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Comparative cytological and biochemical analysis of protein storage vacuoles from cotyledons and radicles of cotton seeds

Published online by Cambridge University Press:  19 September 2008

E.L. Vigil ,
A.L. Fleming ,
T. Fang ,
N. Chaney  and
W. P. Wergin
E.L. Vigil*
Affiliation:
Climate Stress Laboratory, U.S. Department of Agriculture, ARS, Beltsville, MD 20705, USA
A.L. Fleming
Affiliation:
Climate Stress Laboratory, U.S. Department of Agriculture, ARS, Beltsville, MD 20705, USA
T. Fang
Affiliation:
Climate Stress Laboratory, U.S. Department of Agriculture, ARS, Beltsville, MD 20705, USA
N. Chaney
Affiliation:
Electron Microscopy Laboratory, U.S. Department of Agriculture, ARS, Beltsville, MD 20705, USA
W. P. Wergin
Affiliation:
Electron Microscopy Laboratory, U.S. Department of Agriculture, ARS, Beltsville, MD 20705, USA
*
*Correspondence
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Abstract

Protein storage vacuoles (PSVs) from radicles and cotyledons of dry cotton seeds were isolated by differential centrifugation following homogenization in glycerol. Protein complement analysis of isolated PSVs with one dimensional SDS-PAGE gels revealed similar major storage proteins, viz. 53 and 48 kDa, with differences in lower molecular mass proteins. Radicle PSVs have apparently more 35-kDa and less 22-kDa storage protein than do cotyledon PSVs. The mineral composition of whole radicles, cotyledons and isolated PSVs from radicles and cotyledons was determined by atomic absorption spectroscopy and colorimetric elemental analyses. The concentration of calcium (Ca), magnesium (Mg), potassium (K) and phosphate (P) was lower in isolated PSVs from radicles than from cotyledons, resulting in a marked difference in the Mg/Ca and (Mg+Ca)/K ratios in PSVs from these two sources. Analysis of radicle and cotyledon tissue from dry seeds for mineral distribution with EDX and scanning electron microscopy revealed major concentrations of Mg, K and P in PSVs. These observations indicate that PSVs in radicles are similar in protein and mineral composition to PSVs in cotyledons. PSVs in radicles have the potential function as storage organelles to provide minerals and nutrients for radicle growth during imbibition and germination.

Keywords

EDXcotton seedprotein bodyatomic absorption spectroscopyradiclecotyledonSDS-PAGEelectron microscopy
Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 6 , Issue 1 , March 1996 , pp. 31 - 37
Copyright
Copyright © Cambridge University Press 1996

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