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Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins

Published online by Cambridge University Press:  28 August 2001

SUSAN K. LYMAN ,
LARRY GERACE  and
SUSAN J. BASERGA
SUSAN K. LYMAN
Affiliation:
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
LARRY GERACE
Affiliation:
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
SUSAN J. BASERGA
Affiliation:
Departments of Therapeutic Radiology and Genetics, Yale School of Medicine, New Haven, Connecticut 06520-8040, USA
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Abstract

We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of the box C/D snoRNPs, and joins fibrillarin as the second such component identified and characterized in metazoans.

Keywords

genomicsnucleolusribosome biogenesissnoRNPs
Type
Research Article
Information
RNA , Volume 5 , Issue 12 , December 1999 , pp. 1597 - 1604
Copyright
1999 RNA Society

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