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Changes in the element composition of globoids and whole embryos in developing seeds of Cucurbita maxima

Published online by Cambridge University Press:  22 February 2007

Irene Ockenden ,
Marcia West ,
Jon Domingues  and
John N.A. Lott
Irene Ockenden
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
Marcia West
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
Jon Domingues
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
John N.A. Lott*
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
*
*Correspondence Tel: (905) 525-9140 ext. 24589 Fax: (905) 522-6066 Email: [email protected]
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Abstract

Cucurbita maxima Duch. cv. Warted Hubbard, which produces large fruits with many seeds, was used as a test system to study changes in several mineral nutrients during seed formation. Developing embryos increased markedly in weight, size and oil content. Energy dispersive X-ray (EDX) analysis was carried out on mineral nutrient storage globoids in developing Cucurbita cotyledons from 4 to 9 weeks after pollination. Phosphorus peak-to-background (p/b) and potassium p/b ratios increased slightly after the fourth week, while magnesium p/b ratios remained unchanged. The calcium p/b ratio was statistically higher at 4 weeks than in the subsequent weeks. Traces of iron and zinc were found in most of the globoids at all fruit ages. The zinc p/b ratio was higher in globoids at 4 weeks than at 9 weeks. Traces of manganese were detectable in only 18% of the globoids at 4 weeks and not detectable in any globoids at 9 weeks. The frequency of the small globoids of 0.25 μm in diameter was the highest at 4 weeks and was zero at 9 weeks. The frequency of the larger globoids increased progressively from 4 to 9 weeks. As the spherical globoids increased in size and hence in volume, there was a 6.4-fold increase in the embryo phytic acid, which is the main component of globoids. On a whole embryo basis, P content increased 4.5-fold, K 3.3-fold, Mg 5.4-fold and Ca 3.2-fold from the fourth to the ninth week.

Keywords

Cucurbita maximadeveloping seedsgloboidsenergy dispersive X-ray microanalysiselement composition
Type
Research Article
Information
Seed Science Research , Volume 11 , Issue 1 , March 2001 , pp. 35 - 44
Copyright
Copyright © Cambridge University Press 2001

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References

Association of Official Analytical Chemists (1990) AOAC official methods of analysis (15th edition), pp. 56, 800–801. Arlington, VA, AOAC International.Google Scholar
Bair, C.W. and Snyder, H.E. (1980) Electron microscopy of soybean lipid bodies. Journal of the American Oil Chemists Society 57, 279–282.CrossRefGoogle Scholar
Beecroft, P. and Lott, J.N.A. (1996) Changes in the element composition of globoids from Cucurbita maxima and Cucurbita andreana cotyledons during early seedling growth. Canadian Journal of Botany 74, 838–847.CrossRefGoogle Scholar
Bush, D.S. (1995) Calcium regulation in plant cells and its role in signaling. Annual Review of Plant Physiology and Plant Molecular Biology 46, 95–122.CrossRefGoogle Scholar
Greenwood, J.S. (1983) Phytin deposition within the protein bodies of the developing endosperm of castor bean (Ricinus communis cv. Hale). PhD Thesis, University of Calgary.Google Scholar
Greenwood, J.S. and Bewley, J.D. (1984) Subcellular distribution of phytin in the endosperm of developing castor bean: a possibility for its synthesis in the cytoplasm prior to deposition within protein bodies. Planta 160, 113–120.CrossRefGoogle ScholarPubMed
Greenwood, J.S., Gifford, D.J. and Bewley, J.D. (1984) Seed development in Ricinus communis cv. Hale (castor bean). II. Accumulation of phytic acid in the developing endosperm and embryo in relation to the deposition of lipid, protein, and phosphorus. Canadian Journal of Botany 62, 255–261.CrossRefGoogle Scholar
IUPAC and IUPAC-IUB (1968) The nomenclature of cyclitols. European Journal of Biochemistry 5, 1–12.CrossRefGoogle Scholar
Jacks, T.J. (1990) Cucurbit seeds: Cytological, physiochemical, and nutritional characterizations. pp. 356–363in Bates, D.M.Robinson, R.W.Jeffrey, C. (Eds) Biology and utilization of the Cucurbitaceae. Ithaca, Cornell University Press.Google Scholar
Lazlo, J.A. (1990) Mineral contents of soybean seed coats and embryos during development. Journal of Plant Nutrition 13, 231–248.CrossRefGoogle Scholar
Lott, J.N.A., Greenwood, J.S., Vollmer, C.M. and Buttrose, M.S. (1978) Energy-dispersive x-ray analysis of phosphorus, potassium, magnesium, and calcium in globoid crystals in protein bodies from different regions of Cucurbita maxima embryos. Plant Physiology 61, 984–988.CrossRefGoogle ScholarPubMed
Lott, J.N.A., Spitzer, E. and Vollmer, C.M. (1979) Calcium distribution in globoid crystals of Cucurbita cotyledon protein bodies. Plant Physiology 63, 847–851.CrossRefGoogle ScholarPubMed
Lott, J.N.A., Goodchild, D.J. and Craig, S. (1984) Studies of mineral reserves in pea (Pisum sativum) cotyledons using low-water-content procedures. Australian Journal of Plant Physiology 11, 459–469.Google Scholar
Lott, J.N.A., Randall, P.J., Goodchild, D.J. and Craig, S. (1985) Occurrence of globoid crystals in cotyledonary protein bodies of Pisum sativum as influenced by experimentally induced changes in Mg, Ca and K contents of seeds. Australian Journal of Plant Physiology 12, 341–353.Google Scholar
Lott, J.N.A., Ockenden, I., Kerr, P., West, M., Leech, T. and Skilnyk, H. (1994) The influence of experimentally induced changes in the (Mg + Ca):K balance on protein bodies formed in developing Cucurbita seeds. Canadian Journal of Botany 72, 364–369.CrossRefGoogle Scholar
Lott, J.N.A., Greenwood, J.S. and Batten, G.D. (1995) Mechanisms and regulation of mineral nutrient storage during seed development. pp. 215–235in Kigel, J.Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Lott, J.N.A., Ockenden, I., Raboy, V. and Batten, G.D. (2000) Phytic acid and phosphorus in crop seeds and fruits: a global estimate. Seed Science Research 10, 11–33.CrossRefGoogle Scholar
Makower, R.U. (1969) Changes in phytic acid and acidsoluble phosphorus in maturing pinto beans. Journal of the Science of Food and Agriculture 20, 82–84.CrossRefGoogle ScholarPubMed
Marschner, H. (1995) Mineral nutrition of higher plants. London, Academic Press.Google Scholar
Martin, C.J. and Evans, W.J. (1986) Phytic acid-metal ion interactions. II. The effect of pH on Ca(II) binding. Journal of Inorganic Biochemistry 27, 17–30.CrossRefGoogle ScholarPubMed
Nield, H. and Lott, J.N.A. (1989) Distribution of minerals within different regions of Cucurbita maxima fruits. Communications in Soil Science and Plant Analysis 20, 1085–1100.CrossRefGoogle Scholar
Ockenden, I. and Lott, J.N.A. (1988 a) Changes in the distribution of magnesium, potassium, calcium and phosphorus during growth of Cucurbita seedlings. Journal of Experimental Botany 39, 973–980.CrossRefGoogle Scholar
Ockenden, I. and Lott, J.N.A. (1988 b) Mineral storage in Cucurbita embryos. III. Calcium storage as compared with storage of magnesium, potassium, and phosphorus. Canadian Journal of Botany 66, 1486–1489.CrossRefGoogle Scholar
Ockenden, I. and Lott, J.N.A. (1990) Elemental storage in Cucurbita embryos: X-ray microanalysis of magnesium, potassium, calcium, and phosphorus within globoid crystals. Canadian Journal of Botany 68, 646–650.CrossRefGoogle Scholar
Ockenden, I., Falk, D.E. and Lott, J.N.A. (1997) Stability of phytate in barley and beans during storage. Journal of Agricultural and Food Chemistry 45, 1673–1677.CrossRefGoogle Scholar
Ogawa, M., Tanaka, K. and Kasai, Z. (1979 a) Energydispersive x-ray analysis of phytin globoids in aleurone particles of developing rice grains. Soil Science and Plant Nutrition 25, 437–448.CrossRefGoogle Scholar
Ogawa, M., Tanaka, K. and Kasai, Z. (1979 b) Accumulation of phosphorus, magnesium and potassium in developing rice grains: Followed by electron microprobe x-ray analysis focusing on the aleurone layer. Plant and Cell Physiology 20, 19–27.Google Scholar
Raboy, V. and Dickinson, D.B. (1984) Effect of phosphorus and zinc nutrition on soybean seed phytic acid and zinc. Plant Physiology 75, 1094–1098.CrossRefGoogle ScholarPubMed
Reid, D.A., Lott, J.N.A., Attree, S.M. and Fowke, L.C. (1999) Mineral nutrition in white spruce (Picea glauca [Moench] Voss) seeds and somatic embryos. I. Phosphorus, phytic acid, potassium, magnesium, calcium, iron and zinc. Plant Science 141, 11–18.CrossRefGoogle Scholar
Roberts, E.H. and Roberts, D.L. (1972) Moisture content of seeds. pp. 424–429in Roberts, E. H. (Ed.) Viability of seeds. Syracuse, NY, Syracuse University Press.Google Scholar
Saio, K. (1964) The change in inositol phosphates during the ripening of rice grains. Plant and Cell Physiology 5, 393–400.Google Scholar
Sale, P.W.G. and Campbell, L.C. (1980) Patterns of mineralnutrient accumulation in soybean seed. Field Crops Research 3, 157–163.CrossRefGoogle Scholar
Singh, B. (1953) Studies on the structure and development of seeds of Cucurbitaceae. Phytomorphology 3, 224–239.Google Scholar
Skilnyk, H.R. and Lott, J.N.A. (1992) Mineral analyses of storage reserves of Cucurbita maxima and Cucurbita andreana pollen. Canadian Journal of Botany 70, 491–495.CrossRefGoogle Scholar
Splittstoesser, W.E. (1982) The appearance of phytase and the changes in phytate and inorganic phosphorus during germination and early seedling growth of pumpkin (Cucurbita moschata Poir.). HortScience 17, 402–403.CrossRefGoogle Scholar
Stewart, A., Nield, H. and Lott, J.N.A. (1988) An investigation of the mineral content of barley grains and seedlings. Plant Physiology 86, 93–97.CrossRefGoogle ScholarPubMed
Wada, T. and Lott, J.N.A. (1997) Light and electron microscopic and energy dispersive x-ray microanalysis studies of globoids in protein bodies of embryo tissues and the aleurone layer of rice (Oryza sativa L.) grains. Canadian Journal of Botany 75, 1137–1147.CrossRefGoogle Scholar
Wada, T. and Maeda, E. (1987) Cyto-histological studies on phytin-containing particles in developing rice grains. Japanese Journal of Crop Science 56, 503–511.CrossRefGoogle Scholar
Zar, J.H. (1984) Biostatistical analysis (2nd edition) Englewood Cliffs, NJ, Prentice-Hall.Google Scholar