Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-03T03:06:02.018Z Has data issue: false hasContentIssue false

Modulation of adipokines in an overweight/obese population by a pomegranate extract

Published online by Cambridge University Press:  19 October 2012

K. P. Conroy
Affiliation:
School of Health Sciences, Queen Margaret University, Edinburgh, Scotland, EH21 6UU, UK
M. Warnock
Affiliation:
School of Health Sciences, Queen Margaret University, Edinburgh, Scotland, EH21 6UU, UK
I. Davidson
Affiliation:
School of Health Sciences, Queen Margaret University, Edinburgh, Scotland, EH21 6UU, UK
Rights & Permissions [Opens in a new window]

Abstract

Type
Abstract
Copyright
Copyright © The Authors 2012

An increase in abdominal adiposity is linked with the development of a low-grade systemic inflammatory response, characterised by altered adipokine secretion and increased plasma oxidative stress. This in turn, is thought to have a role in the increased risk of T2DM and CVD associated with obesity and interventions are therefore sought which are able to modulate plasma adipokine concentrations, re-establishing a healthy balance.

It was hypothesised that consumption of a pomegranate extract (PE) for 28 d by a healthy, overweight/obese (BMI=29.2 sd 3.1; Age=37.1 sd 10.2) population would alter circulating adipokines and plasma total antioxidant status. A randomised, placebo-controlled, single-blind crossover design was used, with a 14 d washout period. Measurements were taken at baseline and 28 d for each intervention, within 24 hours of the last capsule being taken. No significant changes in energy or macronutrient intake (Fig. A & B), assessed using a 3 d diet diary, BMI, waist circumference or waist:hip ratio were noted for either treatment group. Consumption of PE for 28 d did not significantly increase plasma antioxidant levels as measured by the ferric reducing antioxidant capacity (FRAP)()Reference Benzie and Strain 1 ) or oxygen radical absorbance capacity (ORAC)( Reference Girard-Lalancette, Pichette and Legault 2 ), nor did it affect oxidative stress as measured by plasma thiobarbituric reactive substances assay (TBARS)( Reference Buege and Aust 3 ). TNFα, IL-6, adiponectin and leptin (RnD Systems, UK) did not significantly change for either treatment.

In conclusion, intake of a PE for 28 d did not result in a cumulative increase in plasma total antioxidant capacity or affect plasma oxidative stress. Nor was there any effect on plasma adipokines. These results suggest that consumption of a PE over a 28 d period has no effect on the secretory pattern of adipose tissue in obesity. However it may possibly have some action beyond this time period or at the subcellular level.

This work was supported by POM Wonderful, LLC, USA.

References

1. Benzie, I & Strain, J (1996) The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’. The FRAP assay. Analytical Biochemistry 239, 70–6.CrossRefGoogle ScholarPubMed
2. Girard-Lalancette, K, Pichette, A & Legault, J. (2009) Sensitive cell-based assay using DCFH oxidation for the determination of pro- and antioxidant properties of compounds and mixtures: Analysis of fruit and vegetable juices. Food Chemistry 115(2): 720–6.CrossRefGoogle Scholar
3. Buege, J & Aust, S. (1978) Microsomal lipid peroxidation. Methods Enzymol 52(302): 310.Google ScholarPubMed