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Development of the strophiole in seeds of white clover (Trifolium repens L.)

Published online by Cambridge University Press:  19 September 2008

Helle Martens ,
Henrik B. Jakobsen  and
Ole B. Lyshede
Helle Martens*
Affiliation:
Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
Henrik B. Jakobsen
Affiliation:
Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
Ole B. Lyshede
Affiliation:
Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
*
*Correspondence
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Abstract

The seed coat of Trifolium repens L. was studied with special emphasis on the development of the strophiole, which is the site for water entry during imbibition in leguminous seeds. The epidermal cells of the strophiole are longer than the cells in the remainder of the seed epidermis in the mature ovule. During seed development the median cells of the strophiolar epidermis divide periclinally into an outer layer of palisade cells and an inner layer of isodiametric cells. Prior to maturity a fissure is formed between some of the palisade cells in the centre of the strophiole. It is suggested that tension develops between the palisade cells and the iso-diametrical cells during later maturation stages causing the formation of the fissure which it is believed functions in water uptake. It is indicated that the ‘light line’ is caused by alteration of cellulose microfibrillar orientation in palisade cell walls. It is confirmed that removal of the epicuticular wax from hard seeds by rinsing in absolute alcohol or hexane does not induce water imbibition. Only when seed coats are mechanically abraded do hard seeds germinate.

Keywords

Germinationhard seedlight lineseed coat anatomystrophioleTrifolium repenswax
Type
Development
Information
Seed Science Research , Volume 5 , Issue 3 , September 1995 , pp. 171 - 176
Copyright
Copyright © Cambridge University Press 1995

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Footnotes

1

Present address: Department of Food Science and Technology, Danish Institute of Plant and Soil Science, Kirstinebjergvej 12, 5792 Aarslev, Denmark

References

Corner, E.J.H. (1976) The seeds of dicotyledons I–II. 863 pp. Cambridge, Cambridge University Press.Google Scholar
Dell, B. (1980) Structure and function of the strophiolar plug in seeds of Albizia lophantha. American Journal of Botany 67, 556–563.CrossRefGoogle Scholar
Egley, G.H. (1989) Water-impermeable seed coverings as barriers to germination. NATO ASI series A, Life Sciences, Vol. 187. Recent Advances in the development and germination of seeds, New York, Plenum Press.Google Scholar
Hagon, M.W. and Ballard, L.A.T. (1970) Reversibility of strophiolar permeability to water in seeds of subterranean clover (Trifolium subterraneum L.) Australian Journal of Biological Science 23, 519–528.CrossRefGoogle Scholar
Hamly, D.H. (1932) Softening of the seeds of Melilotus alba. Botanical Gazette 93, 345–375.CrossRefGoogle Scholar
Hanna, P.J. (1984) Anatomical features of the seed coat of Acacia kempeana (Mueller) which relate to increased germination rate induced by heat treatment. New Phytologist 96, 23–29.CrossRefGoogle Scholar
Harris, W.M. (1983) On the development of macrosclereids in seed coats of Pisum sativum L. American Journal of Botany 70, 1528–1535.CrossRefGoogle Scholar
Hughes, J.S. and Swanson, B.G. (1986) Microstructure of lentil seeds (Lens culinaris). Food Microstructure 5, 241–246.Google Scholar
Jakobsen, H.B., Martens, H. and Lyshede, O.B. (1994) Metabolite translocation and accumulation during seed development in Trifolium repens L. Annals of Botany 74, 409–415.CrossRefGoogle Scholar
Lyshede, O.B. (1992) Studies on mature seeds of Cuscuta pedicellata and C. campestris by electron microscopy. Annals of Botany 69, 365–371.CrossRefGoogle Scholar
Manning, J.C. and van Staden, J. (1985) The development and ultrastructure of the testa and tracheid bar in Erythrina lysistemon Hutch. (Leguminosae: Papilionideae). Protoplasma 129, 157–167.CrossRefGoogle Scholar
Manning, J.C. and van Staden, J. (1987) The functional differentiation of the testa in seed of Indigofera parviflora (Leguminosae: Papilionideae). Botanical Gazette 148, 23–34.Google Scholar
Mattirolo, O. and Buscalioni, L. (1892) Ricerche anatomofisiologische sui tegumenti seminali delle Papilionacee. Memorie della Accademia delle Scienze di Torino 42, 223–318; 359–428.Google Scholar
Miklas, P.N., Townsend, C.E. and Ladd, S.L. (1987) Seed coat anatomy and the scarification of cicer milkvetch seed. Crop Science 27, 766–772.CrossRefGoogle Scholar
Priestly, D.A. (1986) Seed aging: implications for seed storage and persistence in the soil. New York, Ithaca, Cornell University Press.Google Scholar
Pritchard, H.W., Manger, K.R. and Prendergast, F.G. (1988) Changes in Trifolium arvense seed quality following alternating temperature treatment using liquid nitrogen. Annals of Botany 62, 1–11.CrossRefGoogle Scholar
Richards, D. and Beardsell, D. (1987) Seed dormancy, pp 1–13 in Langkamp, P. (Ed.) Germination of Australian native plant seed. Melbourne and Sydney, Inkata Press.Google Scholar
Rolston, M.P. (1978) Water impermeable seed dormancy. Botanical Review 44, 365–396.CrossRefGoogle Scholar
Werker, E. (1980/1981) Seed dormancy as explained by the anatomy of embryo envelopes. Israel Journal of Botany 29, 22–44.Google Scholar
Zimmermann, K. (1937) Zur physiologischen Anatomie der Leguminosen-testa. Beiträge zum Problem der Hartschaligkeit und zur Bedeutung des Strophiolums. Landwirtschaftliche Versuchsstationen 127, 1–56.Google Scholar