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Active oxygen and their scavengers in rice seeds (Oryza sativa cv. IET 4094) aged under tropical environmental conditions

Published online by Cambridge University Press:  19 September 2008

S. Nandi ,
S. Sen-Mandi  and
T.P. Sinha
S. Nandi
Affiliation:
Seed Biology Laboratory, Department of Botany
S. Sen-Mandi*
Affiliation:
Seed Biology Laboratory, Department of Botany
T.P. Sinha
Affiliation:
Department of Physics, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Calcutta – 700009, India
*
*Correspondence
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Abstract

Electron paramagnetic resonance studies of high vigour (99% viable) and low vigour (38% viable) dry embryos of rice seeds (Oryza sativa L.) stored in a natural (warm and humid) environment were carried out. Loss in viability due to hot and humid conditions was found to be correlated with a decrease in free radical levels. The free radicals could be carbon-based and derived from quinones. Presence of the active oxygen scavenging enzyme, superoxide dismutase (EC 1.15.1.1) in embryos isolated from dry seeds showed a positive corrrelation with the state of vigour or viability. Anodic peroxidase (EC 1.11.1.7) activity in imbibed seeds also declined with the decline in vigour and viability. It is concluded that the deterioration of cells in the embryonic axis depends on the balance between free radical accumulation and the activity of active oxygen-scavenging enzymes which constitutes the active oxygen scavenging system (AOSS) during early imbibition. During prolonged storage under hot and humid conditions, cumulative effects of macromolecular damage due to oxidative chain products, compounded with the loss of enzyme activity, result in the final catastrophe, the death of the embryo.

Keywords

Agingriceembryosfree radicalsactive oxygensuperoxide dismutaseperoxidase
Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 7 , Issue 3 , September 1997 , pp. 253 - 260
Copyright
Copyright © Cambridge University Press 1997

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