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Radiocarbon Dating and Stable Isotopic Analysis of Insect Chitin from the Rancho La Brea Tar Pits, Southern California

Published online by Cambridge University Press:  28 January 2016

A R Holden*
Affiliation:
Richard Gilder Graduate School at the American Museum of Natural History, 79th at Central Park West, New York, NY 10024, USA. Entomology Section, Natural History Museum of Los Angeles County, 900 Exposition Boulevard, Los Angeles, CA 90007, USA. George C. Page Museum at the La Brea Tar Pits, 5801 Wilshire Boulevard, Los Angeles, CA 90036, USA.
J R Southon
Affiliation:
Department of Earth System Science, University of California-Irvine, Irvine, CA 92697, USA.
*
*Corresponding author. Email: [email protected].

Abstract

This paper presents the first successful methods for accelerator mass spectrometry (AMS) dating of asphalt-impregnated insect chitin from the Rancho La Brea Tar Pits in southern California. A persistent problem with stratigraphic correlation at this site is that asphalt flows are characteristically intermittent and are really discontinuous, which can result in mixing fossils of quite different ages. Direct 14C dating of specimens circumvents this difficulty but requires a pretreatment method that can produce dates from relatively small samples (<10 mg) of insect cuticle, while successfully removing residual asphalt and sample preparation solvents as well as soil carbon contamination. 14C dating accuracy was verified by comparing dates on insect chitin with ages for seeds and twigs compacted during a rapid entrapment event within a separately dated skull of the Western Camel, Camelops hesternus Leidy. All dates fell within a relatively narrow range of ~40,000–44,000 14C yr BP, suggesting that such methods can be used with confidence on other insect material from this site. Insects are often superior paleoenvironmental indicators for establishing precise data points for climate fluctuations. This is because their lifecycles and present-day climate-restricted geographic distributions are well documented, and unlike migrating mammals and birds, insects offer crucial information about the local environment. Our results are therefore potentially significant for studies of paleoecological and paleoclimatic change within the Los Angeles Basin and coastal southern California, as well as reconstruction of entrapment events at Rancho La Brea.

Type
Research Article
Copyright
© 2016 by the Arizona Board of Regents on behalf of the University of Arizona 

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References

REFERENCES

Andersen, SA. 2010. Insect cuticular sclerotization: a review. Insect Biochemistry and Molecular Biology 40(3):166178.CrossRefGoogle ScholarPubMed
Brooks, SJ, Langdon, PG. 2014. Summer temperature gradients in northwest Europe during the Late glacial to early Holocene transition (15–8 ka BP) inferred from chironomid assemblages. Quaternary International 341:8090.Google Scholar
Coope, GR. 2009. Beetles as Quaternary and Late Tertiary climate indicators. In: Gornitz V, editor. Encyclopedia of Paleoclimatology and Ancient Environments . Dordrecht: Springer. p 9091.Google Scholar
DeNiro, MJ, Epstein, S. 1981. Influence of diet on the distribution of nitrogen isotopes in animals. Geochimica et Cosmochimica Acta 45(3):341351.Google Scholar
Elias, SA. 1994. Quaternary Insects and Their Environments. Washington, DC: Smithsonian Institution Press.Google Scholar
Elias, S. 2010. Advances in Quaternary Entomology. Amsterdam: Elsevier.Google Scholar
Friscia, AR, Van Valkenburgh, B, Spencer, L, Harris, JM. 2008. Chronology and spatial distribution of large mammal bones in Pit 91, Rancho La Brea. Palaios 23(1):3542.Google Scholar
Fuller, BT, Fahrni, SM, Harris, JM, Farrell, AB, Coltrain, JB, Gerhart, LM, Ward, JK, Taylor, RE, Southon, JR. 2014. Ultrafiltration for asphalt removal from bone collagen for radiocarbon dating and isotopic analysis of Pleistocene fauna at the tar pits of Rancho La Brea, Los Angeles, California. Quaternary Geochronology 22:8598.Google Scholar
Goedkoop, W, Akerblom, N, Demandt, MH. 2006. Trophic fractionation of carbon and nitrogen stable isotopes in Chironomus riparius reared on food of aquatic and terrestrial origin. Freshwater Biology 51(5):878886.Google Scholar
Goual, L. 2012. Petroleum asphaltenes, in crude oil emulsions - composition stability and characterization, 27-42. In: Abdul-Raouf M, editor. Rijecka, Croatia: Intech. Available from www.intechopen.com/books/crude-oil-emulsions-composition-stability-and-characterization. Accessed 21 June 2015.Google Scholar
Hein, O, Schilder, J, van Hardenbroek, M. 2012. Stable isotope analysis of fossil chironomids as an approach to environmental reconstruction: state of development and future challenges. Fauna Norvegica 31:718.Google Scholar
Heusser, LE. 2000. Rapid oscillations in western North America vegetation and climate during oxygen isotope stage 5 inferred from pollen data from Santa Barbara Basin (Hole 893A). Palaeogeography, Palaeoclimatology, Palaeoecology 161(3):407421.CrossRefGoogle Scholar
Hodgins, GWL, Thorpe, JL, Coope, GR, Hedges, REM. 2001. Protocol development for purification and characterization of sub-fossil insect chitin for stable isotope analysis and radiocarbon dating. Radiocarbon 43(2A):199208.CrossRefGoogle Scholar
Karlson, P, Sekeri, K, Marmaras, VI. 1969a. Die aminosaurezusamensetzung verscheidener proteinfraktionen aus der cuticula von Calliphora Erythrocephala. Journal of Insect Physiology 15:319323.Google Scholar
Karlson, P, Sekeri, K, Richards, AG, Richards, PA. 1969b. The amino acid composition of various types of cuticle of Limuluus polyphemus . Journal of Insect Physiology 15(3):495507.CrossRefGoogle Scholar
Miller, SE. 1983. Late Quaternary insects of Rancho La Brea and McKittrick, California. Quaternary Research 20(1):90104.CrossRefGoogle Scholar
Mullins, OC, Sheu, EY, Hammami, A, Marshall, AG. 2007. Asphaltenes, Heavy Oils, and Petroleomics. New York: Springer.CrossRefGoogle Scholar
Muzzarelli, RAA. 2011. Chitin nanostructures in living organisms. In: Gupta N, editor. Chitin Formation and Diagenesis. New York: Springer. p 135.Google Scholar
O’Keefe, FR, Fet, EV, Harris, JM. 2009. Compilation, calibration and synthesis of faunal and floral radiocarbon dates, Rancho La Brea, California. Contributions in Science, Natural History Museum of Los Angeles County 518:116.Google Scholar
Panagiotakopulu, E, Higham, TFG, Buckland, PC, Tripp, JA. 2015. AMS dating of insect chitin – a discussion of new dates, problems and potential. Quaternary Geochronology 27:2232.Google Scholar
Sadava, DE, Hillis, DM, Heller, HC, Berenbaum, M. 2009. Life: The Science of Biology Volume 2. New York: Macmillan.Google Scholar
Santos, GM, Southon, JR, Griffin, S, Beaupre, SR, Druffel, ER. 2007. Ultra small-mass 14C-AMS sample preparation and analysis at the KCCAMS Facility. Nuclear Instruments and Methods in Physics Research B 259(1):293330.Google Scholar
Schimmelmann, A, DeNiro, MJ. 1986a. Stable isotopic studies on chitin, measurements on chitin/chitosan isolates and D-glucosamine hydrochloride from chitin. In: Muzzarelli R, Jeuniaux C, Gooday GW, editors. Chitin in Nature and Technology. New York: Plenum. p 357364.CrossRefGoogle Scholar
Schimmelmann, A, DeNiro, MJ. 1986b. Stable isotopic studies on chitin II: the 13C/12C and 15N/14N ratios in arthropod chitin. Contributions in Marine Science 29:113130.Google Scholar
Southon, JR, Santos, G, Druffel-Rodriguez, K, Druffel, E, Trumbore, S, Xu, XM, Griffin, S, Ali, S, Mazon, M. 2004. The Keck Carbon Cycle AMS laboratory, University of California, Irvine: initial operation and a background surprise. Radiocarbon 46(1):4149.Google Scholar
Stuiver, M, Polach, HA. 1977. Discussion: reporting of 14C data. Radiocarbon 19(1):355363.Google Scholar
Templeton, BC. 1964. The fruits and seeds of the Rancho La Brea Pleistocene deposits [PhD dissertation]. Corvallis: Oregon State University.Google Scholar
Tripp, JA, Higham, TFG. 2011. Radiocarbon dating of chitin. In: Gupta NS, editor. Chitin Formation and Diagenesis. Netherlands: Springer. p 6180.Google Scholar
Tripp, JA, Higham, TFG, Hedges, REM. 2004. A pretreatment procedure for the AMS radiocarbon dating of sub-fossil insect remains. Radiocarbon 46(1):147154.Google Scholar
Verbruggen, F, Heiri, O, Reichart, GJ, Lotter, AF. 2011. Chironomid δ18O as a proxy for past lake water δ18O: a Lateglacial record from Rotsee (Switzerland). Quaternary Science Reviews 29(17--18):22712279.CrossRefGoogle Scholar
Walker, MJC, Bryant, C, Coope, GR, Harkness, DD, Lowe, JJ, Scott, EM. 2001. Towards a radiocarbon chronology of the Late-Glacial: sample selection strategies. Radiocarbon 43(2B):10071019.CrossRefGoogle Scholar
Ward, JK, Harris, JM, Cerling, TE, Wiedenhoeft, A, Lott, M, Dearing, MD, Coltrain, JB, Ehleringer, JR. 2005. Carbon starvation in glacial trees recovered from the La Brea Tar Pits, southern California. Proceedings of the National Academy of Sciences of the USA 102(3):690694.Google Scholar
Wooler, MJ, Francis, D, Fogel, ML, Miller, U, Walker, IR, Wolfe, AP. 2004. Quantitative paleotemeperature estimates from δ18O of chironomid head capsules preserved in arctic lake sediments. Journal of Paleolimnology 31(3):267274.CrossRefGoogle Scholar