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A role for PM19-Like 1 in seed dormancy in Arabidopsis

Published online by Cambridge University Press:  26 July 2019

Jose M. Barrero Open the ORCID record for Jose M. Barrero [Opens in a new window] ,
Marie M. Dorr ,
Mark J. Talbot ,
Shinnosuke Ishikawa ,
Taishi Umezawa ,
Rosemary G. White  and
Frank Gubler
Jose M. Barrero*
Affiliation:
CSIRO Agriculture and Food, Canberra, ACT 2601, Australia
Marie M. Dorr
Affiliation:
CSIRO Agriculture and Food, Canberra, ACT 2601, Australia
Mark J. Talbot
Affiliation:
CSIRO Agriculture and Food, Canberra, ACT 2601, Australia
Shinnosuke Ishikawa
Affiliation:
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
Taishi Umezawa
Affiliation:
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
Rosemary G. White
Affiliation:
CSIRO Agriculture and Food, Canberra, ACT 2601, Australia
Frank Gubler
Affiliation:
CSIRO Agriculture and Food, Canberra, ACT 2601, Australia
*
*Author for correspondence: Jose Barrero, Email: [email protected]
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Abstract

The understanding of the genetic basis of grain dormancy in wheat has rapidly improved in the last few years, and a number of genes have been identified related to that trait. We recently identified the wheat genes TaPM19-A1 and -A2 and we have now taken the first step towards understanding the role of this class of genes in seeds. By investigating the Arabidopsis homologous PM19-Like 1 (PM19L1) we have found that it has a seed-specific expression pattern and, while its expression is higher in dormant than in non-dormant seeds, knock-out mutations produced seeds with increased dormancy. Not only primary dormancy, but also secondary dormancy in response to high temperature was increased by the loss-of-function. We have also examined the function of PM19L1 by localizing the PM19 protein primarily to the cotyledon cells in seeds, possibly in membranes. By investigating the co-expression network of this gene we have found that it is connected to a small group of abscisic acid (ABA)-induced seed maturation and storage-related genes. The function of PM19L1 represents a good opportunity to explore the interactions of key factors that can influence seed dormancy such as ABA, temperature and membrane properties.

Keywords

ABAAWPM19dormancygerminationmembranetemperature
Type
Research Paper
Information
Seed Science Research , Volume 29 , Issue 3 , September 2019 , pp. 184 - 196
Copyright
Copyright © Cambridge University Press 2019 

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Footnotes

‡

Current address: Universite Laval, Quebec, Canada.

§

Current address: Sunrice, Leeton, NSW, Australia.

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