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Dormancy-break and germination in seeds of Prunus campanulata (Rosaceae): role of covering layers and changes in concentration of abscisic acid and gibberellins

Published online by Cambridge University Press:  01 March 2007

Shun-Ying Chen ,
Ching-Te Chien ,
Jeng-Der Chung ,
Yuh-Shyong Yang  and
Shing-Rong Kuo
Shun-Ying Chen
Affiliation:
Division of Forest Biology, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei, 10066, Taiwan School of Forestry and Resource Conservation, National Taiwan University, Taipei, 10617, Taiwan
Ching-Te Chien*
Affiliation:
Division of Silviculture, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei, 10066, Taiwan
Jeng-Der Chung
Affiliation:
Division of Silviculture, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei, 10066, Taiwan
Yuh-Shyong Yang
Affiliation:
Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 30068, Taiwan
Shing-Rong Kuo
Affiliation:
School of Forestry and Resource Conservation, National Taiwan University, Taipei, 10617, Taiwan
*
*Correspondence Fax: +886 2 23078742 Email: [email protected]
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Abstract

Intact seeds (seed+endocarp) from freshly harvested fruits of Prunus campanulata were dormant, and required 4–6 weeks of warm followed by 8 weeks of cold stratification for maximum germination percentage. Removing both endocarp and seed coat, however, promoted germination in a high percentage of non-stratified seeds. Treatment of intact, non-stratified seeds with gibberellic acid (GA3) was only partially effective in breaking dormancy. However, GA3 promoted germination of non-stratified seeds in which the endocarp (but not the seed coat) had been removed. The order of abscisic acid (ABA) concentration in fresh seeds was endocarp > seed coat > embryo, and its concentration in endocarp plus seed coat was about 6.2-fold higher than that in the embryo. Total ABA contents of seeds subjected to warm and/or cold moist stratification were reduced 6- to 12-fold. A higher concentration of GA4 was detected in embryos of non-dormant than in those of dormant seeds. Fluridone, a carotenoid biosynthesis inhibitor, was efficient in breaking dormancy of Prunus seeds. Paclobutrazol, a GA biosynthesis inhibitor, completely inhibited seed germination, and the inhibitory effect could be partially reversed by GA4, but not by GA3. Thus, dormancy in P. campanulata seeds is imposed by the covering layers. Dormancy break is accompanied by a decrease in ABA content of the covering layers and germination by an increase of embryonic GA4 content.

Keywords

abscisic acidcold and warm stratif icationendocarpfluridonegerminationgibberellinspaclobutrazolPrunus campanulataseed coatseed dormancy
Type
Research Article
Information
Seed Science Research , Volume 17 , Issue 1 , March 2007 , pp. 21 - 32
Copyright
Copyright © Cambridge University Press 2007

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