Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-27T17:55:22.495Z Has data issue: false hasContentIssue false
  • English
  • Français

Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels

Published online by Cambridge University Press:  01 November 1999

RITA DAS  and
ROBIN REED
RITA DAS
Affiliation:
Harvard Medical School, Boston, Massachusetts 02115, USA
ROBIN REED
Affiliation:
Harvard Medical School, Boston, Massachusetts 02115, USA
Get access

Abstract

A great deal of progress in elucidating the mechanisms of spliceosome assembly has been achieved by analyzing the A, B, and C spliceosomal complexes on native polyacrylamide gels. In contrast, progress in understanding the earliest spliceosomal complex E has been hampered because this complex dissociates on native gels and is difficult to detect by other methods. Here we report conditions for resolving the spliceosomal complex E using a native horizontal agarose mini-gel system. This system also provides a simple alternative to polyacrylamide gels for resolving the ATP-dependent spliceosomal complexes.

Keywords

E complexnative gelspliceosomespliceosome assemblysplicing
Type
METHODS
Information
RNA , Volume 5 , Issue 11 , November 1999 , pp. 1504 - 1508
Copyright
1999 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)