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Comparative biochemical and morphological changes in imbibed cotton seed hypocotyls and radicles in situ and in vitro – Protein breakdown and elongation growth

Published online by Cambridge University Press:  19 September 2008

Eugene L. Vigil  and
Tung K. Fang
Eugene L. Vigil*
Affiliation:
Climate Stress Laboratory, USDA/ARS, Bld 046A, BARC-West, Beltsville, MD 20705, USA
Tung K. Fang
Affiliation:
Climate Stress Laboratory, USDA/ARS, Bld 046A, BARC-West, Beltsville, MD 20705, USA
*
*Correspondence
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Abstract

Axes, hypocotyls and radicles excised from dry cotton seeds (Gossypium hirsutum L. cv. M-8, a double haploid) were imbibed for 24 h and compared with axial segments (excised sections of embryos below the cotyledons) of imbibed, intact seeds. Radicles of excised axes had a 7.4-fold increase in length compared with only 5.2- and 5.7-fold increases, respectively, in radicles of intact seeds and in those isolated when dry. Change in hypocotyl length was not as extensive. EM data for hypocotyl and radicle cortical cells from dry and imbibed seeds revealed a major reduction in matrix protein in protein storage vacuoles along with significant organelle development at 24 h from the start of imbibition. This occurred in parallel with a reduction in salt-extracted proteins and an increase in 2% SDS-extractable proteins. SDS-PAGE of protein from low (0.2 M NaCI) and high (1.0 M NaCI) salt extracts showed a reduction in amount of the major storage proteins (53 and 48 kDa), these bands being almost totally absent in gels of protein extracts from imbibed radicles and significantly reduced in hypocotyls, within 24 h from the start of imbibition. These results indicate that initial elongation of hypocotyls and radicles in intact seeds or of excised axes, after 24 h imbibition, involves breakdown of storage proteins in these axial parts to supply nutrients for growth, with very limited contribution from the cotyledons.

Keywords

CottonGossypium hirsutum L.hypocotylin vitro germinationradicleprotein storage vacuolesSDS-PAGE
Type
Research Papers
Information
Seed Science Research , Volume 5 , Issue 1 , March 1995 , pp. 41 - 51
Copyright
Copyright © Cambridge University Press 1995

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References

Bain, J.M. and Mercer, F.V. (1966a) Subcellular organization of the cotyledons in germinating seeds and seedlings of Pisum sativum L. Australian Journal of Biological Science 19, 69–84.CrossRefGoogle Scholar
Bain, J.M. and Mercer, F.V. (1966b) The relationship of the axis and the cotyledons in germinating seeds and seedlings of Pisum sativum L. Australian Journal of Biological Science 19, 85–96.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1982) Seeds. Physiology of development and germination. New York, London, Plenum Press.Google Scholar
Boylan, M.T. and Sussex, I.M. (1987) Purification of an endopeptidase involved with storage-protein degradation in Phaseolus vulgaris L. cotyledons. Planta 170, 343–352.CrossRefGoogle ScholarPubMed
Davies, H.V. and Chapman, J.M. (1979) The control of food mobilization in seeds of Cucumis sativus L. II. The role of the embryonic axis. Planta 146, 585–590.CrossRefGoogle ScholarPubMed
Dieckert, J.W., Wallace, R.W. and Dieckert, M.C. (1986) Chemistry and biology of the cotton seed globulins. pp 415–424in Mauney, J.R. and Stewart, J.M. (Eds) Cotton physiology. Memphis, TN, The Cotton Foundation Publishers.Google Scholar
Dunaevsky, Y.E. and Belozersky, M.A. (1993) Effects of the embryonic axis and phytohormones on proteolysis of the storage protein in buckwheat seed. Physiologia Plantarum 88, 60–64.CrossRefGoogle Scholar
Dure, L. III and Chlan, C.A. (1981) Developmental biochemistry of cottonseed embryogenesis and germination. XII. Purification and properties of principal storage proteins. Plant Physiology 68, 180–186.CrossRefGoogle ScholarPubMed
Dure, L. III and Chlan, C.A. (1985) Cotton seed storage proteins: Products of three gene families. pp 67–69in Van Vloten-Doting, L., Groot, G.S. and Hall, T.C. (Eds). Molecular form and function of the plant genome. New York, Plenum Press.Google Scholar
Dure, L.S. III, Galau, G.A. and Greenway, S. (1980/1981) changing protein patterns during cotton cotyledon embryogenesis and germination as shown by in vivo and in vitro synthesis. Israel Journal of Botany 29, 293–306.Google Scholar
Garcia-Agustin, P., Gastaldo, M.J.B. and Primo-Millo, E. (1991) Control by the embryo axis of the breakdown of storage proteins in cotyledons of germinating seeds of Citrus limon. Journal of the Science of Food and Agriculture 56, 435–443.CrossRefGoogle Scholar
Guardiola, J.L. and Sutcliffe, J.F. (1971) Control of protein hydrolysis in cotyledons of germinating pea (Pisum sativum L.) seeds. Annals of Botany 35, 791–807.CrossRefGoogle Scholar
Ihle, J.N. and Dure, L. III. (1972) The developmental biochemistry of cottonseed embryogenesis and germination. III. Regulation of biosynthesis of enzymes utilized in germination. Journal of Biological Chemistry 247, 5048–5055.CrossRefGoogle Scholar
Khademi, M., Koranski, D.S., Hannaple, D.J., Gladon, R.J., and Burris, J.S. (1991) Storage-protein degradation and aminopeptidase activity during germination of stressed and nonstressed Impatiens (Impatiens wallerana) seeds. Seed Science and Technology 19, 403–412.Google Scholar
King, E.E. (1980) Compositional relationships among electrophoretic isolates from cotton seed protein storage vacuoles. Phytochemistry 19, 1647–1651.CrossRefGoogle Scholar
Krochko, J.E. and Bewley, J.D. (1988) Use of electrophoretic techniques in determining the composition and seed storage proteins in alfalfa. Electrophoresis 9, 751–763.CrossRefGoogle ScholarPubMed
Laemmli, U.K. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680–682.CrossRefGoogle ScholarPubMed
Long, S.R., Dale, R.M.K. and Sussex, I.M. (1981) Maturation and germination of Phaseolus vulgaris embryonic axes in culture. Planta 153, 405–415.CrossRefGoogle ScholarPubMed
Markwell, M.A., Haas, S.M., Tolbert, N.E. and Bieber, L.L. (1981) Protein determination in membrane and lipoprotein samples: Manual and automated procedures. Methods in Enzymology 72, 296–303.CrossRefGoogle ScholarPubMed
Mitsuhashi, W., Koshiba, T. and Minamikawa, T. (1984) Influence of axes removal on amino-carboxy- and endopeptidase activity in cotyledons of germinating Vigna mungo seeds. Plant and Cell Physiology 25, 547–554.Google Scholar
Murray, D.R. (1979) Proteolysis in the axis of the germinating pea seed. II. Changes in polypeptide composition. Planta 47, 117–121.CrossRefGoogle Scholar
Murray, D.R. (1984) Seed Physiology Vol. 2. pp 247–279. Sydney, Academic Press.Google Scholar
Murray, D.R., Peoples, M.B. and Waters, S.P. (1979) Proteolysis in the axis of the germinating pea seed. I. Changes in protein degrading enzyme activities of the radicle and primary root. Planta 147, 111–116.CrossRefGoogle ScholarPubMed
Perner, E. (1965) Electronenmikroskopische Untersuchungen an Zellen von Embryonen in Zustand volliger Samenruh. II. Die Aleuronkorner in der Radicula lufttrockner Samen von Pisum sativum L. Planta 67, 324–343.CrossRefGoogle Scholar
Varner, J.E., Balce, L.V. and Huang, R.C. (1963) Senescence of cotyledons of germinating peas. Influence of axis tissue. Plant Physiology 38, 89–92.CrossRefGoogle ScholarPubMed
Vidovic, M. and Murray, D.R. (1984) Mobilization of nitrogen during imbibition and germination of seeds of Phaseolus vulgaris and Vigna unguiculata. Zeitschrift f¨r Pflanzenphysiologie 113, 117–128.CrossRefGoogle Scholar
Vigil, E.L. and Fang, T.K. (1993) Utilization of storage proteins by excised radicle/hypocotyls during in vitro germination. pp 357–362 in Côme, D., Corbinean, F. (Eds). Fourth international workshop on seeds, Vol. 2, Paris, ASFIS.Google Scholar
Vigil, E.L., Steere, R.L., Wergin, W.P. and Christiansen, M.N. (1984) Tissue preparation and fine structure of the radicle apex from cotton seeds. American Journal of Botany 71, 645–659.CrossRefGoogle Scholar
Vigil, E.L., Steere, R.L., Christiansen, M.N. and Erbe, E. (1985) Structural changes in protein storage vacuoles of cotton radicles during seed maturation and germination. pp 311–334in Robards, A.W. (Ed.) Botanical Microscopy. Oxford, Oxford University Press.Google Scholar
Walton, D.C. (1966) Germination of Phaseolus vulgaris I. Resumption of axis growth. Plant Physiology 41, 298–302.CrossRefGoogle ScholarPubMed
Wallace, R.W. (1976) Isolation and partial characterization of the acalin A and B fractions of cotton seed globulins. Dissertation. Texas A & M University. College Station, TX.Google Scholar
Waters, L.C. and Dure, L.S. III. (1966) Ribonucleic acid synthesis in germinating cotton seeds. Journal of Molecular Biology 19, 1–27.CrossRefGoogle ScholarPubMed
Wiley, L., and Ashton, E.M. (1963) Influence of embryonic axis on protein hydrolysis in cotyledons of Cucurbita maxima. Physiologia Plantarum 20, 688–696.CrossRefGoogle Scholar
Yomo, H. and Varner, J.E. (1973) Protein breakdown and formation of protease in attached and detached cotyledons of Phaseolus vulgaris L. Plant Physiology 52, 671–673.CrossRefGoogle ScholarPubMed
Yoo, B. Y. (1970) Ultrastructural changes in cells of pea embryo radicles during germination. Journal of Cell Biology 45, 168–171.Google ScholarPubMed
Yu, W.J. and Greenwood, J.S. (1994) Purification and characterization of a cysteine proteinase involved in globulin hydrolysation in germinated Vicia faba L. Journal of Experimental Botany 45, 261–268.CrossRefGoogle Scholar