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Cyclitol galactosides in low-raffinose, low-stachyose soybean embryos after feeding d-chiro-inositol, myo-inositol or d-pinitol

Published online by Cambridge University Press:  25 February 2013

Ralph L. Obendorf ,
Elizabeth M. Sensenig ,
Erin M. Byrt ,
Anna B. Owczarczyk ,
Minori Ohashi  and
Steven R. Schnebly
Ralph L. Obendorf
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University Agricultural Experiment Station, 617 Bradfield Hall, Cornell University, Ithaca, NY 14,853-1901, USA
Elizabeth M. Sensenig
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University Agricultural Experiment Station, 617 Bradfield Hall, Cornell University, Ithaca, NY 14,853-1901, USA
Erin M. Byrt
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University Agricultural Experiment Station, 617 Bradfield Hall, Cornell University, Ithaca, NY 14,853-1901, USA
Anna B. Owczarczyk
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University Agricultural Experiment Station, 617 Bradfield Hall, Cornell University, Ithaca, NY 14,853-1901, USA
Minori Ohashi
Affiliation:
Seed Biology, Department of Crop and Soil Sciences, Cornell University Agricultural Experiment Station, 617 Bradfield Hall, Cornell University, Ithaca, NY 14,853-1901, USA
Steven R. Schnebly*
Affiliation:
Pioneer Hi-Bred, A DuPont Business, 810 Sugar Grove Ave., Hwy44, Dallas Center, IA 50063, USA
*
*Correspondence Fax: +1 607 255 2644 E-mail: [email protected]
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Abstract

Sucrose, raffinose and stachyose accumulate as stored soluble carbohydrates in embryos during soybean [Glycine max L. (Merrill)] seed development and maturation. Raffinose and stachyose in soybean feed products are not digested by humans, chickens or pigs, resulting in flatulence and reduced nutritional value. Soybean lines selected for low raffinose and low stachyose (LRS) or low raffinose, low stachyose and low phytin (LRSP1, LRSP2) concentrations in mature seeds were compared to a CHECK line with normal raffinose, stachyose and phytin. To determine whether increasing the supply of free cyclitols to immature embryos of these lines results in increased accumulation of galactosyl cyclitols, isolated immature embryos free of maternal tissues were fed solutions containing either d-chiro-inositol, myo-inositol or d-pinitol, or a control solution without cyclitols, for 24 h. Embryos were precociously matured by slow drying for 14 d with daily transfers to stepwise lower relative humidities. Soluble carbohydrates were extracted from axis and cotyledon tissues of mature, dry embryos and analysed by high-resolution gas chromatography. Axis and cotyledons from LRS, LRSP1 and LRSP2 embryos had low concentrations of stachyose compared to CHECK embryos after feeding a control solution without cyclitols. Feeding d-chiro-inositol to isolated embryos increased fagopyritol B1 accumulation in embryos of all lines. Feeding myo-inositol increased stachyose accumulation in LRSP1 and LRSP2 cotyledons. Feeding d-pinitol increased free d-pinitol in cotyledons of all lines but increased galactopinitol A and galactopinitol B only in LRS cotyledons. Supplying additional d-chiro-inositol to immature embryos can enhance accumulation of fagopyritol B1 in mature embryos of low-raffinose and low-stachyose or low-raffinose, low-stachyose and low-phytin soybeans.

Keywords

fagopyritolgalactopinitolGlycine maxd-chiro-inositold-pinitolsoybean embryo
Type
Research Papers
Information
Seed Science Research , Volume 23 , Issue 2 , June 2013 , pp. 111 - 122
Copyright
Copyright © Cambridge University Press 2013 

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