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Protection of Italian ryegrass (Lolium multiflorum L.) seedlings from salinity stress following seed priming with L-methionine and casein hydrolysate

Published online by Cambridge University Press:  07 December 2020

Keum-Ah Lee ,
Youngnam Kim ,
Hossein Alizadeh  and
David W.M. Leung Open the ORCID record for David W.M. Leung [Opens in a new window]
Keum-Ah Lee
Affiliation:
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch8040, New Zealand
Youngnam Kim
Affiliation:
Department of Ecology, Lincoln University, Lincoln7647, Christchurch, New Zealand
Hossein Alizadeh
Affiliation:
Bioprotection Research Centre, Lincoln University, PO BOX 85084, Canterbury, New Zealand
David W.M. Leung*
Affiliation:
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch8040, New Zealand
*
Correspondence: David W.M. Leung, E-mail: [email protected]
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Abstract

Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.

Keywords

abiotic stressantioxidative defencegerminationperoxidase activitysalt stress
Type
Research Paper
Information
Seed Science Research , Volume 31 , Issue 1 , March 2021 , pp. 51 - 59
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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References

Amjad, M, Ziaf, K, Iqbal, Q, Ahmad, I and Riaz, MA (2007) Effect of seed priming on seed vigour and salt tolerance in hot pepper. Pakistan Journal of Agricultural Sciences 44, 408–416.Google Scholar
Bose, J, Rodrigo-Moreno, A and Shabala, S (2014) ROS homeostasis in halophytes in the context of salinity stress tolerance. Journal of Experimental Botany 65, 1241–1257.CrossRefGoogle ScholarPubMed
Bradford, M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle Scholar
Chen, K and Arora, R (2013) Priming memory invokes seed stress-tolerance. Environmental and Experimental Botany 94, 33–45.CrossRefGoogle Scholar
Cheng, Y, Tiana, Q and Zhang, WH (2016) Glutamate receptors are involved in mitigating effects of amino acids on seed germination of Arabidopsis thaliana under salt stress. Environmental and Experimental Botany 130, 68–78.CrossRefGoogle Scholar
Daniel, MA, David, RHA, Caesar, SA, Ramakrishnan, M, Duraipandiyan, V, Ignacimuthu, S and Al-Dhabi, NA (2018) Effect of L-glutamine and casein hydrolysate in the development of somatic embryos from cotyledonary leaf explants in okra (Abelmoschus esculentus L. Monech). South African Journal of Botany 114, 223–231.CrossRefGoogle Scholar
Das, K and Roychoudhury, A (2014) Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science 2, 53.CrossRefGoogle Scholar
El-Awadi, ME and Hassan, EA (2010) Physiological responses of fennel (Foeniculum vulgara Mill) plants to some growth substances. The effect of certain amino acids and a pyrimidine derivative. American Journal of Science 6, 120–125.Google Scholar
Hayat, S, Hayat, Q, Alyemeni, MN, Wani, AS, Pichtel, J and Ahmad, A (2012) Role of proline under changing environments. A review. Plant Signal Behaviour 7, 1456–1466.CrossRefGoogle ScholarPubMed
Hozayn, M and Ahmed, AA (2019) Effect of magneto-priming by tryptophan and ascorbic acid on germination attributes of barley (Hordeum vulgare, L.) under salinity stress. EurAsian Journal of Biological Sciences 13, 245–251.Google Scholar
Hunt, WF and Easton, HS (1989) Fifty years of ryegrass research in New Zealand. Proceedings of the New Zealand Grasslands Association 50, 1–23.Google Scholar
Ibrahim, EA (2016) Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology 192, 38–46.CrossRefGoogle ScholarPubMed
Jafar, MZ, Farooq, M, Cheema, MA, Afzal, I, Basra, SMA, Wahid, MA, Aziz, T and Shahid, M (2012) Improving the performance of wheat by seed priming under saline conditions. Journal of Agronomy and Crop Science 198, 38–45.CrossRefGoogle Scholar
Jisha, KC, Vijayakumari, K and Puthur, JT (2013) Seed priming for abiotic stress tolerance: an overview. Acta Physiologia Plantarum 35, 1381–1396.CrossRefGoogle Scholar
Kovács, E, Nyitrai, P, Czövek, P, Óvári, M and Keresztes, Á (2009) Investigation into the mechanism of stimulation by low-concentration stressors in barley seedlings. Journal of Plant Physiology 166, 72–79.CrossRefGoogle ScholarPubMed
Li, B, Ishii, Y, Idota, S, Tobisa, M, Niimi, M, Yang, Y and Nishimura, K (2019) Yield and quality of forages in a triple cropping system in southern Kyushu Japan. Agronomy 9, 277. https://doi.org/10.3390/agronomy906027.CrossRefGoogle Scholar
Li, R, Min, D, Chen, L, Chen, C and Hu, X (2017) Hydropriming accelerates seed germination of Medicago sativa under stressful conditions: a thermal and hydrotime model approach. Legume Research 40, 741–747.Google Scholar
Nasibi, F, Kabantari, KM, Zanganeh, R, Mohammaad-Nejad, G and Oloumi, H (2012) Seed priming with cysteine modulates the growth and metabolic activity of wheat plants under salinity and osmotic stresses at early stages of growth. Indian J Plant Physiol 21, xx–xx.Google Scholar
Mahmoudi, H, Ben Salah, I, Zaouali, W, Hamrouni, L, Gruber, M, Ouerghi, Z and Hosni, K (2019) Priming-induced changes in germination, morpho-physiological and leaf biochemical responses of fenugreek (Trigonella foenum-graecum) under salt stress. Plant Biosystems – An International Journal Dealing with all Aspects of Plant Biology, doi:10.1080/11263504.2019.1651785.Google Scholar
Mittova, V, Guy, M, Tal, M and Volokita, M (2004) Salinity up-regulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii. Journal of Experimental Botany 55, 1105–1113.CrossRefGoogle ScholarPubMed
Moreno, C, Seal, CE and Papenbrock, J (2018) Seed priming improves germination in saline conditions for Chenopodium quinoa and Amaranthus caudatus. Journal of Agronomy and Crop Science 204, 40–48.CrossRefGoogle Scholar
Munns, R and Tester, M (2008) Mechanisms of salinity tolerance. Annual Review of Plant Biology 59, 651–681.CrossRefGoogle ScholarPubMed
Ogawa, S and Mitsuta, S (2012) S-methylmethionine is involved in the salinity tolerance of Arabidopsis thaliana plants at germination and early growth stages. Physiologia Plantarum 144, 13–19.CrossRefGoogle ScholarPubMed
Oliveira, CES, Steiner, F, Zuffao, AM, Zoz, T, Alves, CZ and De Aguiar, VCB (2019) Seed priming improves the germination and growth rate of melon seedlings under saline stress. Ciencia Rural 49, e20180588.CrossRefGoogle Scholar
Pratelli, R and Pilot, G (2014) Regulation of amino acid metabolic enzymes and transporters in plants. Journal of Experimental Botany 65, 5535–5556.CrossRefGoogle ScholarPubMed
Roy, D, Basu, N, Bhunia, A and Banerjee, S (1993) Counteraction of exogenous L-proline with NaCl in salt-sensitive cultivar of rice. Biologia Plantarum 35, 69–72.CrossRefGoogle Scholar
Shafiq, F, Raza, SH, Bibi, A, Khan, I and Iqbal, M (2018) Influence of proline priming on antioxidative potential and ionic distribution and its relationship with salt tolerance of wheat. Cereal Research Communications 46, 287–300.CrossRefGoogle Scholar
Shu, K, Qi, Y, Chen, F, Meng, Y, Luo, X, Shuai, H, Zhou, W, Ding, J, Du, J, Liu, J, Yang, F, Wang, Q, Liu, W, Yong, T, Wang, X, Feng, Y and Yang, W (2017) Salt stress represses soybean seed germination by negatively regulating GA biosynthesis while positively mediating ABA biosynthesis. Frontiers in Plant Science 8, 1372.CrossRefGoogle ScholarPubMed
Takahashi, W, Oishi, H, Ebina, M, Komatsu, T and Takamizo, T (2010) Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysia grass. Breed Science 60, 279–285.CrossRefGoogle Scholar
Tegeder, M (2012) Transporters for amino acids in plant cells: some functions and many unknowns. Current Opinion in Plant Biology 15, 315–321.CrossRefGoogle ScholarPubMed
Thom, ER and Prestidge, RA (1996) Use of Italian ryegrass on seasonal dairy farms in northern New Zealand. 1. Feed production and persistence. New Zealand Journal of Agricultural Research 39, 223–236.CrossRefGoogle Scholar
Van Breusegem, F and Dat, JF (2006) Reactive oxygen species in plant cell death. Plant Physiology 141, 384–390.CrossRefGoogle ScholarPubMed
Wang, H, Tang, X, Wang, H and Shao, HB (2015a) Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress. Frontiers in Plant Science 6, https://doi.org/10.3389/fpls.2015.00792.CrossRefGoogle Scholar
Wang, M, Wu, C, Cheng, Z and Meng, H (2015b) Growth and physiological changes in continuously cropped eggplant (Solanum melongena L.) upon relay intercropping with garlic (Allium sativum L.). Frontiers in Plant Science 6, 262.Google Scholar