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Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits

Published online by Cambridge University Press:  01 January 1998

HALLER IGEL ,
SANDRA WELLS ,
RHONDA PERRIMAN  and
MANUEL ARES Jr.
HALLER IGEL
Affiliation:
Center for the Molecular Biology of RNA, Biology Department, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, California 95064, USA
SANDRA WELLS
Affiliation:
Center for the Molecular Biology of RNA, Biology Department, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, California 95064, USA
RHONDA PERRIMAN
Affiliation:
Center for the Molecular Biology of RNA, Biology Department, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, California 95064, USA
MANUEL ARES Jr.
Affiliation:
Center for the Molecular Biology of RNA, Biology Department, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, California 95064, USA
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Abstract

Human SAP 49, a subunit of the multimeric splicing factor 3b (SF3b), contains two RNA recognition motifs (RRMs) and binds another SF3b subunit called SAP 145, whose yeast homologue is CUS1. Here we show that the predicted yeast open reading frame YOR319w (HSH49) encodes an essential yeast splicing factor. Using bacterially expressed proteins, we find that yeast HSH49 binds CUS1. Mutations that alter putative RNA-binding residues of either HSH49 RRM are lethal in vivo, but do not prevent binding to CUS1 in vitro, suggesting that the predicted RNA-binding surfaces of HSH49 are not required for interaction with CUS1. In vivo interaction tests show that HSH49 and CUS1 associate primarily through the N-terminal RRM of HSH49. Recombinant HSH49 protein has a general RNA-binding activity that does not require CUS1. The parallels in structure and interaction between two SF3b subunits from yeast implies that the mechanism of SF3b action is highly conserved.

Keywords

RNA-binding proteinsRNA processingSaccharomyces cerevisiaeSAP 49
Type
Research Article
Information
RNA , Volume 4 , Issue 1 , January 1998 , pp. 1 - 10
Copyright
© 1998 RNA Society

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