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Expression and activity of a Kip-related protein, Zeama;KRP1, during maize germination

Published online by Cambridge University Press:  01 June 2008

Natividad de Jesús Juárez ,
Alfredo Mancilla ,
Elpidio García  and
Jorge M. Vázquez-Ramos
Natividad de Jesús Juárez
Affiliation:
Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, MéxicoD.F. 04510
Alfredo Mancilla
Affiliation:
Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, MéxicoD.F. 04510
Elpidio García
Affiliation:
Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, MéxicoD.F. 04510
Jorge M. Vázquez-Ramos*
Affiliation:
Facultad de Química, Departamento de Bioquímica, Universidad Nacional Autónoma de México, MéxicoD.F. 04510
*
*Correspondence Fax: +52 55 56225329[email protected]
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Abstract

Plant KRP proteins are cyclin/cyclin-dependent kinase subunit (Cdk) inhibitors that share a limited homology with mammalian p27Kip1 proteins. Several KRPs have been reported in maize (Zea mays L.), of which Zeama;KRP1 was studied during maize germination. Expression of the Zeama;KRP1 gene did not vary during the 24 h germination period. A homologous antibody raised against the 13 kDa carboxy end of the Zeama;KRP1 polypeptide, a sequence containing the cyclin/Cdk inhibitory domain, indicated the existence of a 22 kDa protein in maize embryonic axes, the amount of which also remained unchanged during germination. Neither abscisic acid nor cytokinins modified the amount of protein. The purified Zeama;KRP1 polypeptide inhibited the kinase activity associated with Zeama;PCNA and Zeama;CycD2;1, and also the kinase activity in p13Suc1-pulled-down complexes. However, there were differences in the inhibition pattern during germination. Whereas kinase activity in proliferating cell nuclear antigen (PCNA) or CycD2;1 immunoprecipitates was strongly inhibited mainly during early germination, that in p13Suc1-pulled-down complexes was mainly inhibited at late times, suggesting that each protein complex is composed of different cyclins and/or Cdks.

Keywords

cell cyclecyclin/CdkgerminationKRPsZea mays
Type
Research Article
Information
Seed Science Research , Volume 18 , Issue 2 , June 2008 , pp. 67 - 75
Copyright
Copyright © Cambridge University Press 2008

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