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Arterio–venous differences to study macronutrient metabolism: introduction and overview

Published online by Cambridge University Press:  12 June 2007

I. A. Macdonald
I. A. Macdonald*
Affiliation:
School of Biomedical Sciences, University of Nottingham Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK
*
Corresponding Author: Professor I. A. Macdonald, fax +44 (0)115 9709259, email [email protected]
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Abstract

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The arterio-venous difference technique is now well established in the study of organ and tissue metabolism. This technique requires samples to be obtained of the arterial blood supplying and the venous drainage from a tissue, together with a measurement of the blood flow through the tissue. The technique is most appropriate when the arterial concentration and tissue metabolism of a substance are constant, and when the blood flow is stable. If these criteria are not satisfied, care is needed in the interpretation of the results obtained. It should be recognized that the arterio-venous difference technique only measures the net exchange of a substance with the tissue, and that tracers are needed if unidirectional flux needs to be estimated. The other factors which must be borne in mind when intending to use this technique are the transit times of blood and the substance of interest through a tissue, the volume of distribution of the substance in the tissue, and the possibility that the venous samples obtained are derived from a mixture of different tissues.

Keywords

Arterio-venous differenceTissue metabolismFick PrincipleArterialization
Type
Meeting Report
Information
Proceedings of the Nutrition Society , Volume 58 , Issue 4 , November 1999 , pp. 871 - 875
Copyright
The Nutrition Society

References

Andres, R, Cader, G, Zierler, KL (1956) The quantitatively minor role of carbohydrate in oxidative metabolism by skeletal muscle in intact man in the basal state. Measurements of oxygen and glucose uptake and carbon dioxide and lactate production in the forearm. Journal of Clinical Investigation 35, 671–682.Google Scholar
Astrup, A, Bulow, J, Christensen, NJ, Madsen, J, Quaade, F (1986) Facultative thermogenesis induced by carbohydrate: a skeletal muscle component mediated by epinephrine. American Journal of Physiology 250, E226–E229.Google Scholar
Astrup, A, Simonsen, L, Bulow, J, Madsen, J, Christensen, NJ (1989) Epinephrine mediates facultative carbohydrate–induced thermogenesis in human skeletal muscle. American Journal of Physiology 257, E340–E345.Google Scholar
Frayn, KN, Coppack, SW, Humphreys, SM, Whyte, PL (1989) Metabolic characteristics of human adipose tissue in vivo. Clinical Science 76, 509–516.CrossRefGoogle ScholarPubMed
Frayn, KN, Macdonald, IA (1997) Assessment of substrate and energy metabolism in vivo. In Clinical Research in Diabetes and Obesity. Part I: Methods, Assessment, and Metabolic Regulation, pp. 101–124 [Draznin, B and Rizza, R, editors]. Totowa, NJ: Humana Press Inc.Google Scholar
Gallen, IW, Fone, KCF, Maggs, DG, Macdonald, IA (1991) The effect of a graded infusion of adrenaline on metabolic rate, forearm electromyographic activity and oxygen consumption. Proceedings of the Nutrition Society 50, 29A.Google Scholar
Gallen, IW, Macdonald, IA (1990) Effect of two methods of hand heating on body temperature, forearm blood flow, and deep venous oxygen saturation. American Journal of Physiology 259, E639–E643.Google Scholar
Hamilton, WF (1962) Measurement of cardiac output. In Handbook of Physiology. Section 2, Circulation, Vol. 1, pp. 551–584 [Hamilton, WF, editor]. Washington, DC: American Physiological Society.Google Scholar
Holling, HE (1939) Observations of the oxygen content of venous blood from the arm vein and on the oxygen consumption of resting human muscle. Clinical Science 4, 103–111.Google Scholar
Landis, EM, Pappenheimer, JR (1963) Exchange of substances through capillary walls. In Handbook of Physiology. Section 2, Circulation, Vol. 2, pp. 961–1034 [Hamilton, WF, editor]. Washington, DC: American Physiological Society.Google Scholar
Liu, D, Andreasson, K, Lins, P-E, Adamson, UC, Macdonald, IA (1993) Adrenaline and noradrenaline responses during insulin-induced hypoglycaemia in man: should the hormone levels be measured in arterialized venous blood? Acta Endocrinologica 128, 95–98.Google Scholar
Liu, D, Moberg, E, Kolling, M, Lins, P-E, Adamson, U, Macdonald;, IA (1992) Arterial, arterialised venous, venous and capillary blood glucose measurements in normal man during hyperinsulinaemic euglycaemia and hypoglycaemia. Diabetologia 35, 287–290.Google Scholar
McGuire, EAH, Helderman, JH, Tobin, JD, Andres, R, Berman, M (1976) Effects of arterial versus venous sampling on analysis of glucose kinetics in man. Journal of Applied Physiology 41, 565–573.CrossRefGoogle ScholarPubMed
Mansell, PI, Macdonald, IA (1990) Effect of starvation on insulin induced glucose disposal and thermogenesis in man. Metabolism 39, 502–510.Google Scholar
Rådegran, G (1999) Limb and skeletal muscle blood flow measurements at rest and during exercise in human subjects. Proceedings of the Nutrition Society 58, 000–000.Google Scholar
Zierler, KL (1961) Theory of the use of arteriovenous concentration differences for measuring metabolism in steady and non-steady states. Journal of Clinical Investigation 40, 2111–2125.Google Scholar