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Locating and identifying posttranslational modifications by in-source decay during MALDI-TOF mass spectrometry

Published online by Cambridge University Press:  07 March 2001

JOHN J. LENNON  and
KENNETH A. WALSH
JOHN J. LENNON
Affiliation:
Department of Biochemistry, University of Washington, Seattle, Washington 98195-7350 Current address: Large Scale Biology Corp., 9620 Medical Center Drive, Rockville, Maryland 20850-3338.
KENNETH A. WALSH
Affiliation:
Department of Biochemistry, University of Washington, Seattle, Washington 98195-7350
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Abstract

A technique is described for identifying and locating posttranslational modifications (PTMs) in peptides and proteins of known sequence by interpretation of cn ion signals generated by in-source decay during delayed ion extraction in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Sites of phosphorylation in seven synthetic peptides were determined, as was the location of both the heme group and N,N,N-trimethyllysine in yeast cytochrome c. A semi-automated data analysis process facilitates the identification of segments of the sequence on each side of the PTM, permitting its placement at the junction of the segments and definition of the added mass. A graphical display facilitates illustration of both the location and mass of the PTM.

Keywords

delayed ion extractionin-source decayMALDIposttranslational modificationsprotein sequencing by MALDI
Type
Research Article
Information
Protein Science , Volume 8 , Issue 11 , November 1999 , pp. 2487 - 2493
Copyright
© 1999 The Protein Society

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