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Effect of oil (sunflower oil) consumption with added hydroxytyrosol (natural antioxidant) on antioxidant variables in leucocytes from healthy adults

Published online by Cambridge University Press:  12 May 2008

I. Baeza ,
N. M. de Castro ,
L. E. Díaz ,
A. Marcos  and
M. de la Fuente
I. Baeza
Affiliation:
Departamento de Fisiología Animal, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Spain
N. M. de Castro
Affiliation:
Departamento de Fisiología Animal, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Spain
L. E. Díaz
Affiliation:
Grupo Inmunonutrición, Departamento de Metabolismo y Nutrición, Consejo Superior de Investigaciones Científicas, Madrid, Spain
A. Marcos
Affiliation:
Grupo Inmunonutrición, Departamento de Metabolismo y Nutrición, Consejo Superior de Investigaciones Científicas, Madrid, Spain
M. de la Fuente
Affiliation:
Departamento de Fisiología Animal, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Spain
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Abstract

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Type
1st International Immunonutrition Workshop, Valencia, 3–5 October 2007, Valencia, Spain
Information
Proceedings of the Nutrition Society , Volume 67 , Issue OCE1: 1st International Immunonutrition Workshop, Valencia, 3–5 October 2007, Valencia, Spain , May 2008 , E26
Copyright
Copyright © The Authors 2008

Hydroxytyrosol (3,4-dihydroxyphenylethanol; HT), also known as dihydroxyphenylethanol, is a natural phenolic antioxidant(Reference Papadopoulos and Boskou1, Reference Baldioli, Servili, Perretti and Montedoro2) found in olives and olive oil that is responsible for their antioxidant properties(Reference Carliccio, Siculella, Ancora, Massaro, Scoditti, Storelo, Visioli, Distante and De Caterina3). Although HT is known to exert an antioxidant effect, the mechanism of its action and the identity of the reactive oxygen molecule(s) targeted are not known(Reference ODowd, Driss, Dang, Elbim, Gougerot-Pocidalo, Pasquier and El-Benna4). The aim of the present study was to evaluate the effect of oil (sunflower oil) consumption with ‘Oleoactive from Koipesol’ (Sos Cuetara SA, Madrid, Spain), which contains added HT and is consumed at the level of 45–50 mg/d, on two antioxidant variables (total glutathione levels and glutathione peroxidase (GPx) activity) in leucocytes (neutrophils and lymphocytes) from healthy adults. The design was a randomized cross-over study with twelve healthy subjects of both gender (20–45 years of age). The subjects were divided into two groups. Group A (n 6): 3 weeks of oil (sunflower oil) with added HT; 2 weeks of wash-out; 3 weeks of oil (sunflower oil) without added HT. Group B (n 6): 3 weeks of oil (sunflower oil) without added HT; 2 weeks of wash-out; 3 weeks of oil (sunflower oil) with added HT. The antioxidant variables (total glutathione in lymphocytes and neutrophils and GPx activity in lymphocytes) were analysed before starting oil (sunflower oil) intake and at the three other time-points during the study. In both groups tested the total glutathione levels in lymphocytes did not show significant changes. Nevertheless, in group A glutathione levels in neutrophils and GPx activity in lymphocytes increased in the subjects after the intake of oil (sunflower oil) with added HT (Figure), however no changes were found in group B. In conclusion, HT could protect against oxidative damage since it increases the levels and the activity of two very important antioxidants in the immune cells.

Figure. GPx activity in lymphocytes and total glutathione levels in neutrophils from subjects in group A before (basal) and after 3 weeks of oil (sunflower oil) intake with added HT. Values are means and standard deviations represented by vertical bars. Mean values were significantly different from basal values: ***P<0.001.

This work has been supported by Sos Cuetara of Spain.

References

1. Papadopoulos, G & Boskou, D (1991) J Am Oil Chem Soc 78, 669–671.CrossRefGoogle Scholar
2. Baldioli, M, Servili, M, Perretti, G & Montedoro, GF (1996) J Am Oil Chem Soc 73, 1589–1593.CrossRefGoogle Scholar
3. Carliccio, MA, Siculella, L, Ancora, MA, Massaro, M, Scoditti, E, Storelo, C, Visioli, F, Distante, A & De Caterina, R (2003) Arteroscler Thromb Vasc Biol 23, 622–629.CrossRefGoogle Scholar
4. ODowd, Y, Driss, F, Dang, PM, Elbim, C, Gougerot-Pocidalo, MA, Pasquier, C & El-Benna, J (2004) Biochem Pharmacol 68, 2003–2008.CrossRefGoogle Scholar
Figure 0

Figure. GPx activity in lymphocytes and total glutathione levels in neutrophils from subjects in group A before (basal) and after 3 weeks of oil (sunflower oil) intake with added HT. Values are means and standard deviations represented by vertical bars. Mean values were significantly different from basal values: ***P<0.001.