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Microscopic behaviour of DNA during electrophoresis: electrophoretic orientation

Published online by Cambridge University Press:  17 March 2009

Bengt Nordén ,
Christer Elvingson ,
Mats Jonsson  and
Björn Åkerman
Bengt Nordén
Affiliation:
Department of Physical Chemistry, Chalmers University of Technology, 5–412 96 Gothenburg, Sweden
Christer Elvingson
Affiliation:
Department of Physical Chemistry, Chalmers University of Technology, 5–412 96 Gothenburg, Sweden
Mats Jonsson
Affiliation:
Department of Physical Chemistry, Chalmers University of Technology, 5–412 96 Gothenburg, Sweden
Björn Åkerman
Affiliation:
Department of Physical Chemistry, Chalmers University of Technology, 5–412 96 Gothenburg, Sweden
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Extract

The study of the behaviour of DNA when subjected to electric fields poses several intriguing problems of fundamental physico-chemical importance. Electric field (Kerr effect) orientation of DNA in free solution as well as migration of DNA in gel electrophoresis are two well-established, but so far rather separate, research fields. Whereas the first one has been generally concerned with basic structural and dynamical properties of DNA (Charney, 1988), the second is closely related to techniques of molecular biology (for a review on DNA electrophoresis, see stellwagen 1987).

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 24 , Issue 2 , May 1991 , pp. 103 - 164
Copyright
Copyright © Cambridge University Press 1991

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