubMed
Bruni, F. and Leopold, A.C. (1991) Glass transitions in soybean seeds. Plant Physiology 96, 660–663.CrossRefGoogle Scholar
Carpenter, J.F., Crowe, L.M. and Crowe, J.H. (1987) Stabilization of phosphofructokinase with sugars during freeze drying: characterization of enhanced protection in the presence of divalent cations. Biochimica et Biophysica Acta 406, 187–196.Google Scholar
Crowe, J.H., Crowe, L.M., Carpenter, J.F. and Aurell Wistrom, C. (1987) Stabilization of dry phospholipid bilayers and proteins by sugars. Biochemical Journal 242, 1–10.CrossRefGoogle ScholarPubMed
Crowe, J.H., Crowe, L.M. and Hoekstra, F.A. (1989) Phase transitions and permeability changes in dry membranes during rehydration. Journal of Bioenergetics and Biomembranes 21, 77–91.CrossRefGoogle ScholarPubMed