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An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association

Published online by Cambridge University Press:  29 June 2001

STEVEN WORMSLEY
Affiliation:
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA
DMITRY A. SAMARSKY
Affiliation:
Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA
MAURILLE J. FOURNIER
Affiliation:
Departments of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01605, USA
SUSAN J. BASERGA
Affiliation:
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA
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Abstract

The U3 small nucleolar ribonucleoprotein (snoRNP) is composed of a small nucleolar RNA (snoRNA) and at least 10 proteins. The U3 snoRNA base pairs with the pre-rRNA to carry out the A0, A1, and A2 processing reactions that lead to the release of the 18S rRNA from the nascent pre-rRNA transcript. The yeast U3 snoRNA can be divided into a short 5′ domain (nt 1–39) and a larger 3′ domain (73 to the 3′ end) separated by a stretch of nucleotides called the hinge region (nt 40–72). The sequences required for pre-rRNA base pairing are found in the 5′ domain and hinge region whereas the 3′ domain is largely covered with proteins. Mpp10p, one of the protein components unique to the U3 snoRNP, plays a role in processing at the A1 and A2 sites. Because of its critical role in U3 snoRNP function, we determined which sequences in the U3 snoRNA are required for Mpp10p association. Unlike fibrillarin and all the previous U3 snoRNP components studied in this manner, sequences in the 3′ domain are not sufficient for Mpp10p association. Instead, a conserved sequence element in the U3 snoRNA hinge region is required, placing Mpp10p near the 5′ domain that carries out the pre-rRNA base-pairing interactions in the functional center of the U3 snoRNP.

Type
Research Article
Copyright
2001 RNA Society

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