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Changes in activities of antioxidant enzymes and lipoxygenase during growth of sunflower seedlings from seeds of different vigour

Published online by Cambridge University Press:  22 February 2007

Christophe Bailly ,
Renata Bogatek-Leszczynska ,
Daniel Côme  and
Françoise Corbineau
Christophe Bailly*
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, tour 53, 1orétage, 4 place Jussieu, 75252 Paris cédex 05,France
Renata Bogatek-Leszczynska
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, tour 53, 1orétage, 4 place Jussieu, 75252 Paris cédex 05,France
Daniel Côme
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, tour 53, 1orétage, 4 place Jussieu, 75252 Paris cédex 05,France
Françoise Corbineau
Affiliation:
Physiologie Végétale Appliquée, Université Pierre et Marie Curie, tour 53, 1orétage, 4 place Jussieu, 75252 Paris cédex 05,France
*
*Correspondence Fax: + 33 1 44 27 59 27 Email: [email protected]
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Abstract

The aim of this study was to investigate whether there was a relationship between growth of sunflower seedlings at 15°C in the dark and activities of enzymes involved in scavenging of reactive oxygen species (ROS), especially superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR), or in production of free radicals, namely lipoxygenase (LOX). Untreated control seeds were compared with seeds exposed to accelerated ageing (5 d at 45°C and 100% relative humidity), osmopriming (7 d at 15°C with a polyethylene glycol (PEG) solution at –2 MPa) and accelerated ageing followed by priming. Accelerated ageing decreased seed germinability and slowed down hypocotyl growth, whereas priming resulted in an increase in germination rate and enhanced seedling development. Osmopriming of aged seeds almost completely restored the initial rate of germination and seedling growth. The activity of all the enzymes studied increased during seed germination and seedling development, except that of SOD. Seed imbibition or radicle protrusion were related mainly with an increase in CAT activity and, to a lesser extent, in GR activity. Increase of LOX activity was clearly associated with the onset of hypocotyl elongation. However, in all cases, malondialdehyde measurements did not reveal intense lipid peroxidation. Priming induced a marked stimulation of CAT and GR during seed imbibition or very early during seedling development, as compared to the control seedlings and particularly to the seedlings generated by aged seeds. Hydrogen peroxide (H2O2) contents of seeds and seedlings were closely correlated to the activities of CAT and GR and to the kinetics of seedling development. The results obtained establish a clear relationship between sunflower seed vigour and ROS scavenging.

Keywords

catalaseglutathione reductaseHelianthus annuuslipoxygenaseseedling growthseed vigoursuperoxide dismutase
Type
Research Article
Information
Seed Science Research , Volume 12 , Issue 1 , March 2002 , pp. 47 - 55
Copyright
Copyright © Cambridge University Press 2002

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References

Bailly, C., Benamar, A., Corbineau, F. and Côme, D. (1996) Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiologia Plantarum 97, 104–110.CrossRefGoogle Scholar
Bailly, C., Benamar, A., Corbineau, F. and Côme, D. (1998) Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds. Physiologia Plantarum 104, 646–652.CrossRefGoogle Scholar
Bailly, C., Benamar, A., Corbineau, F. and Côme, D. (2000) Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Science Research 10, 35–42.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1994). Seeds. Physiology of development and germination (2nd edition). New York, Plenum Press.CrossRefGoogle Scholar
Bradford, K.J. (1986) Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience 21, 1105–1112.CrossRefGoogle Scholar
Chateigner, A.L., Le Deunff, Y. and Jalouzot, R. (1999) Germination-associated changes in transcript content of pea seedling lipoxygenases. Lipoxygenase-g: a new marker of axis growth resumption. Planta 208, 606–613.CrossRefGoogle Scholar
Chojnowski, M., Corbineau, F. and Côme, D. (1997) Physiological and biochemical changes induced in sunflower seeds by osmopriming and subsequent drying, storage and aging. Seed Science Research 7, 323–331.CrossRefGoogle Scholar
Côme, D. and Thévenot, C. (1982). (and Environmental control of embryo dormancy and germination. pp. 271–298in Khan, A.A. (Ed.) The physiology and biochemistry of seed development, dormancy and germination. Amsterdam, Elsevier/North Holland.Google Scholar
Corbineau, F., Bagniol, S. and Côme, D. (1990) Sunflower (Helianthus annuus L.) seed dormancy and its regulation by ethylene. Israel Journal of Botany 39, 313–325.Google Scholar
Desikan, R., Mackerness, S.A.H., Hancock, J.T. and Neill, S.J. (2001) Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiology 127, 159–172.CrossRefGoogle ScholarPubMed
Eising, R., Trelease, R.N. and Ni, W. (1990) Biogenesis of catalase in glyoxysomes and leaf-type peroxisomes of sunflower cotyledons. Archives of Biochemistry and Biophysics 278, 258–264.CrossRefGoogle ScholarPubMed
Feussner, I. and Kindl, H. (1992) A lipoxygenase is the main lipid body protein in cucumber and soybean cotyledons during the stage of triglyceride mobilization. FEBS Letters 298, 223–225.CrossRefGoogle ScholarPubMed
Feussner, I., Balkenhohl, T.J., Porzel, A. and Wasternack, C. (1997) Structural elucidation of oxygenated storage lipids in cucumber cotyledons. Implication of lipid body lipoxygenase in lipid mobilization during germination. Journal of Biological Chemistry 272, 21635–21641.CrossRefGoogle ScholarPubMed
Fujikura, Y. and Karssen, C.M. (1992) Effects of controlled deterioration and osmopriming on protein synthesis of cauliflower during early germination. Seed Science Research 2, 23–31.CrossRefGoogle Scholar
Gallego, S.M., Benavides, M.P. and Tomaro, M.L. (1996) Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress. Plant Science 121, 151–159.CrossRefGoogle Scholar
Heath, R.L. and Parker, L. (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stochiomestry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125, 189–198.CrossRefGoogle ScholarPubMed
Hildebrand, D.F. (1989) Lipoxygenases. Physiologia Plantarum 76, 249–253.CrossRefGoogle Scholar
Inzé, D. and Van Montagu, M. (1995) Oxidative stress in plants. Current Opinion in Biotechnology 6, 153–158.CrossRefGoogle Scholar
Matsui, K., Irie, M., Kajiwara, T. and Hatanaka, A. (1992) Developmental changes in lipoxygenase activity in cotyledons of cucumber seedlings. Plant Science 85, 23–32.CrossRefGoogle Scholar
Matsui, K., Hijiya, K., Tabuchi, Y. and Kajiwara, T. (1999) Cucumber cotyledon lipoxygenase during postgerminative growth. Its expression and action on lipid bodies. Plant Physiology 119, 1279–1287.CrossRefGoogle ScholarPubMed
Melan, M.A., Enriquez, A.L.D. and Peterman, T.K. (1994) The LOX1 gene of Arabidopsis is temporally and spatially regulated in germinated seedlings. Plant Physiology 105, 385–393.CrossRefGoogle Scholar
O'Kane, D., Gill, V., Boyd, P. and Burdon, R. (1996) Chilling, oxidative stress and antioxidant responses in Arabidopsis thaliana callus. Planta 198, 371–377.CrossRefGoogle ScholarPubMed
Olsen, L.J. and Harada, J.J. (1995) Peroxisomes and their assembly in higher plants. Annual Review of Plant Physiology and Plant Molecular Biology 46, 123–146.CrossRefGoogle Scholar
Parera, C.A. and Cantliffe, D.J. (1994) Presowing seed priming. Horticultural Review 16, 109–141.Google Scholar
Rodriguez-Rosales, M.P., Kerkeb, L., Ferrol, N. and Donaire, J.P. (1998) Lipoxygenase activity and lipid composition of cotyledons and oil bodies of two sunflower hybrids. Plant Physiology and Biochemistry 36, 285–291.CrossRefGoogle Scholar
Rosahl, S. (1996) Lipoxygenases in plants – their role in development and stress response. Zeitschrift für Naturforschung 51, 123–138.CrossRefGoogle ScholarPubMed
Scandalios, J.G. (1997). Oxidative stress and the molecular biology of antioxidant defenses. New York, Cold Spring Harbor Laboratory Press.Google Scholar
Scandalios, J.G., Guan, L. and Polidoros, A.N. (1997). Catalases in plants: gene structure, properties, regulation and expression. pp. 343–406in Scandalios, J.G. (Ed.) Oxidative stress and the molecular biology of antioxidant defenses. New York, Cold Spring Harbor Laboratory Press.Google Scholar
Siedow, J.N. (1991) Plant lipoxygenase: structure and function. Annual Review of Plant Physiology and Plant Molecular Biology 42, 145–188.CrossRefGoogle Scholar
Smok, M.A., Chojnowski, M., Corbineau, F. and Côme, D. (1993). (, and Effects of osmotic treatment on sunflower seed germination in relation with temperature and oxygen. pp. 1033–1038in Côme, D.;, Corbineau, F. (Eds) Fourth international workshop on seeds. Basic and applied aspects of seed biology. Paris, ASFIS.Google Scholar
Thompson, J.E., Legge, R.L. and Barber, R.F. (1987) The role of free radicals in senescence and wounding. New Phytologist 105, 317–344.CrossRefGoogle ScholarPubMed
Tranbarger, T.J., Franceschi, V.R., Hildebrand, D.F. and Grimes, H.D. (1991) The soybean 94-kilodalton vegetative storage protein is a lipoxygenase that is localized in paraveinal mesophyll cell vacuoles. Plant Cell 3, 973–987.Google ScholarPubMed
Van Pijlen, J.G., Kraak, H.L., Bino, R.J. and De Vos, C.H.R. (1995) Effects of ageing and osmopriming on germination characteristics and chromosome aberrations of tomato (Lycopersicon esculentum Mill.) seeds. Seed Science and Technology 23, 823–830.Google Scholar