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Cryopreservation of zygotic embryos and kernels of oil palm (Elaeis guineensis Jacq.)

Published online by Cambridge University Press:  19 September 2008

F. Engelmann ,
N. Chabrillange ,
S. Dussert  and
Y. Duval
F. Engelmann*
Affiliation:
ORSTOM, 911 av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
N. Chabrillange
Affiliation:
ORSTOM, 911 av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
S. Dussert
Affiliation:
ORSTOM, 911 av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
Y. Duval
Affiliation:
ORSTOM, 911 av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
*
*Present address; IPGRI, via delle Sette Chiese 142, 00145 Rome, Italy
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Abstract

In the present study, the efficiency of two cryopreservation strategies for oil palm (Elaeis guineensis Jacq.) was compared. If extracted from rehydrated kernels, 65% of the embryos desiccated to around 0.3 g H2O/g DW developed into plantlets after cryopreservation. In contrast, only 25% of embryos (0.12 g H2O/g DW) extracted from cryopreserved dry kernels developed into plantlets. However, this value was increased to 63% if kernels were partially rehydrated before freezing until the water content of embryos reached 0.3 g H2O/g DW. This study emphasizes the importance of partial rehydration of oil palm embryos before cryopreservation.

Keywords

Cryopreservationdesiccationoil palmrehydrationkernelszygotic embryos
Type
Research Papers
Information
Seed Science Research , Volume 5 , Issue 2 , June 1995 , pp. 81 - 86
Copyright
Copyright © Cambridge University Press 1995

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References

Abdelnour, A., Villalobos, V. and Engelmann, F. (1992) Cryopreservation of zygotic embryos of Coffea spp. Cryo Letters 13, 297–302.Google Scholar
Assy-Bah, B. and Engelmann, F. (1992) Cryopreservation of mature embryos of coconut (Cocos nucifera L.) and subsequent regeneration of plantlets. Cryo Letters 13, 117–126.Google Scholar
Bewley, J.D. and Black, M. (1983) Physiology and biochemistry of seeds in relation to germination. Vol. 1, Development, germination and growth. Heidelberg, Springer Verlag.Google Scholar
Chin, H.F., Krishnappillay, B. and Alang, Z.C. (1988) Cryopreservation of Veitchia and Howea palm embryos: non-development of the haustorium. Cryo Letters 9, 372–379.Google Scholar
Crowe, J.H. and Crowe, L.M. (1986) Stabilisation of membranes in anhydrobiotic organisms. pp 188–209 in Leopold, A.E. (Ed.) Membranes, metabolism and dry organisms. Ithaca, New York, Comstock Publishing Associates.Google Scholar
Dumet, D., Engelmann, F., Chabrillange, N. and Duval, Y. (1993a) Cryopreservation of standard oil palm (Elaeis guineensis Jacq.) somatic embryos involving a desiccation step. Plant Cell Reports 12, 352–355.Google Scholar
Dumet, D., Engelmann, F., Chabrillange, N. and Duval, Y. (1993b) Development of cryopreservation for oil palm somatic embryos using an improved process. Oléagineux 48, 273–278.Google Scholar
Ellis, R.H., Hong, T.D., Roberts, E.H. and Soetisna, U. (1991) Seed storage behaviour in Elaeis guineensis. Seed Science Research 1, 99–104.CrossRefGoogle Scholar
Engelmann, F. (1992) Cryopreservation of embryos. pp 281–290 in Dattée, Y., Dumas, C. and Gallais, A. (Eds) Reproductive biology and plant breeding. Berlin, Springer Verlag.Google Scholar
Grout, B.W.W., Wescott, R.J. and Pritchard, H.W. (1983) Orthodox behaviour of oil palm seeds and cryopreservation of the excised embryo for genetic conservation. Annals of Botany 52, 381–384.CrossRefGoogle Scholar
Keuls, M. (1952) The use of studentised range in connexion with analysis of variance. Euphytica 1, 112–122.CrossRefGoogle Scholar
Marin, M.L., Mafla, G., Roca, W.M. and Withers, L.A. (1990) Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen. Cryo Letters 11, 257–264Google Scholar
Monnier, M. and Leddet, C. (1980) Action du saccharose sur la résistance au gel des embryons immatures de capselle. Bulletin de la Société Botanique de France 127, 71–77.CrossRefGoogle Scholar
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.Google Scholar
Newman, D. (1939) The distribution of ranges in samples from a normal distribution expressed in terms of an independent estimate of standard deviation. Biometrika 31, 20–30.CrossRefGoogle Scholar
Normah, M.N. and Vengadasalam, M. (1992) Effects of moisture content on cryopreservation of Coffea and Vigna seeds and embryos. Cryo Letters 13, 199–208.Google Scholar
Rabéchault, H. (1968) Recherches sur la culture in vitro des embryons de palmier à huile (Elaeis guineensis Jacq.) I Effets de l'acide β-indolyl-acétique. Oléagineux 17, 757–764.Google Scholar
Rabéchault, H., Guénin, G. and Ahée, J. (1967) Absorption de l'eau par les noix de palme (Elaeis guineensis Jacq. var. Dura Becc.) I. Hydratation des différentes parties de graines amenées à des teneurs globales en eau déterminées. Cahiers ORSTOM, Série Biologie 4, 31–41.Google Scholar
Ryan, T.A. (1960) Significance tests for multiple comparison of proportions, variances and other statistics. Psychological Bulletin, 57, 318–328.Google Scholar
Stanwood, P.C. (1985) Cryopreservation of seed germplasm for genetic conservation. pp 199–226 in Kartha, K.K. (Ed.) Cryopreservation of plant cells and organs. Boca Raton, Florida, CRC Press.Google Scholar
Vallade, J. (1965) Recherches morphologiques et cytologiques sur l'embryon d'Elaeis guineensis Jacq. quiescent et en cours de germination. Mémoire d'Etudes Supérieures, Université de Dijon, 71 p.Google Scholar
Williams, R.J. and Leopold, A.C. (1989) The glassy state in corn embryos. Plant Physiology 89, 977–981.Google Scholar