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Removal of the testa during germination or establishment increases germinant mortality, decay and water loss

Published online by Cambridge University Press:  19 September 2008

B. B. Lamont  and
P. Milberg
B. B. Lamont*
Affiliation:
School of Environmental Biology, Curtin University of Technology, GPO Box U1987, Perth 6001, Australia
P. Milberg
Affiliation:
School of Environmental Biology, Curtin University of Technology, GPO Box U1987, Perth 6001, Australia Department of Crop Production Science, Swedish University of Agricultural Sciences (SLU), Box 7043, 750 07 Uppsala, Sweden
*
*Correspondence
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Abstract

By means of direct manipulation, this study showed that the testa protects the germinating seed and germinant from decay (an advantage in moist soils) and desiccation (an advantage in periodically dry soils) in four species, all of which retain their testas until the cotyledons are lifted above the soil. Furthermore, the testa prevents early spreading of the cotyledons (an advantage when elongating through soil and litter). Almost all germinants of Banksia hookeriana and Leucadendron eucalyptifolium, with their testas removed, died and rotted away after being sown on the soil surface or at a range of depths. Hakea cyclocarpa and H. strumosa showed only minor losses compared with the intact germinants. All naked germinants were smaller than the controls at any depth, except among H. cyclocarpa. Absence of the testa did not differentially affect the length of the hypocotyl in the dark or light, but lateral spread of the cotyledons was accelerated, especially in the dark. Removal of a small fraction of the testa was sufficient to cause general mortality of B. hookeriana germinants but not of H. cyclocarpa or H. strumosa. After imbibition, water content of the naked embryos of B. hookeriana reverted to the air-dry state within 24 h of sowing in air-dry sand, while intact seeds were still moist even after 7 days of drying. Compared with intact seeds, drying periods of 3–7 days decreased the amount of germination and root growth of naked embryos after rewetting, and increased microbial infection of the survivors. Removal of the testa from germinants was sufficient to cause general mortality after only 6 h in air-dry sand. In contrast, most intact germinants with short radicles recovered after drying for 2 days.

Keywords

Banksia hookerianadesiccationHakea cyclocarpaHakea strumosaLeucadendron eucalyptifoliumseed coatseedling
Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 7 , Issue 3 , September 1997 , pp. 245 - 252
Copyright
Copyright © Cambridge University Press 1997

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References

Crevecoeur, M., Deltour, R. and Bronchart, R. (1976) Cytological study of water stress during germination of Zea mays, Planta 132, 289–296.CrossRefGoogle ScholarPubMed
Dalling, J.W., Swaine, M.D. and Garwood, N.C. (1994) Effect of soil depth on seedling emergence in tropical soil seed-bank investigations. Functional Ecology 9, 119–121.CrossRefGoogle Scholar
George, A.S. (1981) The genus Banksia L.f. Nuytsia 3, 239–473.CrossRefGoogle Scholar
Halloin, J.M. (1986) Microorganisms and seed deterioration. pp 89–99in McDonald, M.B. and Nelson, C.J. (Eds) Physiology of seed deterioration. Wisconsin, Soil Science Society of America.Google Scholar
Johnson, L.A.S. and Briggs, B.G. (1975) On the Proteaceae — the evolution and classification of a southern family. Botanical Journal of the Linnean Society 70, 83–182.CrossRefGoogle Scholar
Kraft, J.M. (1977) The role of delphinidin and sugars in the resistance of pea seedlings to Fusarium root rot. Phytomorphology 67, 1057–1061.Google Scholar
Lamont, B.B., Witkowski, E.T.F. and Enright, N.J. (1993) Post-fire litter microsites: safe for seeds, unsafe for seedlings. Ecology 74, 501–512.CrossRefGoogle Scholar
Leprince, O., Hendry, G.A. and McKersie, B.D. (1993) The mechanisms of desiccation tolerance in developing seeds. Seed Science Research 3, 231–246.CrossRefGoogle Scholar
Lubbock, J. (1892) On seedlings, a contribution to our knowledge of seedlings. Vol. 2. Kegan Paul, Trench, Trubner.CrossRefGoogle Scholar
Mohamed-Yassen, Y., Barringer, S.A., Splittstoesser, W.E. and Costanza, S. (1994) The role of seed coats in seed viability. Botanical Review 60, 426–439.CrossRefGoogle Scholar
Richards, M.B. and Lamont, B.B. (1996) Post-fire mortality and water relations of three congeneric shrub species under extreme water stress — a tradeoff with fecundity. Oecologia 107, 53–60.CrossRefGoogle ScholarPubMed
Quartacci, M.F. and Navari-Izzo, F. (1992) Water stress and free radicle mediated changes in sunflower seedlings. Journal of Plant Physiology 139, 621–625.CrossRefGoogle Scholar