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Proanthocyanidin (PA) content of Calliandra leaves and the effect of polyethylene glycol and pH on complexes formed between Calliandra tannins and Bovine serum albumin (BSA)

Published online by Cambridge University Press:  05 November 2021

M.B. Salawu ,
T. Acamovic ,
C.S. Stewart  and
F.D.DeB. Hovell
M.B. Salawu
Affiliation:
SAC, 581 King Street, Aberdeen AB24 5UD, Scotland, UK Aberdeen University, 581 King Street, Aberdeen AB24 5UD, Scotland, UK Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
T. Acamovic
Affiliation:
SAC, 581 King Street, Aberdeen AB24 5UD, Scotland, UK
C.S. Stewart
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
F.D.DeB. Hovell
Affiliation:
Aberdeen University, 581 King Street, Aberdeen AB24 5UD, Scotland, UK
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Extract

Calliandra is a proteinaceous leguminous browse for ruminants. Its PA content may however reduce its nutritive value. The negative effects of PA can be reduced by treatment with polyethylene glycol (PEG). Tannin-protein complexes are stable at pH values up to the isoelectric point of the individual protein, and may dissociate at lower or higher pH values. The aim of the study was to determine the PA concentration of Calliandra, and to evaluate the effect of PEG and pH on the formation of complexes between BSA and compounds from Calliandra extracted with aqueous methanol.

Leaves (containing the leaflets and petioles) were harvested from Kenya Agricultural Research Institute regional research center, Embu, in the central highlands of Kenya. Leaves were oven dried (50 °C for 48 h) and were milled (1 mm aperture). The top 30 and lower 30 cm leaves were harvested from 2.5 year old plants.

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Programme
Information
Proceedings of the British Society of Animal Science , Volume 1997 , 1997 , pp. 70
Copyright
Copyright © British Society of Animal Science 1997

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References

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