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Cytoplasmic glass formation in maize embryos

Published online by Cambridge University Press:  19 September 2008

F. Bruni  and
A. C. Leopold
F. Bruni*
Affiliation:
Section of Plant Biology, Cornell University, Ithaca, New York 14853, USA
A. C. Leopold
Affiliation:
Boyce-Thompson Institute, Cornell University, Ithaca, New York 14853, USA
*
* Correspondence and present address Dipartimento di Fisica, Universita di Roma ‘La Sapienza’, 00185 Rome, Italy
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Abstract

In order to examine the occurrence of a glassy state in the cytoplasm of maize embryos as a function of water content, isolated embryos were examined using spin-probe electron spin resonance. The glass transition temperature was determined at various degrees of hydration (h) in the range 0.05–0.25 g H2O g−1 dry sample weight. The obtained phase diagram indicates that, at standard storage temperature (−5°C), the cytoplasm of embryos drier than 0.15 g H2O g−1 is in a glassy state.

Type
Short Communication
Information
Seed Science Research , Volume 2 , Issue 4 , December 1992 , pp. 251 - 253
Copyright
Copyright © Cambridge University Press 1992

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References

Bruni, F. and Leopold, A.C. (1991a) Glass transitions in soybean seed. Relevance to anhydrous biology. Plant Physiology 96, 660–663.CrossRefGoogle ScholarPubMed
Bruni, F. and Leopold, A.C. (1991b) Hydration, protons and onset of physiological activities in maize seeds. Physiologia Plantarum 81, 359–366.CrossRefGoogle Scholar
Bruni, F. and Leopold, A.C. (1992) Pools of water in anhydrobiotic organisms. A thermally stimulated depolarization current study. Biophysical Journal 63, 663–672.CrossRefGoogle ScholarPubMed
Burke, M.J. (1986) The glassy state and survival of anhydrous biological systems. pp. 358–364 in Leopold, A.C. (Ed.) Membranes, metabolism, and dry organisms. Ithaca, Cornell University Press.Google Scholar
Ellis, R.H., Hong, T.D., Roberts, E.H. and Tao, K. (1990) Low moisture content limits to relations between seed longevity and moisture. Journal of Experimental Botany 65, 493–504.Google Scholar
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1991) Seed moisture content, storage, viability and vigour. Seed Science Research 1, 275–277.CrossRefGoogle Scholar
Franks, F. (1985) Biophysics and biochemistry at low temperatures. Cambridge, Cambridge University Press.Google Scholar
Freed, J.H. and Fraenkel, G.K. (1963) Theory of linewidths in electron spin resonance spectra. Journal of Chemical Physics 39, 326–348.CrossRefGoogle Scholar
Green, J.L. and Angell, C.A. (1989) Phase relations and vitrification in saccharide–water solutions and the trehalose anomaly. Journal of Physical Chemistry 93, 2880–2882.CrossRefGoogle Scholar
Koster, K.L. (1991) Glass formation and desiccation tolerance in seeds. Plant Physiology 96, 302–304.CrossRefGoogle ScholarPubMed
Leopold, A.C., Bruni, F. and Williams, R.J. (1992) Water in dry organisms. pp. 161–169 in Somero, G.N., Osmond, C.B. and Bolis, C.L. (Eds) Water and life. Berlin, Springer Verlag.CrossRefGoogle Scholar
MacKenzie, A.P. and Rasmussen, D.H. (1972) Interactions in the water–polyvinylpyrrolidone system at low temperature. pp. 147–171 in Jellineck, H.H.G. (Ed.) Water structure at the water–polymer interface. New York, Plenum Press.Google Scholar
Vertucci, C.W. (1989) Effects of cooling rate on seeds exposed to liquid nitrogen temperatures. Plant Physiology 90, 1478–1485.CrossRefGoogle ScholarPubMed
Vertucci, C.W. and Roos, E.E. (1990) Theoretical basis of protocols for seed storage. Plant Physiology 94, 1019–1023.CrossRefGoogle ScholarPubMed
Vertucci, C.W. and Roos, E.E. (1991) Seed moisture content, storage, viability and vigour. Seed Science Research 1, 277–279.Google Scholar
Wettlaufer, S.H. and Leopold, A.C. (1991) Relevance of Amadori and Maillard reactions to seed deterioration. Plant Physiology 97, 165–169.CrossRefGoogle ScholarPubMed
Williams, R.J. and Leopold, A.C. (1989) The glassy state in corn embryos. Plant Physiology 89, 977–981.CrossRefGoogle Scholar