Solution assembly of the pseudo-high affinity and intermediate affinity interleukin-2 receptor complexes

Abstract

The high affinity interleukin-2 receptor is composed of three cell surface subunits, IL-2Rα, IL-2Rβ, and IL-2Rγ. Functional forms of the IL-2 receptor exist, however, that enlist only two of the three subunits. On activated T-cells, the α- and β-subunits combine as a preformed heterodimer (the pseudo-high affinity receptor) that serves to capture IL-2. On a subpopulation of natural killer cells, the β- and γ-subunits interact in a ligand-dependent manner to form the intermediate affinity receptor site. Previously, we have demonstrated the feasibility of employing coiled-coil molecular recognition for the solution assembly of a heteromeric IL-2 receptor complex. In that study, although the receptor was functional, the coiled-coil complex was a trimer rather than the desired heterodimer. We have now redesigned the hydrophobic heptad sequences of the coiled-coils to generate soluble forms of both the pseudo-high affinity and the intermediate affinity heterodimeric IL-2 receptors. The properties of these complexes were examined and their relevance to the physiological IL-2 receptor mechanism is discussed.