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PNA-nucleic acid complexes. Structure, stability and dynamics

Published online by Cambridge University Press:  17 March 2009

Magdalena Eriksson  and
Peter E. Nielsen
Magdalena Eriksson
Affiliation:
Center for Biomolecular Recognition, The Panum Institute, Department of Biochemistry B, Blegdamsvej 3c, DK-2200 Copenhagen N, Denmark
Peter E. Nielsen
Affiliation:
Center for Biomolecular Recognition, The Panum Institute, Department of Biochemistry B, Blegdamsvej 3c, DK-2200 Copenhagen N, Denmark
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Extract

Growing interest in gene targeting drugs has inspired the development of a multitude of nucleic acid analogues, many of which feature substitutions in the phosphodiester moiety of the backbone (reviewed by Mesmaeker et al. 1995 and Nielsen, 1995). Peptide nucleic acid (PNA) is an example of a more radical redesign of DNA. The entire sugar-phosphate backbone is substituted by a chain of peptide-like N-(2-aminoethyl)glycine units so that an achiral and uncharged DNA-mimic is obtained (Fig. 1; Nielsen et al. 1991). The synthesis is based on standard peptide chemistry (Christensen et al. 1995) and has been automated. PNA can relatively easily be modified to include modifications of the backbone as well as of the bases (Hyrup & Nielsen, 1996). PNA is chemically stable and, in contrast to natural nucleic acids and peptides, PNA is expected to remain intact in living cells since it is not a substrate for natural hydrolytic enzymes and is not degraded by cell extracts (Demidov et al. 1994).

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 29 , Issue 4 , December 1996 , pp. 369 - 394
Copyright
Copyright © Cambridge University Press 1996

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