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Identification of the sites of K leakage from imbibing seeds and grains

Published online by Cambridge University Press:  19 September 2008

Penny Beecroft  and
John N. A. Lott
Penny Beecroft*
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario, L8S 4K1, Canada
John N. A. Lott
Affiliation:
Department of Biology, McMaster University, Hamilton, Ontario, L8S 4K1, Canada
*
* Correspondence
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Abstract

Seeds/grains of four species were imbibed for up to 90 minutes half embedded in agar. The agar was then freeze-dried and treated with a chromatographic reagent to detect the sites of potassium leakage from the imbibing seeds/grains. Soybean (Glycine max cv. Marathon) and pea (Pisum sativum cv. Little Marvel) leaked K across the entire surface of their testas. Aged pea seeds leaked much more extensively than seeds of a fresher lot of the same cultivar. Squash (Cucurbita maxima cv. Warted Hubbard) seeds leaked extensively from the flat sides of the seeds and at the hilum, but only slightly at the margins. Maize (Zea mays cv. Golden Beauty) leaked most extensively across the endosperm-only side (i.e. the side opposite the embryo) of the kernel, and kernels leaked more at the tip-cap end than at the broad end. Energy dispersive X-ray analysis determined that K was present in the testas/pericarps before imbibition in all species studied, and that the peak-to-background ratios of K were lower after the seeds/grains had been exposed to water. Neutron activation analysis verified that K was leaked out of the seeds/grains and absorbed into the agar. Seeds from all species studied showed varying amounts of seed-to-seed variation. These variations can be attributed in part to differences in testa/pericarp structure and condition.

Keywords

grainsimbibitionleakage from seedspotassiumseedssites of leakage
Type
Research Papers
Information
Seed Science Research , Volume 3 , Issue 2 , June 1993 , pp. 105 - 110
Copyright
Copyright © Cambridge University Press 1993

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References

Bruggink, H., Kraak, H.L., Dijkema, H.M.G.E. and Bekendam, J. (1991) Some factors influencing electrolyte leakage from maize (Zea mays L.) kernels. Seed Science Research 1, 15–20.CrossRefGoogle Scholar
Calero, E., West, S.H. and Hinson, K. (1981) Water absorption of soybean seeds and associated causal factors. Crop Science 21, 926–933.CrossRefGoogle Scholar
Duke, S.H. and Kakefuda, G. (1981) Role of the testa in preventing cellular rupture during imbibition of legume seeds. Plant Physiology 67, 449–456.CrossRefGoogle ScholarPubMed
Duke, S.H., Kakefuda, G. and Harvey, T.M. (1983) Differential leakage of intracellular substances from imbibing soybean seeds. Plant Physiology 72, 919–924.CrossRefGoogle ScholarPubMed
Givelberg, A., Horowitz, M. and Poljakoff-Mayber, A. (1984) Solute leakage from Solanum nigrum L. seeds exposed to high temperatures during imbibition. Journal of Experimental Botany 35, 1754–1763.CrossRefGoogle Scholar
Hunter, J.R. and Erickson, A.E. (1952) Relation of seed germination to soil moisture tension. Agronomy Journal 44, 107–109.CrossRefGoogle Scholar
Larson, L.A. (1968) The effect soaking pea seeds with or without seed coats has on seedling growth. Plant Physiology 43, 253–259.CrossRefGoogle ScholarPubMed
Loomis, E.L. and Smith, O.E. (1980) The effect of artificial aging on the concentration of Ca, Mg, Mn, K and Cl in imbibing cabbage seed. Journal of the American Society for Horticultural Science 105, 647–650.CrossRefGoogle Scholar
Lott, J.N.A. (1973) A scanning electron microscope study of Cucurbita maxima seed coat structure. Canadian Journal of Botany 51, 1711–1714.CrossRefGoogle Scholar
Lott, J.N.A., Cavdek, V. and Carson, J. (1991) Leakage of K, Mg, Cl, Ca, and Mn from imbibing seeds, grains and isolated seed parts. Seed Science Research 1, 229–233.CrossRefGoogle Scholar
McKersie, B.D. and Stinson, R.H. (1980) Effect of dehydration on leakage and membrane structure in Lotus corniculatus L. seeds. Plant Physiology 66, 316–320.CrossRefGoogle ScholarPubMed
Miller, C.C. and Magee, R.J. (1951) Separations by partition chromatography on paper. Journal of the Chemical Society, 3183–3187.CrossRefGoogle Scholar
Mullett, J.H. and Considine, J.A. (1980) Potassium release and uptake in germinating legume seeds in relation to seed condition and germination environment. Journal of Experimental Botany 31, 151–162.CrossRefGoogle Scholar
Parrish, D.J. and Leopold, A.C. (1977) Transient changes during soybean imbibition. Plant Physiology 59, 1111–1115.CrossRefGoogle ScholarPubMed
Pearson, R. and Parkinson, D. (1961) The sites of excretion of ninhydrin-positive substances by broad bean seedlings. Plant and Soil 13, 391–396.CrossRefGoogle Scholar
Powell, A.A. and Matthews, S. (1981) A physical explanation for solute leakage from dry pea embryos during imbibition. Journal of Experimental Botany 32, 1045–1050.CrossRefGoogle Scholar
Powell, A.S., Oliveira, M.A. and Matthews, S. (1986) The role of imbibition damage in determining the vigour of white and coloured seed lots of dwarf french beans (Phaseolus vulgaris). Journal of Experimental Botany 37, 716–722.CrossRefGoogle Scholar
Schoettle, A.W. and Leopold, A.C. (1984) Solute leakage from artificially aged soybean seeds after imbibition. Crop Science 24, 835–839.CrossRefGoogle Scholar
Scroth, M.N. and Cook, R.J. (1964) Seed exudation and its influence on pre-emergence damping-off of bean. Phytopathology 54, 670–673.Google Scholar
Simon, E.W. and Mills, L.K. (1983) Imbibition, leakage and membranes. Recent Advances in Phytochemistry 17, 9–27.Google Scholar
Simon, E.W. and Raja Harun, R.M. (1972) Leakage during seed imbibition. Journal of Experimental Botany 23, 1076–1085.CrossRefGoogle Scholar
Spaeth, S.C. and Hughes, J.S. (1987) Cellular rupture and release of protoplasm and protein bodies from pea and bean cotyledons during imbibition. Food Microstructure 6, 127–134.Google Scholar
Stewart, A., Nield, H. and Lott, J.N.A. (1988) An investigation of the mineral content and barley grains and seedlings. Plant Physiology 86, 93–97.CrossRefGoogle ScholarPubMed
Swanson, B.G., Hughes, J.S. and Rasmussen, H.P. (1985) Seed microstructure: review of water imbibition in legumes. Food Microstructure 4, 115–124.Google Scholar
Yaklich, R.W., Vigil, E.L. and Wergin, W.P. (1985) Pore development and seed coat permeability in soybean. Crop Science 26, 616–624.CrossRefGoogle Scholar