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Neutron scattering studies of biological macromolecules in solution

Published online by Cambridge University Press:  17 March 2009

G. G. Kneale ,
J. P. Baldwin  and
E. M. Bradbury
G. G. Kneale
Affiliation:
Biophysics Laboratory, Portsmouth Polytechnic, Portsmouth, U.K.
J. P. Baldwin
Affiliation:
Biophysics Laboratory, Portsmouth Polytechnic, Portsmouth, U.K.
E. M. Bradbury
Affiliation:
Biophysics Laboratory, Portsmouth Polytechnic, Portsmouth, U.K.
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Extract

Small-angle neutron scattering studies of biological macromolecules have developed rapidly in recent years due to the advent of high neutron-flux reactors,† efficient position-sensitive area detectors for neutrons (Allemand et al. 1975) and novel design of instruments (Ibel, 1976). At the same time position-sensitive detectors for X-rays (Gabriel & Dupont, 1972) have revitalized the small-angle X-ray scattering field (Kratky & Pilz, 1972; Luzzati et al. 1976) and it is now becoming clear that neutrons and X-rays can provide complementary structural information on biological complexes.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 10 , Issue 4 , November 1977 , pp. 485 - 527
Copyright
Copyright © Cambridge University Press 1977

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References

REFERENCES

Allemand, R.Bourdet, J.Roudaut, E.Convert, P.Ibel, K.Jacobe, J.Cotton, J. P. & Farnoux, B. (1975). Position sensitive detectors for neutron diffraction. Nucl. Instrum. Meth. 126, 29–42.CrossRefGoogle Scholar
Bacon, G. E. (1963). Applications of Neutron Diffraction in Chemistry. Pergamon Press.Google Scholar
Bradbury, E. M.Baldwin, J. P.Carpenter, B. G.Hjelm, R. P.Hancock, R. & Ibel, K. (1975). Neutron-scattering studies of chromatin. Brookhaven Symp. Biol. 27, IV, 97–117.Google Scholar
Crichton, R. R.Engelman, D. M.Haas, J.Koch, M. H. J.Moore, P. B.Parfait, R. & Stuhrmann, H. B. (1977). A contrast variation study of specifically deuterated E. coli ribosomal subunits. (In the Press.)Google Scholar
Daboll, H. F.Crespi, H. L. & Katz, J. J. (1962). Mass cultivation of algae in pure heavy water. Biotechnol. Bioeneng 4, 281–97.CrossRefGoogle Scholar
Debye, P. (1915). Scattering from non-crystalline substances. Annln Phys., Leipzig 46, 809–23.CrossRefGoogle Scholar
Engelman, D. M. & Moore, P. B. (1972). A new method for the determination of biological quaternary structure by neutron scattering. Proc. natn. Acad. Sci. U.S.A. 69, 1997–9.CrossRefGoogle ScholarPubMed
Engelman, D. M.Moore, P. B. & Schoenborn, B. P. (1975). Protein pair distance determination in the 30 S ribosomal subunit of E. coli. Brookhaven Symp. Biol. 27 IV, 20–37.Google Scholar
Fischback, F. A. & Anderegg, J. W. (1965). An X-ray scattering study of Ferritin and Apoferritin. J. molec. Biol. 14, 458–73.CrossRefGoogle Scholar
Gabriel, A. & Dupont, Y. (1972). Position sensitive proportional detector for X-ray crystallography. Rev. Scient. Instrum. 43, 1600–2.CrossRefGoogle ScholarPubMed
Glatter, O. (1974). A new iterative method for collimation correction in small-angle scattering. J. Appl. Crystallogr. 7, 147–53.CrossRefGoogle Scholar
Guinier, A. (1963). X-ray Diffraction in Crystals, Imperfect Crystals and Amorphous Bodies. Freeman, W. H..Google Scholar
Guinier, A. & Fournet, G. (1955). Small Angle Scattering of X-rays. New York: Wiley.Google Scholar
Harrison, S. C. (1969). Structure of tomato bushy stunt virus. J. Molec. Biol. 42, 457–83.CrossRefGoogle ScholarPubMed
Hjelm, R. P.Kneale, G. G.Suau, P.Baldwin, J. P.Bradbury, E. M. & Ibel, K. (1977). Small-angle neutron scattering studies of chromatin subunits in solution. Cell 10, 139–51.CrossRefGoogle ScholarPubMed
Hoppe, W. (1972). A new X-ray method for the determination of the quaternary structure of protein complexes. Israel J. Chem. 10, 321–33.CrossRefGoogle Scholar
Hoppe, W. (1973). The label triangulation method and the mixed isomorphous replacement principle. J. molec. Biol. 78, 581–5.CrossRefGoogle ScholarPubMed
Hoppe, W.May, R.Stockel, P.Lorenz, S.Erdmann, V. A.Wittmann, H. G.Crespi, H. L.Katz, J. J. & Ibel, K. (1975). Neutron scattering measurements with the label triangulation method on the 50 S subunit of E. coli ribosomes. Brookhaven Symp. Biol. 27 IV, 38–48.Google Scholar
Ibel, K. (1976). The neutron small-angle camera DII at the high-flux reactor, Grenoble. J. appl. Crystallogr. 9, 296–309.CrossRefGoogle Scholar
Ibel, K. & Stuhrmann, H. B. (1975). Comparison of neutron and X-ray scattering of dilute myoglobin solutions. J. molec. Biol. 2, 255–66.CrossRefGoogle Scholar
Jacrot, B., Chauvin, C. & Witz, J. (1977). Comparative neutron small-angle scattering study of small spherical RNA viruses. Nature, Lond. 266, 417–20.CrossRefGoogle ScholarPubMed
Kratky, O. & Pilz, I. (1972). Recent advances and applications of diffuse X-ray small-angle scattering on biopolymers in dilute solution. Q. Rev. Biophys. 5, 481–537.CrossRefGoogle Scholar
Luzzati, V.Tardieu, A.Mateu, L. & Stuhrmann, H. B. (1976) Structure of human serum lipoproteins in solution. J. Molec. Biol. 101, 115–27.CrossRefGoogle ScholarPubMed
Marguerrie, G. & Stuhrmann, H. B. (1976). A neutron small-angle scattering study of bovine fibrinogen. J. Molec. Biol. 102, 143–56.CrossRefGoogle Scholar
May, R. (1977). Communicated at the ‘Workshop on Small Angle Scattering of X-rays and Neutrons in Biology’, Villard-de-Lans.Google Scholar
Miller, A.Brodsky, Doyle B.Hulmes, D. J. S.Jenkins, G. T. & White, J. W. (1975). Neutron scattering by collagen. Brookhaven Symp. Biol. 27 III, 86–100.Google Scholar
Moore, P. B. & Engelman, D. M. (1977). Communicated at the ‘Workshop on Small Angle Scattering of X-rays and Neutrons in Biology’, Villard-de-Lans.Google Scholar
Moore, P. B. & Engelman, D. M. (1975). The production of deuterated E. coli. Brookhaven Symp. Biol. 27 V, 12–23.Google Scholar
Moore, P. B., Engelman, D. M. & Schoenborn, B. P. (1974). Asymmetry in the 50 S ribosomal subunit of E. coli. Proc. natn. Acad. Sci. U.S.A. 71, 172–6.CrossRefGoogle Scholar
Moore, P. B.Engelman, D. M. & Schoenborn, B. P. (1975). A neutron scattering study of the distribution of protein and RNA in the 30 S ribosomal subunit of E. coil. J. Molec. Biol. 91, 101–20.CrossRefGoogle Scholar
Noll, M. (1974). Subunit structure of chromatin. Nature, Lond. 251, 249–51.CrossRefGoogle ScholarPubMed
Pardon, J. F. (1977). Communicated at the ‘Workshop on Small Angle Scattering of X-rays and Neutrons in Biology’, Villard-de-Lans.Google Scholar
Pardon, J. F.Worcester, D. L.Wooley, J. C.Tatchell, K.Van Holde, K. E. & Richards, B. M. (1975). Low-angle neutron scattering from chromatin subunit particles. Nucl. Acids Res. 2, 2163–76.CrossRefGoogle ScholarPubMed
Pollard, H.Scanu, A. M. & Taylor, E. W. (1969). On the geometrical arrangement of the protein subunits of human serum low-density lipoprotein. Proc. natn. Acad. Sci. U.S.A. 64, 304–10.CrossRefGoogle ScholarPubMed
Porod, G. (1951). Die Rontgenkleinwinkelstreuung von dichtgepackten kolloiden Systemen. Kolloidzeitschrift 124, 83–114.Google Scholar
Randall, J.Starling, D.Baldwin, J. P. & Ibel, K. (1975). The scattering of neutrons by catalase. Brookhaven Symp. Biol. 27 IV, 78–96.Google Scholar
Sperling, L. (1977). Communicated at the ‘Workshop in Small Angle Scattering of X-rays and Neutrons in Biology’, Villard-de-Lans.Google Scholar
Stockel, P. (1977). Communicated at the ‘Workshop in Small Angle Scattering of X-rays and Neutrons in Biology’, Villard-de-Lans.Google Scholar
Stuhrmann, H. B. (1974). Neutron small-angle scattering of biological macromolecules in solution. J. appl. Crystallogr. 7, 173–8.CrossRefGoogle Scholar
Stuhrmann, H. B. (1975). Small-angle scattering of proteins in solution. Brookhaven Symp. Biol. 27 IV, 3–19.Google Scholar
Stuhrmann, H. B. & Kirste, R. G. (1965). Z. phys. Chem. 46, 247–50.CrossRefGoogle Scholar
Stuhrmann, H. B. & Duee, E. D. (1975). The determination of the scattering density distribution of polydisperse solutions by contrast variation: a neutron scattering study of ferritin. J. appl. Crystallogr. 8, 538–42.CrossRefGoogle Scholar
Stuhrmann, H. B. & Fuess, M. (1976). A neutron small-angle scattering study of hen egg-white lysozyme. Acta crystattogr. A 32, 67–74.CrossRefGoogle Scholar
Stuhrmann, H. B.Tardieu, A.Mateu, L.Sardet, C.Luzzati, V.Aggerbeck, L. & Scanu, A. M. (1975). Neutron scattering study of human serum low density lipoprotein. Proc. natn. Acad. Sci. U.S.A. 6 2270–3.CrossRefGoogle Scholar
Stuhrmann, H. B.Haas, J.Ibel, K.Dewolf, B.Koch, M. H. J.Parfait, R. & Crichton, R. R. (1976 a). New low resolution model for 50 S subunit of E. coli ribosomes. Proc. natn. Acad. Sci. U.S.A. 73, 2379–83.CrossRefGoogle Scholar
Stuhrmann, H. B.Hass, J.Ibel, K.Koch, M. H. J.Parfait, R.Dewolf, B. & Crichton, R. R. (1976 b). Ber. Bunsen. Ges. Phys. Chem. 80, 1230–1.Google Scholar
Stuhrmann, H. B.Hass, J.Ibel, K.Koch, M. H. J. & Crichton, R. R. (1976 c). Low angle neutron scattering of ferritin studied by contrast variation. J. molec. Biol. 100, 399–413.CrossRefGoogle ScholarPubMed
Stuhrmann, H. B.Koch, M. H. J.Parfait, R.Hass, J.Ibel, K. & Crichton, R. R. (1977). Shape of the 50 S subunit of E. coli ribosomes. Proc. natn. Acad. Sci. U.S.A. (In the Press.)Google Scholar
Suau, P.Kneale, G. G.Braddock, G. W.Baldwin, J. P. & Bradbury, E. M. (1977). Low resolution model for the chromatin core particle by neutron scattering. Nucl. Acid Res. (in the Press).CrossRefGoogle Scholar
Tardieu, A.Mateu, L.Sardet, C.Weiss, B.Luzzati, V.Aggerbeck, L. & Scanu, A. M. (1976). Structure of human serum lipoproteins in solution. J. molec. Biol. 101, 129–53.CrossRefGoogle ScholarPubMed
Thomas, J. O. & Kornberg, R. D. (1975). Octamer of histones in chromatin and free in solution. Proc. natn. Acad. Sci. U.S.A. 72, 2626–30.CrossRefGoogle ScholarPubMed
Traub, P.Mizushima, S.Lowery, C. V. & Nomura, M. (1971). Reconstitution of ribosomes from sub-ribosomal components. Meth. Enzym. 20 c, 391–407.CrossRefGoogle Scholar
Van Holde, K. E. & Isenberg, I. (1975). Histone interactions and chromatin structure. Acc. Chem. Res. 8, 327–35.CrossRefGoogle Scholar
Worcester, D. L. (1975). Neutron diffraction studies of vertebrate striated muscle. Brookhaven Symp. Biol. 27 III, 101–14.Google Scholar
Worcester, D. L., Gillis, J. M., O'brien, E. J. & Ibel, K. (1975). Neutron diffraction studies of biological membranes and membrane components. Brookhaven Symp. Biol. 27 III, 37–57.Google Scholar