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Insertional RNA editing in metazoan mitochondria: The cytochrome b gene in the nematode Teratocephalus lirellus

Published online by Cambridge University Press:  01 May 1999

JACQUES R. VANFLETEREN  and
ANDY R. VIERSTRAETE
JACQUES R. VANFLETEREN
Affiliation:
Department of Biology, University of Gent, B-9000 Gent, Belgium
ANDY R. VIERSTRAETE
Affiliation:
Department of Biology, University of Gent, B-9000 Gent, Belgium

Abstract

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Analysis of the sequence of the mitochondrial cyt b gene of Teratocephalus lirellus revealed multiple frame shift events. As these sites coincided with homopolymeric tracks, sequencing was repeated and chromatograms were screened for poorly resolved peaks. No evidence for sequencing errors was found. We therefore reverse transcribed mRNA, using M-MLV reverse transcriptase and two primers for strongly conserved regions located anteriorly (CbF1: >5′-GTTTTGGAATGTTGGAAGGCTTTTAGG-3′) and posteriorly (CbR1: <5′-CCCAGATATCACCATAGCCAAATAGACG-3′) in the gene. The cDNA product was PCR amplified and sequenced, and compared with the gDNA sequences. This revealed insertions of a single U in eight sites in the mRNA sequence (Fig. 1).

Keywords

cytochrome binsertional editingmitochondrial RNANematodaTeratocephalus lirellus
Type
LETTERS TO THE EDITOR
Information
RNA , Volume 5 , Issue 5 , May 1999 , pp. 622 - 624
Copyright
© 1999 RNA Society