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Dietary modification of B lymphocytes

Published online by Cambridge University Press:  04 June 2010

B. E. Martínez-Carrillo
Affiliation:
Center for Research and Graduate Studies of Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México Instituto Politécnico Nacional,Laboratorio de Inmunología
R. A. Jarillo-Luna
Affiliation:
Instituto Politécnico Nacional,Laboratorio de Inmunología
V. Rivera-Aguilar
Affiliation:
Instituto Politécnico Nacional,Laboratorio de Inmunología
R. Campos-Rodríguez
Affiliation:
Instituto Politécnico Nacional,Laboratorio de Inmunología
R. V. Pardo-Morales
Affiliation:
Center for Research and Graduate Studies of Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México
C. Rosales-Gómez
Affiliation:
Center for Research and Graduate Studies of Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México
R. Valdés-Ramos
Affiliation:
Center for Research and Graduate Studies of Health Sciences, Faculty of Medicine, Universidad Autónoma del Estado de México
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Abstract

Type
Abstract
Copyright
Copyright © The Authors 2009

The immune system has evolved as a defence mechanism to maintain good health. In recent years, the effect of diet on various aspects of immune function has become increasingly apparent(Reference McGhee, Kunisawa and Kiyono1). These effects have also been observed on some areas of the immune system, however, little is known about changes caused by diet on the gut mucosal immunity, which is the first spot of entry for dietary components and particularly with respect to humoral immunity(Reference Mizubuchi2). The objective of this study was to evaluate the effect of a high fat (HFD) or high carbohydrate (HCD) diet on B and IgA+ lymphocytes in the murine mucosal immune system. Twenty-one day old Balb/c mice were fed a HFD (23.60% lard, 40.26% carbohydrate and protein 23.30%), a HCD (4.3% lard, 66.35% carbohydrate and 18.95% protein) or a Control diet (7.01% lard, 62.94% carbohydrate and protein 20%) during 9 weeks (n=8), after which small intestine and Peyer's patches were obtained, for purification and phenotyping of B-lymphocytes and IgA+ cells by flow cytometry. Mean CD19+ cells in lamina propria for controls was 15.71±2.87%, decreasing in both HFD and HCD (9.09±2.52%, 6.94±1.50% respectively); statistically significant differences were observed by ANOVA (F=29.61, P<0.0001). Peyer's patches showed highest values of CD19+ cells in the controls (69.23±2.41%), followed by HCD and HFD (43.30±6.54% and 50.69±6.06%, respectively), differences were statistically significant (F=71.96 and P<0.0001) (Fig. 1). IgA+ cells in lamina propria were highest in the HCD group (45.57±5.59%), followed by HFD (31.42±5.36%) and Controls (22.66±0.92%) (F=48.97 and P<0.0001). Peyer's patches IgA+ cells were highest in the HFD group (59.05±7.66%), followed by HCD (38.72±5.24) and controls (23.58%±2.14) (ANOVA F=50.20, P<0.0001) (Fig. 2). Both diets decreased CD19+ cells in lamina propria and Peyer's patches, whereas IgA+ cells were higher. It is possible that these dietary modifications are either decreasing the presence of B lymphocytes or accelerating their transformation in IgA producing plasma cells, acting as an antigenic stimulus at the mucosal levels. This project was financed by Universidad Autónoma del Estado de México.

Fig. 1. Percentage of B-lymphocytes (CD19+) in lamina propria and Peyer's patches of Balb/c mice fed with three diets. The values represent the means±sd of three determinations, each group with n=8, ANOVA, P<0.0001. *Post hoc Tukey test comparing the three groups (a, b and, c) showed differences between groups with an IC of 95%, P<0.0001; (a) CL (control), (b) HFD (high fat diet) and (c) HCD (high carbohydrate diet).

Fig. 2. Percentage of IgA+ cells in lamina propria and Peyer's patches in Balb/c mice fed with three diets. The values represent the means±sd of three determinations, each group with n=8, ANOVA, P<0.0001. *Post hoc Tukey test comparing the three groups (a, b and c) showed differences between groups, with an IC of 95%, P<0.0001; (a) CL (control), (b) HFD (high fat diet) and (c) HCD (high carbohydrate diet), IgA (immunoglobulin A).

References

1.McGhee, J, Kunisawa, J & Kiyono, H (2007) Trends in Immunology 28, 150153.CrossRefGoogle Scholar
2.Mizubuchi, H et al. (2005) J Nutr 135, 28572861.CrossRefGoogle Scholar
Figure 0

Fig. 1. Percentage of B-lymphocytes (CD19+) in lamina propria and Peyer's patches of Balb/c mice fed with three diets. The values represent the means±sd of three determinations, each group with n=8, ANOVA, P<0.0001. *Post hoc Tukey test comparing the three groups (a, b and, c) showed differences between groups with an IC of 95%, P<0.0001; (a) CL (control), (b) HFD (high fat diet) and (c) HCD (high carbohydrate diet).

Figure 1

Fig. 2. Percentage of IgA+ cells in lamina propria and Peyer's patches in Balb/c mice fed with three diets. The values represent the means±sd of three determinations, each group with n=8, ANOVA, P<0.0001. *Post hoc Tukey test comparing the three groups (a, b and c) showed differences between groups, with an IC of 95%, P<0.0001; (a) CL (control), (b) HFD (high fat diet) and (c) HCD (high carbohydrate diet), IgA (immunoglobulin A).