Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-02T23:58:09.876Z Has data issue: false hasContentIssue false
  • English
  • Français

The distribution of ATP within tomato (Lycopersicon esculentum Mill.) embryos correlates with germination whereas total ATP concentration does not

Published online by Cambridge University Press:  22 February 2007

Patrick Spoelstra ,
Ronny V.L. Joosen ,
Linus H.W. Van der Plas  and
Henk W.M. Hilhorst
Patrick Spoelstra
Affiliation:
Wageningen University, Laboratory of Plant Physiology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
Ronny V.L. Joosen
Affiliation:
Wageningen University, Laboratory of Plant Physiology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
Linus H.W. Van der Plas
Affiliation:
Wageningen University, Laboratory of Plant Physiology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
Henk W.M. Hilhorst*
Affiliation:
Wageningen University, Laboratory of Plant Physiology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
*
*Correspondence Fax: +31 317484740 Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The distribution of ATP in tomato seeds was visualized by monitoring the luminescence of frozen sections on top of a gel containing all the components of the luciferase reaction, but excluding ATP. ATP was imaged in germinating tomato seeds at intervals of 3, 6, 17, 24 and 48 h and in seeds with primary or secondary dormancy. ATP was present mainly in the embryo and concentrated in the radicle tip towards the completion of germination. In contrast to germinating seeds, ATP was distributed more evenly in dormant seeds. For germination, the ratio of ATP concentration in the radicle tip versus cotyledons was decisive, rather than the absolute concentration.

Keywords

Lycopersicon esculentumtomatoATPluciferasegerminationdormancy
Type
Research Article
Information
Seed Science Research , Volume 12 , Issue 4 , December 2002 , pp. 231 - 238
Copyright
Copyright © Cambridge University Press 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aflalo, C. (1991) Biological localized firefly luciferase: A tool to study cellular processes. International Review of Cytology 130, 269–323.CrossRefGoogle ScholarPubMed
Benjamin, L.R. (1990) Variation in time of seedling emergence within populations: a feature that determines individual growth and development. Advances in Agronomy 44, 1–25.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1994) Seeds. Physiology of development and germination (2nd edition). New York, Plenum Press.CrossRefGoogle Scholar
Ching, T.M. (1973) Adenosine triphosphate content and seed vigour. Plant Physiology 51, 400–402.CrossRefGoogle Scholar
Coolbear, P., Slater, R.J. and Bryant, J.A. (1990) Changes in nucleic acid levels associated with improved germination performance of tomato seeds after low temperature presowing treatment. Annals of Botany 65, 187–195.CrossRefGoogle Scholar
Dahal, P., Kim, N.S. and Bradford, K.J. (1996) Respiration and germination rates of tomato seeds at suboptimal temperatures and reduced water potentials. Journal of Experimental Botany 47, 941–947.CrossRefGoogle Scholar
De Castro, R.D. (1998) A functional analysis of cell cycle events in developing and germinating tomato seeds. Thesis, Wageningen University.Google Scholar
De Castro, R.D., Zheng, X., Bergervoet, J.H.W., De Vos, C.H.R. and Bino, R.J. (1995) β-tubulin accumulation and DNA replication in imbibing tomato seeds. Plant Physiology 109, 499–504.CrossRefGoogle ScholarPubMed
De Castro, R.D., Van Lammeren, A.A.M., Groot, S.P.C., Bino, R.J. and Hilhorst, H.W.M. (2000) Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not. Plant Physiology 122, 327–335.CrossRefGoogle ScholarPubMed
De Castro, R.D., Bino, R.J., Jing, H.C., Kieft, H. and Hilhorst, H.W.M. (2001) Depth of dormancy in tomato (Lycopersicon esculentum Mill.) seeds is related to the progression of the cell cycle prior to the induction of dormancy. Seed Science Research 11, 45–54.CrossRefGoogle Scholar
DeLuca, M. and McElroy, W.D.B (1974) Kinetics of the firefly luciferase catalyzed reactions. Biochemistry 13, 921–925.CrossRefGoogle ScholarPubMed
Derkx, M.P.M., Smidt, W.J., van der Plas, L.H.W. and Karssen, C.M. (1994) Changes in dormancy of Sisymbrium officinale seeds do not depend on changes in respiratory activity. Physiologia Plantarum 89, 707–718.CrossRefGoogle Scholar
Goldmark, P.J., Curry, J., Morris, C.F. and Walker-Simmons, M.K. (1992) Cloning and expression of an embryo-specific mRNA up-regulated in hydrated dormant seeds. Plant Molecular Biology 19, 433–441.CrossRefGoogle ScholarPubMed
Gould, S.J. and Subramani, S. (1988) Firefly luciferase as a tool in molecular and cell biology. Analytical Biochemistry 175, 5–13.CrossRefGoogle ScholarPubMed
Jain, J.C., Quick, W.A. and Hsaio, A.I. (1983) ATP synthesis during water imbibition in caryopses of genetically dormant and non-dormant lines of wild oat (Avena fatua L.). Journal of Experimental Botany 34, 381–387.CrossRefGoogle Scholar
Karssen, C.M. (1982) Seasonal patterns of dormancy in weed seeds. pp. 243–270in Khan, A.A. (Ed.) The physiology and biochemistry of seed development, dormancy and germination. Amsterdam, Elsevier Biomedical Press.Google Scholar
Li, B. and Foley, M.E. (1995) Cloning and characterization of differentially expressed genes in imbibed dormant and afterripened Avena fatua embryos. Plant Molecular Biology 29, 823–831.CrossRefGoogle ScholarPubMed
Liu, Y., Bergervoet, J.H.W., De Vos, C.H.R., Hilhorst, H.W.M., Kraak, H.L., Karssen, C.M. and Bino, R.J. (1994) Nuclear replication activities during imbibition of abscisic acid- and gibberellin-deficient tomato (Lycopersicon esculentum Mill.) seeds. Planta 194, 368–373.CrossRefGoogle Scholar
Lunn, G. and Madsen, E. (1981) ATP-levels of germinating seeds in relation to vigour. Physiologia Plantarum 53, 164–169.CrossRefGoogle Scholar
Mazor, L., Negbi, M. and Perl, M. (1984) The lack of correlation between ATP accumulation in seeds at the early stage of germination and seed quality. Journal of Experimental Botany 35, 1128–1135.CrossRefGoogle Scholar
Perl, M. (1986) ATP synthesis and utilization in the early stage of seed germination in relation to seed dormancy and quality. Physiologia Plantarum 66, 177–182.CrossRefGoogle Scholar
Saglio, P.H. and Pradet, A. (1980) Soluble sugars, respiration and energy charge during ageing of excised maize root tips. Plant Physiology 66, 516–519.CrossRefGoogle ScholarPubMed
Siegenthaler, P.A. and Douet-Orhant, V. (1994) Relationship between the ATP content measured at three imbibition times and germination of onion seeds during storage at 3, 15 and 30 degrees C. Journal of Experimental Botany 45, 1365–1371.CrossRefGoogle Scholar
Spoelstra, P., Joosen, R.V.L., Van der Krol, A.R. and Hilhorst, H.W.M. (1999) The firely luciferase-luciferin system as a tool to study the germination of single tomato seeds. p.112 in Proceedings of the VIth international workshop on seed biology, January 1999, Mérida, Mexico.Google Scholar
Still, D.W. and Bradford, K.J. (1997) Endo-beta-mannanase activity from individual tomato endosperm caps and radicle tips in relation to germination rates. Plant Physiology 113, 21–29.CrossRefGoogle ScholarPubMed
Still, D.W., Dahal, P. and Bradford, K.J. (1997) A single-seed assay for endo-beta-mannanase activity from tomato endosperm and radicle tissues. Plant Physiology 113, 13–20.CrossRefGoogle ScholarPubMed
Toorop, P.E., Bewley, J.D. and Hilhorst, H.W.M. (1996) Endo-beta-mannanase isoforms are present in the endosperm and embryo of tomato seeds, but are not essentially linked to the completion of germination. Planta 200, 153–158.CrossRefGoogle Scholar
Van der Krol, A.R. and Chua, N.H. (1991) The basic domain of plant B-ZIP proteins facilitates import of a reporter protein into plant nuclei. Plant Cell 3, 667–675.Google ScholarPubMed
Van Leeuwen, W., Hagendoorn, M.J.M., Ruttink, T., van Poecke, R., van der Plas, L.H.W. and van der Krol, A.R. (2000) The use of the firefly luciferase reporter system for in planta gene expression studies. Plant Molecular Biology Reporter 18, 1–20.CrossRefGoogle Scholar
Walenta, S., Doetsch, J. and Mueller-Klieser, W. (1990) ATP concentrations in multicellular tumor spheroids assessed by single photon imaging and quantitative bioluminescence. European Journal of Cell Biology 52, 389–393.Google ScholarPubMed