Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-26T03:13:24.559Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of n-3 fatty acids on cartilage metabolism

Published online by Cambridge University Press:  28 July 2008

Clare L. Curtis ,
Sarah G. Rees ,
Joanna Cramp ,
Carl R. Flannery ,
Clare E. Hughes ,
Chris B. Little ,
Rhys Williams ,
Chris Wilson ,
Colin M. Dent  and
John L. Harwood
...Show all authors
Clare L. Curtis
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Sarah G. Rees
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Joanna Cramp
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Carl R. Flannery*
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Clare E. Hughes
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Chris B. Little*
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Rhys Williams
Affiliation:
Department of Traumatic and Orthopaedic Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XW, UK
Chris Wilson
Affiliation:
Department of Traumatic and Orthopaedic Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XW, UK
Colin M. Dent
Affiliation:
Department of Traumatic and Orthopaedic Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XW, UK
John L. Harwood
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Bruce Caterson*
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
*
*Present address: Wyeth Research, Cambridge, MA 02140, USA.
†Present address: Melbourne University Department of Paediatrics and Murdoch Children's Research Institute, Parkville 3052, Victoria, Australia.
†Present address: Melbourne University Department of Paediatrics and Murdoch Children's Research Institute, Parkville 3052, Victoria, Australia.
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Although the clinical benefits of dietary supplementation with n-3 polyunsaturated fatty acids (PUFA) has been recognised for a number of years, the molecular mechanisms by which particular PUFA affect metabolism of cells within the synovial joint tissues are not understood. This study set out to investigate how n-3 PUFA and other classes of fatty acids affect both degradative and inflammatory aspects of metabolism of articular cartilage chondrocytes using an in vitro model of cartilage degradation. Using well-established culture models, cartilage explants from normal bovine and human osteoarthritic cartilage were supplemented with either n-3 or n-6 PUFA, and cultures were subsequently treated with interleukin 1 to initiate catabolic processes that mimic cartilage degradation in arthritis. Results show that supplementation specifically with n-3 PUFA, but not n-6 PUFA, causes a decrease in both degradative and inflammatory aspects of chondrocyte metabolism, whilst having no effect on the normal tissue homeostasis. Collectively, our data provide evidence supporting dietary supplementation of n-3 PUFA, which in turn may have a beneficial effect of slowing and reducing inflammation in the pathogenesis of degenerative joint diseases in man.

Keywords

n-3 PUFACartilage metabolismChondrocytesDegenerative diseases
Type
Joint National Nurses Nutritional Group and Parenteral and Enteral Group of the British Dietetic Association Symposium on ‘Working in Partnership’
Information
Proceedings of the Nutrition Society , Volume 61 , Issue 3 , August 2002 , pp. 381 - 389
Copyright
Copyright © The Nutrition Society 2002

References

Abbaszade, I, Liu, R-Q, Yang, F, Rosenfeld, SA, Ross, OH, Link, JR et al. (1999) Cloning and characterization of ADAMTS11, an aggrecanase from the ADAMTS family. Journal of Biological Chemistry 274, 23443–23450.CrossRefGoogle ScholarPubMed
Arner, EC, Hughes, CE, Decicco, CP, Caterson, B & Tortorella, MD (1998) Cytokine-induced cartilage proteoglycan degradation is mediated by aggrecanase. Osteoarthritis Cartilage 6, 214–228.Google Scholar
Brash, AR (1999) Lipoxygenases: occurrence, functions, catalysis and acquisition of substrate. Journal of Biological Chemistry 274, 23679–23682.Google Scholar
Calder, PC (2001) n-3 polyunsaturated fatty acids, inflammation and immunity: pouring oil on troubled waters or another fishy tale? Nutrition Research 21, 309–341.CrossRefGoogle Scholar
Calder, PC & Zurier, RB (2001) Polyunsaturated fatty acids and rheumatoid arthritis. Current Opinion in Clinical Nutrition and Metabolic Care 4, 115–121.Google Scholar
Caterson, B, Flannery, CR, Hughes, CE & Little, CB (2000) Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biology 19, 333–344.Google Scholar
Caterson, B, Hughes, CE, Roughley, PJ & Mort, JS (1995) Anabolic and catabolic markers of proteoglycan metabolism in osteoarthritis. Acta Orthopaedica Scandinavica 66, Suppl. 266, 121–124.CrossRefGoogle Scholar
Cleland, LG & James, MJ (2000) Fish oil and rheumatoid arthritis: anti-inflammatory and collateral health benefits. Journal of Rheumatology 27, 2305–2307.Google Scholar
Curtis, CL, Hughes, CE, Flannery, CR, Little, CB, Harwood, JL & Caterson, B (2000) n-3 fatty acids specifically modulate catabolic factors involved in articular cartilage degradation. Journal of Biological Chemistry 275, 721–724.Google Scholar
Curtis, CL, Rees, SG, Little, CB, Flannery, CR, Hughes, CE, Wilson, C, Dent, CM, Otterness, IG, Harwood, JL & Caterson, B (2002) Pathological indicators of degradation and inflammation in human osteoarthritic cartilage are abrogated by exposure to n-3 fatty acids. Arthritis and Rheumatism (In the Press).Google Scholar
Farndale, RW, Buttle, DJ & Barret, AJ (1986) Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochimica et Biophysica Acta 883, 173–177.CrossRefGoogle ScholarPubMed
Hughes, CE, Caterson, B, Fosang, AJ, Roughley, PJ & Mort, JS (1995) Monoclonal antibodies that specifically recognize neoepitope sequences generated by ‘aggrecanase’ and matrix metalloproteinase cleavage of aggrecan: application to catabolism in situ and in vitro. Biochemical Journal 305, 799–804.CrossRefGoogle ScholarPubMed
Ilic, MZ, Handley, CJ, Robinson, HC & Mok, MT (1992) Mechanism of catabolism of aggrecan by articular cartilage. Archives of Biochemistry and Biophysics 294, 115–122.Google Scholar
Isomaki, P & Punnonen, J (1997) Pro- and anti-inflammatory cytokines in rheumatoid arthritis. Annals of Medicine 29, 499–507.Google Scholar
Lee, TH, Hoover, RL, Williams, JD, Sperling, RL, Ravalese, J, Spur, BW, Robinson, DR, Corey, EJ, Lewis, RA & Austen, KE (1985) Effect of dietary enrichment with eicosapentaenoic and docosahexaenoic acids on in vitro neutrophil and monocyte leukotriene generation and neutrophil function. New England Journal of Medicine 312, 1217–1224.Google Scholar
Little, CB, Flannery, CR, Hughes, CE, Mort, JS, Roughley, PJ, Dent, C & Caterson, B (1999) Aggrecanase versus matrix metalloproteinases in the catabolism of the IGD of aggrecan in vitro. Biochemical Journal 344, 61–68.CrossRefGoogle ScholarPubMed
Little, CB, Hughes, CE, Curtis, CL, Janusz, MJ, Bohne, R, Wang-Weigand, S, Taiwo, YO, Mitchell, PG, Otterness, IG, Flannery, CR & Caterson, B (2002) Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biology 21, 271–288CrossRefGoogle ScholarPubMed
Rees, SG, Flannery, CR, Little, CB, Hughes, CE, Caterson, B & Dent, CM (2000) Catabolism of aggrecan, decorin and biglycan in tendon. Biochemical Journal 350, 181–188.Google Scholar
Reno, C, Marchuk, L, Sciore, P, Frank, CB & Hart, DA (1997) Rapid isolation of total RNA from small samples of hypocellular, dense connective tissues. Biotechniques 22, 1082–1086.CrossRefGoogle ScholarPubMed
Sandy, JD, Neame, PJ, Boynton, RE & Flannery, CR (1991) Catabolism of aggrecan in cartilage explants. Identification of a major cleavage site within the interglobular domain. Journal of Biological Chemistry 266, 8683–8685.CrossRefGoogle Scholar
Sicarde, B & Lagarde, M (1985) Incorporation of some eicosanoic acids into endothelial cells – effect on platelet inhibitory activity and prostacyclin production. Thrombosis and Haemostasis 53, 264–267.Google Scholar
Takita, T, Nakamura, K, Kimira, M, Yamada, N, Kobayashi, Y & Innami, S (1996) Serum fatty acid compositions and lipid concentrations and their correlations. Journal of Clinical Biochemistry and Nutrition 20, 149–159.CrossRefGoogle Scholar
Tortorella, MD, Burn, TC, Pratta, MA, Abbaszade, I, Hollis, JM, Liu, R, et al. (1999) Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins. Science 284, 1664–1666.Google Scholar
Vane, JR, Bakhle, YS & Botting, RM (1998) Cyclooxygenases 1 and 2. Annual Review of Pharmacology and Toxicology 38, 97–120.Google Scholar
Volker, D & Garg, M (1996) Dietary n-3 fatty acid supplementation in rheumatoid arthritis – mechanisms, clinical outcomes, controversies and future directions. Journal of Clinical Biochemistry and Nutrition 20, 83–97.CrossRefGoogle Scholar