Abundant evidence exists to suggest that increased oxidative stress may contribute to the neuropathology of age-related brain disorders such as Alzheimer's and Parkinson's diseases(Reference Crossthwaite, Hasan and Williams1, Reference Zhang, Dawson and Dawson2). Recently there has been an increased interest in the potential of dietary-derived phytochemicals to protect against neuronal damage associated with aging and neurodegenerative disorders. Astaxanthin, a natural carotenoid, is mainly used as a pigmentation source in aquaculture but increasing studies are proving its biological activity in protecting cell against oxidative stress with very interesting potential health applications such as in the treatment of degenerative diseases(Reference Chang, Chen and Chiou3). The major objective of this project was to investigate the ability of astaxanthin (synthetic v. natural) to protect against H2O2-induced neurotoxicity via its interactions with neuronal signalling pathways.
Fig. 1. Protective effect of astaxanthin against H2O2-induced neurotoxicity.
Both natural and synthetic astaxanthin did not induce neuronal injury as assessed by the Alamar blue assay 24 h post-exposure. Exposure of cortical neurons to H2O2 (75 μM, 1 h), resulted in a significant decrease in neuronal viability (−48%, P<0.01). However, treatment with astaxanthin (0.01–3 μM, 18 h) significantly protected neuronal cells against H2O2-induced toxicity. Interestingly the synthetic astaxanthin did not protect the neurons at the highest concentration of astaxanthin tested (3 μM) while the natural astaxanthin showed a further increase in the protective effect (Fig. 1). The mechanism by which astaxanthin inhibited neuronal death was found to be linked to its ability to induce the activation of both Akt/PKB and the ERK1/2 pathways. Indeed, assessment of phospho-Akt revealed a bell-shape modulation with the greatest activation observed at 0.1 μM and 0.3 μM for natural and synthetic astaxanthin, respectively (1.3 fold increase, P<0.05). Interestingly, while natural astaxanthin exerted a dose-dependent activation in p-ERK1 (extracellular signal-regulated kinases 1), synthetic astaxanthin differentially modulated p-EKR2 (2 fold increase, P<0.05). Such discrepancies may be due to differences in enantiomeric composition between the synthetic astaxanthin which is a mixture of four isomers (1:2(meso):1), and the natural which is produced as a pure enantiomer (3R, 3′R) by the yeast Phaffia rhodozyma. The protective effects of astaxanthin against neurotoxins-induced toxicity will help shed light on their mechanism of neuroprotection.
