Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-24T09:09:26.969Z Has data issue: false hasContentIssue false
  • English
  • Français

Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins

Published online by Cambridge University Press:  28 August 2001

SUSAN K. LYMAN ,
LARRY GERACE  and
SUSAN J. BASERGA
SUSAN K. LYMAN
Affiliation:
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
LARRY GERACE
Affiliation:
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
SUSAN J. BASERGA
Affiliation:
Departments of Therapeutic Radiology and Genetics, Yale School of Medicine, New Haven, Connecticut 06520-8040, USA
Get access

Abstract

We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of the box C/D snoRNPs, and joins fibrillarin as the second such component identified and characterized in metazoans.

Keywords

genomicsnucleolusribosome biogenesissnoRNPs
Type
Research Article
Information
RNA , Volume 5 , Issue 12 , December 1999 , pp. 1597 - 1604
Copyright
1999 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)