The description of the molecular processes which underlie visual excitation is the fundamental problem in understanding vision at the level of a single photoreceptor. Thus far only a general outline of photoreceptor function has emerged with little known about actual biochemical and biophysical mechanisms.

The concept that the liquid crystalline or mesomorphic condition was of importance to biological systems is a relatively old idea. Thus Bernal (1933) when discussing the different types of arrangements of molecules in liquid crystals commented ‘Such structures belong to the liquid crystal as a unit and not to its molecules which may be replaced by others without destroying them and they persist in spite of the complete fluidity of the substance. These are just the properties to be required for a degree of organization between that of the continuous substance, liquid or crystalline solid and even the simplest living cell.’ Stewart (1961) some thirty years later also stated that ‘It is this property – the combination of flow and lability with a preferred and relatively stable molecular orientation – that makes the mesomorphic (i.e. liquid crystal) phase uniquely appropriate to the structure of protoplasm and living tissue.’

Fluorescence has been used in biochemical studies for many years but only recently has the information content and the practical applicability of the fluorescence method been fully realized.

Following the early studies of Newton (1954) and Weber (11954) and after the initial utilization of fluorescent probes by Chance and coworkers (Azzi et al. 1969) and Tasaki et al. (1968), in the study of membranes, the use of fluorescence to provide structural information at microscopic or molecular levels in biological membranes has become widespread. widespread. The application of the fluorescence technique to biological systems has progressed parallel to the development of a theoretical basis for fluorescence data interpretation and the synthesis of a large number of fluorescent probes, organic molecules having fluorescence characteristics that are dependent on their environment.