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Changes in protein composition in cellular membranes of various parts of secondary dormant cucumber seeds treated with ethanol

Published online by Cambridge University Press:  22 February 2007

Y. Sreenivasulu  and
Dilip Amritphale
Y. Sreenivasulu
Affiliation:
School of Studies in Botany, Vikram University, Ujjain (M.P.) 456 010, India
Dilip Amritphale*
Affiliation:
School of Studies in Botany, Vikram University, Ujjain (M.P.) 456 010, India
*
*Fax: 0091 734 511226, 514550 E-mail: [email protected]
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Abstract

Secondary seed dormancy in cucumber (Cucumis sativus L.) cv. Poinsett 76 could be broken with ethanol. Breakage of dormancy was accompanied by significant changes in the composition of membrane proteins in various seed parts. Compared to the intracellular membranes, a change in the protein composition of plasma membranes from the embryonic axis and cotyledons was evident early in the time-course of ethanol-induced germination of dormant seeds. While few alterations in the membrane protein composition in response to ethanol treatment occurred both in dormant and nondormant seeds, a number of changes occurred exclusively in ethanol-treated dormant seeds. Notably, a 14 kD protein in the plasma membrane from the embryonic axis and a 35 kD protein in the microsomal membranes from the perisperm-endosperm envelope disappeared in the ethanol-treated dormant seeds, but not in the ethanol-treated nondormant seeds. Also marked decrease in the content of a 23 kD protein in the plasma membrane from the cotyledons was observed in ethanol-treated dormant seeds only. It is suggested that the particular changes in cellular membrane proteins, which occurred in ethanol-treated dormant seeds much before the first visible indication of germination, might be related to dormancy breaking rather than to the germination process.

Keywords

CucumberCucumis sativus L.ethanolgerminationmembrane proteinsseed dormancy
Type
Research Article
Information
Seed Science Research , Volume 10 , Issue 1 , March 2000 , pp. 61 - 70
Copyright
Copyright © Cambridge University Press 2000

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References

Adkins, S.W., Naylor, J.M. and Simpson, G.M. (1984) The physiological basis of seed dormancy in Avena fatua V. Action of ethanol and other organic compounds. Physiologogia Plantarum 62, 18–24.CrossRefGoogle Scholar
Amritphale, D., Mukhiya, Y.K., Gupta, J.C. and Iyengar, S. (1984) Effect of storage, photoperiod and mechanical scarification on seed germination in Ocimum americanum. Physiologia Plantarum 61, 649–652.CrossRefGoogle Scholar
Amritphale, D., Dixit, S. and Singh, B. (1993) Effect of acetone on the induction and breakage of secondary dormancy in seeds of cucumber. Journal of Experimental Botany 44, 1621–1626.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1994) Seeds: Physiology of development and germination.(2nd edition) New York, Plenum Press.CrossRefGoogle Scholar
Black, M. (1996) Liberating the radicle: A case for softening-up. Seed Science Research 6, 39–42.CrossRefGoogle Scholar
Bollag, D.M. and Edelstein, S.J. (1991) Protein methods. New York, John Wiley & Sons Inc.Google Scholar
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Cohn, M.A. (1996) Operational and philosophical decisions in seed dormancy research. Seed Science Research 6, 147–153.CrossRefGoogle Scholar
Cohn, M.A. (1997) QSAR modelling of dormancy-breaking chemicals. pp. 289–295in Ellis, R.H.; Black, M.; Murdoch, A.J.; Hong, T.D. (Eds.) Basic and applied aspects of seed biology. Dordrecht, Kluwer Academic Publishers.CrossRefGoogle Scholar
Come, D. and Corbineau, F. (1989) Some aspects of metabolic regulation of seed germination and dormancy. pp. 165–179in Taylorson, R.B. (Ed.) Recent advances in the development and germination of seeds. New York, Plenum Press.CrossRefGoogle Scholar
Corbineau, F., Gouble, B., Lecat, S. and Come, D. (1991) Stimulation of germination of dormant oat (Avena sativa L.) seeds by ethanol and other alcohols. Seed Science Research 1, 21–28.CrossRefGoogle Scholar
Di Nola, L., Mischke, C.F. and Taylorson, R.B. (1990) Changes in the composition and synthesis of proteins in cellular membranes of Echinochloa crus-galli (L.) Beauv. seeds during the transition from dormancy to germination. Plant Physiology 92, 427–433.CrossRefGoogle Scholar
Dixit, S. and Amritphale, D. (1996) Very low fluence and low fluence response in the induction and inhibition of seed germination in Celosia argentea Seed Science Research 6, 43–48.CrossRefGoogle Scholar
Drapron, R. (1985) Enzyme activity as a function of water activity. pp. 171–190in Simatos, D., Moulton, J.L. (Eds.) Properties of water in foods: in relation to quality and stability. Higham, MA, Martinus Nijhoff Publication.CrossRefGoogle Scholar
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1985) Handbook of seed technology for genebanks. Volume II. Compendium of specific germination information and test recommendations. Rome, International Board for Plant Genetic Resources.Google Scholar
Foley, M.E. (1994) Temperature and water status of seed affect afterripening in wild oat (Avena fatua). Weed Science 42, 200–204.CrossRefGoogle Scholar
Footitt, S. and Cohn, M.A. (1992) Seed dormancy in red rice VIII. Embryo acidification during dormancy-breaking and subsequent germination. Plant Physiology 100, 1196–1202.CrossRefGoogle ScholarPubMed
Footitt, S., Vargas, D. and Cohn, M.A. (1995) Seed dormancy in red rice X. 13C-NMR study of the metabolism of dormancy-breaking chemicals. Physiologia Plantarum 94, 667–671.CrossRefGoogle Scholar
Hames, B.D. (1981) An introduction to polyacrylamide gel electrophoresis. pp. 1–103in Hames, B.D., Rickwood, D. (Eds) Gel electrophoresis of proteins: A practical approach. Oxford, England, IRL Press.Google Scholar
Hilhorst, H.W.M. (1998) The regulation of secondary dormancy. The membrane hypothesis revisited. Seed Science Research 8, 77–90.CrossRefGoogle Scholar
Hilhorst, H.W.M., Derkx, M.P.M. and Karssen, C.M. (1996) An integrating model for seed dormancy cycling: characterization of reversible sensitivity. pp. 341–360in Lang, G.A. (Ed.) Plant dormancy: physiology, biochemistry and molecular biology. Wallingford, CAB International.Google Scholar
Hodges, T.K. and Mills, D. (1986) Isolation of the plasma membrane. Methods in Enzymology 118, 41–54.CrossRefGoogle Scholar
ISTA (1985) International rules for seed testing. Seed Science and Technology 13, 299–513.Google Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Larsson, C., Sommarin, M. and Widell, S. (1994) Isolation of highly purified plant plasma membranes and separation of inside-out and right-side-out vesicles. Methods in Enzymology 228, 451–469.CrossRefGoogle Scholar
Peter, G. and Gendraud, M. (1996) Processes at the plasma membrane and plasmalemma ATPase during dormancy pp. 233–243in Lang, G.A. (Ed. ) Plant dormancy: physiology, biochemistry and molecular biology. Wallingford, CAB International.Google Scholar
Singh, B. (1953) Studies on the structure and development of seeds of cucurbitaceae. Phytomorphology 3, 224–239.Google Scholar
Sreenivasulu, Y. and Amritphale, D. (1998) Chemical stimulation of germination and membrane fluidity change in secondarily dormant cucumber seeds. Current Science 75, 1396–1399.Google Scholar
Taylorson, R.B. (1982) Interaction of phytochrome and other factors in seed germination. pp. 323–346in Khan, A.A. (Ed.) The physiology and biochemistry of seed development, dormancy and germination. Amsterdam, Elsevier Biomedical Press.Google Scholar
Taylorson, R.B. (1988) Anaesthetic enhancement of Echinochloa crus-galli (L.) Beauv. seed germination: possible membrane involvement. Journal of Experimental Botany 39, 50–58.CrossRefGoogle Scholar
Taylorson, R.B. and Di Nola, L. (1990) Phytochrome and membrane behaviour as influenced by temperature and anaesthetic treatment of R. crispus seeds. Seed Science and Technology 18, 549–558.Google Scholar
Taylorson, R.B. and Hendricks, S.B. (1979) Overcoming dormancy in seeds with ethanol and other anaesthetics. Planta 145, 507–510.CrossRefGoogle Scholar
Welbaum, G.E., Muthui, W.J., Wilson, J.H., Grayson, R.L. and Fell, R.D. (1995) Weakening of muskmelon perisperm envelope tissue during germination. Journal of Experimental Botany 46, 391–400.CrossRefGoogle Scholar
Wharton, D.C. and Tzagoloff, A. (1967) Cytochrome oxidase from beef heart mitochondria. Methods in Enzymology 10, 245–250.CrossRefGoogle Scholar
Wheeler, J.J. and Boss, W.F. (1987) Polyphosphoinositides are present in plasma membranes isolated from fusogenic carrot cells. Plant Physiology 85, 389–392.CrossRefGoogle ScholarPubMed
Widell, S. and Larsson, C. (1990) A critical evaluation of markers used in plasma membrane purification. pp. 17–43in Larsson, C., Moller, I.M. (Eds.) The plant plasma membrane: structure, function and molecular biology. Berlin, Springer-Verlag.Google Scholar