Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-29T10:06:58.714Z Has data issue: false hasContentIssue false
  • English
  • Français

The discovery of compositional variation for the raffinose family of oligosaccharides in pea seeds

Published online by Cambridge University Press:  22 February 2007

D. A. Jones ,
M. S. DuPont ,
M. J. Ambrose ,
J. Frias  and
C. L. Hedley
D. A. Jones
Affiliation:
Department of Applied Genetics, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK
M. S. DuPont
Affiliation:
Department of Diet, Health & Consumer Science, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK
M. J. Ambrose
Affiliation:
Department of Applied Genetics, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK
J. Frias
Affiliation:
Instituto de Fermentaciones Industriales CSIC, calle Juan de la Crierve 3, Madrid, Spain
C. L. Hedley
Affiliation:
Department of Applied Genetics, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

The raffinose family of oligosaccharides (RFO) is present in relatively high levels in grain legume seeds. They are considered to be antinutritional compounds because they are, at least in part, believed to be responsible for causing flatulence in humans, which is the single most important factor in deterring people from including more legume seeds in their diet. The RFO also have important functions within the plant. They serve as transport metabolites in many legumes and have been proposed to play a positive role in cold acclimatisation and in conferring desiccation tolerance during seed maturation. These responses to environmental stresses are believed to result from the RFO acting as protecting agents for membrane bound-proteins. We have screened 70 pea lines from the test array of the John Innes Pisum germplasm collection, and lines were selected which had unusual RFO composition. The soluble sugars within these lines were quantified using High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD), and variants were identified which were deficient in verbascose and one with a reduced level of raffinose. These selected lines are being used in a crossing programme designed to study the genetics of the RFO pathway and to produce new material to test the effect of specific RFO on the plant’s responses to the environment and on the diets of humans and animals.

Keywords

α-galactosidesgenetic variationpeaPisum sativumraffinose family of oligosaccharidesseed composition.
Type
Research Article
Information
Seed Science Research , Volume 9 , Issue 4 , April 1999 , pp. 305 - 310
Copyright
Copyright © Cambridge University Press 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ambrose, M.J. (1992) Making use of exotic germplasm: improvind the odds. Proceedings 1st European Conference on Grain Legumes. p. 39. Paris, European Association for Grain Legume Research.Google Scholar
Anonymous. (1989) Analysis of carbohydrates by anion exchange chromatography with pulsed amperometric detection. Dionex Technical Note 20, Sunnyvale, California, Dionex.Google Scholar
Bachmann, M., Matile, P. and Keller, F. (1994) Metabolism of the raffinose family oligosaccharides in leaves of Ajuga reptans L. Plant Physiology 105, 1335–1345.CrossRefGoogle ScholarPubMed
Bachmann, M., Inan, C. and Keller, F. (1995) Raffinose oligosaccharide storage. pp. 215–225in Madore, M.A.; Lucas, W.J. (Eds) Carbon partitioning and source-sink interactions in plants. Rockville, American Society of Plant Physiologists.Google Scholar
Bell, D.J. (1955) Mono- and oligosaccharides and acidic monosaccharide derivatives. Modern Methods of Plant Analysis 2, 1–54.Google Scholar
Bernal-Lugo, I. and Leopold, A.C. (1992) Changes in soluble carbohydrates during seed storage. Plant Physiology 98, 1207–1210.CrossRefGoogle ScholarPubMed
Castillo, E.M., de Lumen, B.O., Reyes, P.S. and de Lumen, H.Z. (1990) Raffinose synthase and galactinol synthase in developing seeds and leaves of legumes. Journal of Agricultural and Food Chemistry 38, 351–355.CrossRefGoogle Scholar
Cristofaro, E., Muttu, F. and Wuhrmann, J.J. (1974) Involvement of the raffinose family of oligosaccharides in flatulence. pp. 313–336in Sipple, H.L.; McNutt, K.W. (Eds) Sugars in nutrition. London, Academic Press.Google Scholar
Dey, P.M. (1980) Biochemistry of a-galactosidic linkages in the plant kingdom. Advances in Carbohydrate Chemistry and Biochemistry 37, 283–372.CrossRefGoogle Scholar
Dey, P.M. (1990) Oligosaccharides. pp. 189–218in Dey, P.M.; Harborne, J.B. (Eds) Methods in plant biochemistry, Vol. 2, Carbohydrates. London, Academic Press.Google Scholar
Ellis, T.H.N., Poyser, S.J., Knox, M.R., Vershinin, A.V. and Ambrose, M.J. (1998) Polymorphism of insertion sites of Ty1-copia class retrotransposons and its use for linkage and diversity analysis in pea. Molecular and General Genetics 260, 9–19.Google ScholarPubMed
Frias, J., Hedley, C.L., Price, K.R., Fenwick, G.R. and Vidal- Valverde, C. (1994a) Improved methods of oligosaccharide analysis for genetic studies of legume seeds. Journal of Liquid Chromatography 17, 2469–2483.CrossRefGoogle Scholar
Frias, J., Vidal-Valverde, C., Bakhsh, A., Arthur, A.E. and Hedley, C.L. (1994b) An assessment of variation for nutritional and non-nutritional carbohydrates in lentil seeds (Lens culinaris). Plant Breeding 113, 170–173.CrossRefGoogle Scholar
Frias, J., Price, K.R., Fenwick, G.R., Hedley, C.L., Sørensen, H. and Vidal-Valverde, C. (1996) Improved method for the analysis of a-galactosides in pea seeds by capillary zone electrophoresis. Comparison with high-performance liquid chromatography-triple-pulsed amperometric detection. Journal of Chromatography A 719, 213–219.CrossRefGoogle Scholar
Frias, J., Bakhsh, A., Jones, D.A., Arthur, A.E., Vidal- Valverde, C., Rhodes, M.J.C. and Hedley, C.L. (1999) Genetic analysis of the raffinose oligosaccharide pathway in lentil seeds. Journal of Experimental Botany 50, 469–476.CrossRefGoogle Scholar
Hedley, C.L., Smith, C.M., Ambrose, M.J., Cook, S. and Wang, T.L. (1986) An analysis of seed development in Pisum sativum L. II The effect of the r-locus on the growth and development of the seed. Annals of Botany 58, 371–379.CrossRefGoogle Scholar
Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: Dependence on flatulenceproducing oligosaccharides and cyclitols - review and survey. Seed Science Research 4, 385–405.CrossRefGoogle Scholar
Jones, D.A., Barber, L.M., Arthur, A.E. and Hedley, C.L. (1995) An analysis of seed development in Pisum sativum L. XVI. Assessing variation for fatty-acid content by use of a non-destructive technique for single seed analysis. Plant Breeding 114, 81–83.CrossRefGoogle Scholar
Kerr, P.S. (1993) Soybean products with improved carbohydrate composition and soybean plants. International Patent Publication Number WO 93/07742, 29 April 1993, PCT/US92/08958.Google Scholar
Kerr, P.S., Pearlstein, R.W., Becker-Manley, M.F. and Pierce, J.W. (1993) Nucleotide sequences of galactinol synthase from zucchini and soybean. International Patent Publication Number WO 93/02196, 4 February 1993, PCT/US92/06057.Google Scholar
Obendorf, R.L. (1997) Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance. Seed Science Research 7, 63–74.CrossRefGoogle Scholar
Price, K.R. (1986) A simple method for the extraction and isolation of verbascose from pea. Chemistry and Industry 3, 180–181.Google Scholar
Price, K.R., Lewis, J., Wyatt, G.M. and Fenwick, G.R. (1988) Flatulence - causes, relation to diet and remedies. Die Nährung 32, 609–626.CrossRefGoogle ScholarPubMed
Reddy, N.R., Pierson, M.D., Sate, S.K. and Salunkhe, D.K. (1984) Chemical, nutritional and physiological aspects of dry bean carbohydrates - A review. Food Chemistry 13, 25–68.CrossRefGoogle Scholar
Richards, E.A., Steggerda, F.R. and Murata, A. (1968) Relationship of bean substrates and certain intestinal bacteria to gas production in dog. Gastroenterology 55, 502–509.CrossRefGoogle ScholarPubMed
Salunkhe, D.K. and Kadam, S.S. (1989) Handbook of world legumes: Nutritional chemistry, processing technology, and utilization. Boca Raton, Florida, CRC Press Inc.Google Scholar
Stahl, E. (1969) Thin layer chromatography. Stahl, E. (Ed.). Berlin, Springer-Verlag.CrossRefGoogle ScholarPubMed