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Conjugated linoleic acid feeding during gestation and suckling periods increases antibodies concentration in rat milk

Published online by Cambridge University Press:  04 June 2010

C. Ramírez-Santana ,
F. J. Pérez-Cano ,
M. Castell ,
M. C. López-Sabater ,
M. Rodríguez-Palmero ,
C. Castellote  and
A. Franch
C. Ramírez-Santana
Affiliation:
Department of Physiology, University of Barcelona, Barcelona, Spain CIBER in Epidemiology and Public Health (CIBERESP), Spain
F. J. Pérez-Cano
Affiliation:
Department of Physiology, University of Barcelona, Barcelona, Spain
M. Castell
Affiliation:
Department of Physiology, University of Barcelona, Barcelona, Spain
M. C. López-Sabater
Affiliation:
CIBER in Epidemiology and Public Health (CIBERESP), Spain Department of Nutrition and Food Science, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
M. Rodríguez-Palmero
Affiliation:
Research Department, Ordesa Group, Scientific Park of Barcelona, Barcelona, Spain
C. Castellote
Affiliation:
Department of Physiology, University of Barcelona, Barcelona, Spain CIBER in Epidemiology and Public Health (CIBERESP), Spain
A. Franch
Affiliation:
Department of Physiology, University of Barcelona, Barcelona, Spain
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Abstract

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Abstract
Information
Proceedings of the Nutrition Society , Volume 69 , Issue OCE3: 3rd Immunonutrition Workshop, 21–24 October 2009, Girona, Spain , 2010 , E229
Copyright
Copyright © The Authors 2009

Conjugated linoleic acid (CLA) consists of a mixture of isomers of linoleic acid, of which cis9,trans11 (c9,t11) and trans10,cis12 (t10,c12) predominate. These compounds induce beneficial effects on health and it has been suggested that they may modulate immune responses. Breast milk has Ig and many other bioactive molecules, such as growth factors, cytokines, nucleotides, cellular components and lipids, which promote maturation of the developing immune system. The aim of this study was to determine the effect of feeding 1% CLA on rat milk antibodies concentration and CLA isomers content in pup's plasma.

Pregnant Wistar rats were obtained on day 7 of gestation and were immediately fed 1% CLA pellet chow or standard chow. After delivering, CLA-diet went on during suckling period in a group of CLA mothers (group A), whereas other previous CLA-mothers moved on to standard diet (group B). Litters from group B mothers were daily administered 1% (w/w) CLA by oral gavage. Litters from mothers fed standard chow during gestation were daily administered 1% CLA (group C) or only vehicle (group D). The 1% CLA isomer mixture used was approximately 80% c9,t11 and 20% t10,c12 from the total CLA isomers in oil(Reference Ramírez-Santana, Castellote and Castell1). CLA oil was kindly supplied by Loders Croklaan, Lipid Nutrition, Wormerveer, The Netherlands. Milk was collected from dams on days 2, 5, 9, 14 and 21 post-partum. Total milk was used for CLA isomers quantification by gas chromatography, whereas Ig determination was performed in milk whey by ELISA. ANOVA and post hoc comparisons (Scheffe's test) were performed. Significant differences were accepted at P<0.05.

At the end of suckling period, milk from dams fed CLA diet had higher content of CLA and IgA and IgG concentration than rats fed standard diet (Table 1). At that day, plasma of pups from groups A, B and C showed 9, 12 and 6 times higher content of the c9,t11 CLA isomer than that of group D pups (Table 2). Moreover, similar results were observed in plasma from 14-day-old pups, as well as in milk collected on days 2, 5, 9 and 14 post-partum. In conclusion, supplementation during gestation and suckling with an 80:20 c9,t11:t10,c12 CLA mix, enhances the production of the main Ig isotypes in Wistar rats' milk, which are transferred to pups, as well as CLA.

Table 1

*P<0.05 v. standard diet. n=4–7 dams per group.

Table 2

N.D., non-detectable. Both CLA isomers content in pups’ plasma are significantly different among all groups (one-way ANOVA, P<0.001), n=10.

References

1.Ramírez-Santana, C, Castellote, C, Castell, M et al. (2009) J Nutr 139, 76–81.CrossRefGoogle Scholar
Figure 0

Table 1

Figure 1

Table 2